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Title: Laboratory Notebook 112
Creator: Zhang, Zhe, Varelis, Peter
Date: 2010-03-09T22:34:24Z
Description: This laboratory notebook relates to the "Heat Stability of Ricin in Phosphate Buffer System" project with Peter Varelis as the Principle...
Show moreThis laboratory notebook relates to the "Heat Stability of Ricin in Phosphate Buffer System" project with Peter Varelis as the Principle Investigator.
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Title: Personal VTOL Aircraft (semester?), IPRO 317: VTOL aircraft for the Masses IPRO 317 Project Plan F06
Creator: Cerra, Anthony, Howard, Jason, Misurac, Matt, Oh, Daniel, Pajak, William, Patek, Alan, Poli, David, Qadir, Mohammed, Rozier, Paul, Whittlesey, Robert
Date: 2006-12, 2006-12
Description: The first and primary objective of IPRO 317 is to continue advancing the existing prototype towards full flight worthiness. This will be...
Show moreThe first and primary objective of IPRO 317 is to continue advancing the existing prototype towards full flight worthiness. This will be achieved through several overhauls of existing parts, as well as through consultation with outside experts in the radio control field. The team will also implement many new safety features as the prototype progresses toward the ultimate goal of free flight. The second objective of this semester’s IPRO 317 is to pick up where the previous semester left off on the construction of a second prototype design. Many parts have been ordered and are awaiting assembly; however several others must be developed and fabricated. The end result of this objective will produce a second viable alternative vehicle for use in marketing the idea of a VTOL aircraft to the public. The final objective of IPRO 317 is to review and analyze the results of previous semesters regarding the objectives, design goals, and feasibility of a full-scale prototype, including any existing models. Once the design goals of the aircraft are made firm a new model will be created from parts available on the current market.
Deliverables for IPRO 317: Personal VTOL Aircraft for the Fall 2006 semester
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Title: PLANNING FOR HUMAN IMPLANTATION OF A CORTICAL VISUAL PROSTHESIS (Semester Unknown) IPRO 334: PlanningForHumanImplantationOfACorticalVisualProthesisIPRO334BrochureF09
Creator: Bern, David, Chen, Shanyl, Deroo, Mary, Gorski, David, Kelley, Tom, Leasenby, Alex, Li, Harry, Subhani, Maham, Taneja, Aanchal
Date: 2009, 2009-12
Description: We are providing a consultation to the sponsor, the IIT Visual Prosthesis Team, on how to proceed with preparation for human implantation of...
Show moreWe are providing a consultation to the sponsor, the IIT Visual Prosthesis Team, on how to proceed with preparation for human implantation of their intracortical visual prosthesis, which will be accomplished by researching different aspects and requirements for said implantation with an emphasis on subject perspective and selection. The device itself consists of electrode modules implanted in the visual cortex of individuals with blindness. By inducing a current in the modules, the volunteer would potentially perceive phosphenes, or light perceptions similar to camera flash afterimages, which could possibly form functional vision. The sponsor’s study would be focused on developing an assistive device for individuals with complete blindness.
Deliverables
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Title: PARADIGM SHIFT IN REMOTE EDUCATION FOR THE LABORATORY: A SOCIAL APPROACH TO DISTANCE LEARNING
Creator: Hanley, Richard
Date: 2011-12, 2011-12
Description: With modern telecommunication tools the ability for two individuals to work together remotely has greatly improved. Moreover, there have been...
Show moreWith modern telecommunication tools the ability for two individuals to work together remotely has greatly improved. Moreover, there have been great strides made to improve handling medium to large groups of people in real time in remote settings, viz. the teleconference and webinar. However, the status of remote tools for laboratory environments where there is significant hardware and the individuals are more likely to talk amongst themselves in irregular patterns is not nearly as well studied. This thesis examines the way most laboratories in electrical engineering are setup, and creates a model for how to create a remote environment for distance learning. It then discusses the current state of the art, and how such an implementation of the model can be made with such tools. Finally a usability study of the implementation is examined. The primary result of the usability study is that when moving to a remote environment the social paradigm that students and instructors use is likely to change, and simply working on creating a virtual mirror to the laboratory may not work in the future.
M.S. in Electrical Engineering, December 2011
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Title: eMotion (Semester Unknown) IPRO 354
Creator: Roy, Avelo, Suda, Edward, Segre, Alessandro, Young, Jesse, Badrinathan, Vinayak, Domagala, Krystyna, Bassett, Leo, Sustersic, Sara
Date: 2008, 2008-05
Description: eMotion is dedicated to supporting the mental health and well-being of those who are physically apart but emotionally together through the...
Show moreeMotion is dedicated to supporting the mental health and well-being of those who are physically apart but emotionally together through the utilization of wireless technology applied to the Best Friends Forever <3 Necklace (BFF <3).
Deliverables
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Title: TravelFlash (semester?), IPRO 350
Creator: George, Sherine, Raju, Betsy, Campion, Nicholas, Dabney, Matt, Ostapczuk, Alexander
Date: 2004-12, 2004-12
Description: The goal of the IPRO 350F04- Travel Flash team is to perform a feasibility analysis of the electronic travel guide idea. The team plans to...
Show moreThe goal of the IPRO 350F04- Travel Flash team is to perform a feasibility analysis of the electronic travel guide idea. The team plans to attain its goals by performing a competitive analysis of the existing market for travel guides, analyzing the technology for this particular idea and to do a focus group to receive feedback and input for desirable features. The vision is to do a thorough analysis of the idea to determine if it warrants further investment of this team.
Sponsorship: Sky Blue Mobile; Ed Kaplan Entrepreneurial Studies Program
Deliverables for IPRO 350: TravelFlash for the Fall 2004 semester
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Title: Information Design for Plant Management to Predict Equipment Failure (Semester Unknown) IPRO 303: Information Design for Plant Management to Predict Equipment Failure IPRO 303 Poster2 Sp08
Creator: Dodds, Jacob, Erogbogbo, Samad, Fleming, Rachel, Fujimoto, Haruko, Hazariwala, Nirav, Kim, Jihyung, Lee, Sangwook, Mcanally, Arthur, Simons, Ray
Date: 2008, 2008-05
Description: IPRO 303 is working on the user interface for SmartSignal Corporation’s software which predicts equipment failures in coal fired power plants....
Show moreIPRO 303 is working on the user interface for SmartSignal Corporation’s software which predicts equipment failures in coal fired power plants. We are now in the third stage of the project. We are to deliver the user interface of the software based on the studies done by two previous semesters. SmartSignal suggested that we also consider specific elements of the software such as the clear presentation of developing fault information, and the efficient communication system between users. An appropriate UI will utilize SmartSignal’s predictive analysis software to provide an efficient and clear means for power plant personnel to: · Predict or identify equipment faults · Understand the predicted faults · Prioritize the disposition of the predicted faults or highlight urgent or important faults · Reduce the need for plant personnel to have many years of experience or “institutional knowledge” The ultimate goal of this project is to introduce an innovative approach to the user interface which SmartSignal can use for predicting equipment failures in coal fire power plants. To realize this, we set these objectives. · Research and collect information relative to the User Interfaces [UI] from the first hand users of the software while examining the study done by the previous IPRO303. · Create the Requirements Document for the UI in light of the concerns expressed by SmartSignal and the results of research. · Generate several possible UIs based upon the Requirements Document stated above. · Select one of the Several UI for development of details and revise it based upon the input from SmartSignal. · Add details and finalize the design of the selected UI.
Sponsorship: SmartSignal
Deliverables
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Title: The Artificial Pancreas (semester?), IPRO 308: Developing an Artificial Pancreas IPRO 308 Project Plan F06
Creator: Davis, Elena, Miller, Jennifer, Nazim, Mehjabeen, Patel, Sapna, Patel, Shital C., Pathak, Yagna, Ravuri, Venkata, Reddy, Kirthi, Shivakumar, Vidya, Tanner, Ryan, Vinckevicious, Aurimes
Date: 2006-12, 2006-12
Description: The objective of this IPRO is to develop a system that will function as an artificial pancreas and will incorporate an insulin delivery system...
Show moreThe objective of this IPRO is to develop a system that will function as an artificial pancreas and will incorporate an insulin delivery system with a non invasive glucose monitor.
Deliverables for IPRO 308: The Artificial Pancreas for the Fall 2006 semester
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Title: Development of High Bandwidth Powered Resonance Tube Actuators with Feedback Control
Creator: Raman, G., Khanafseh, S., Cain, A. B., Kerschen, E.
Date: 2004-01-22
Publisher: Academic Press Ltd Elsevier Science Ltd
Description: A high bandwidth powered resonance tube (PRT) actuator potentially useful for noise and flow control applications was developed. High...
Show moreA high bandwidth powered resonance tube (PRT) actuator potentially useful for noise and flow control applications was developed. High bandwidth allows use of the same actuator at various locations on an aircraft and over a range of flight speeds. The actuator selected for bandwidth enhancement was the PRT actuator, which is an adaptation of the Hartmann whistle. The device is capable of producing high-frequency and high-amplitude pressure and velocity perturbations for active flow control applications. Our detailed experiments aimed at understanding the PRT phenomenon are complemented by an improved analytical model and direct numerical simulations. We provide a detailed characterization of the unsteady pressures in the nearfield of the actuator using phase averaged pressure measurements. The measurements revealed that propagating fluctuations at 9 kHz were biased towards the upstream direction (relative to the supply jet). A complementary computational study validated by our experiments was useful in simulating the details in the region between the supply jet and the resonance tube where it was difficult to gather experimental data. High bandwidth was obtained by varying the depth of the resonance tube that determines the frequency produced by the device. Our actuator could produce frequencies ranging from 1600 to 15,000 Hz at amplitudes as high as 160 dB near the source. The frequency variation with depth is predicted well by the quarter wavelength formula for deep tubes but the formula becomes increasingly inaccurate as the tube depth is decreased. An improved analytical model was developed, in which the compliance and mass of the fluid in the integration slot is incorporated into the prediction of resonance frequencies of the system. Finally a feedback controller that varied both the resonance tube depth and spacing to converge on a desired frequency was developed and demonstrated. We are optimistic that numerous potential applications exist for such high bandwidth high dynamic range actuators. (C) 2003 Published by Elsevier Science Ltd.
http://dx.doi.org/10.1016/S0022-460X(03)00212-8
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Title: Solar Hydrogen Hybrid System (semester?), IPRO 301
Creator: Baldwin, James, Bacchus, Roger, Chapman, Colleen, Condei, Michael, Cucci, Brent, Jamal, Waqas, Luong, Anh, Melo, Leonardo, Mahmoud, Mohammad, Newberg, Phil
Date: 2004-12, 2004-12
Description: The photovoltaic/hydrogen hybrid energy system IPRO was started eight years ago. A group of students with their faculty advisor began a...
Show moreThe photovoltaic/hydrogen hybrid energy system IPRO was started eight years ago. A group of students with their faculty advisor began a semester to investigate the use of solar energy to power an electric sign. Since that time, a significant amount of progress has been made. The project progressed from the design phase to the laboratory phase. Once functioning, it was transferred to the roof of the Co-Gen plant on the IIT main campus. During this phase of the project, Phase I, a photovoltaic system was set up that included several solar panels on the roof. Also, a large LED sign was installed on the roof. An array of power management devices were set up at the project site inside the Co-Gen plant. Phase II of the project was planned in order to incorporate an electrolyzer and fuel cell as an energy back up system in conjunction with the PV system. Although these components exist at the work site, they are not integrated into the complete system and are merely there to demonstrate using hydrogen to power an electric load.
Deliverables for IPRO 301: Solar Hydrogen Hybrid System for the Fall 2004 semester
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Title: Zero Energy Lab (Semester Unknown) IPRO 337: Zero Energy Lab IPRO 337 Abstract F08
Creator: Aouissa, Abdelmalek, Chiu, Chris, Dodgen, James, Gambrell, Jennifer, Kadzban, Adam, Kedzuch, Andy, Larkin, Evan, Lowe, Scott, Meyer, James, Rojas, Brian, Vincent, Oba, Wolfe, Clark, Yaker, Farouk
Date: 2008, 2008-12
Description: The IPRO 337 team’s objectives for this semester are to make further progress in updating the top floor of Machinery Hall into a lab for...
Show moreThe IPRO 337 team’s objectives for this semester are to make further progress in updating the top floor of Machinery Hall into a lab for future energy and lighting technology research. The research conducted this semester will help us transform the space into a more sustainable working environment. Since the ultimate goal is rather ambitious, our goal for this particular semester is to implement an energy plan which will become the basis off of which the future IPRO’s will continue towards the ultimate goal. We also intend to clear the space so that the developed plan can be tested.
Deliverables
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Title: High School in Pignon, Haiti (semester?), IPRO 326: High School in Pignon Haiti IPRO 326 Midterm Report S07
Creator: Bailey, Nick, Diaz De Leon Orraca, Frederico, Dike, Chukwuderaa, Franklin, Casey, Goldsmith, Katherine, Hogan, Brian, Igbokwe, Chinedu, Jaromin, Sebastian, Kirsch, Joseph, Lowe, Scott, Samuels, Janina, Velichkov, Veselin, Watkins, Brett, Williams, Allisyn
Date: 2007-07, 2007-07
Description: The IPRO 326 team’s objective for this semester is to design a High School so an additional 400 children will have access to affordable...
Show moreThe IPRO 326 team’s objective for this semester is to design a High School so an additional 400 children will have access to affordable education in the remote town of Pignon, Haiti. We want to assist our partner Haiti Outreach in realizing this goal by providing them technical assistance, project management and costing tools as well as advice and guidelines that will improve their construction techniques.
Sponsorship: Haiti Outreach IIT Student Chapter
Deliverables for IPRO 326: High School in Pignon, Haiti for the Summer 2007 semester
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Title: EUTECTIC γ(NI)/γ′(NI3AL)-δ(NI3NB) POLYCRYSTALLINE NICKEL-BASE SUPERALLOYS: CHEMISTRY, PROCESSING, MICROSTRUCTURE AND PROPERTIES
Creator: Xie, Mengtao
Date: 2012-12-03, 2012-12
Description: Directionally solidified γ(Ni)/γ′(Ni3Al)-δ(Ni3Nb) eutectic alloys possess attrac- tive high temperature mechanical properties and were...
Show moreDirectionally solidified γ(Ni)/γ′(Ni3Al)-δ(Ni3Nb) eutectic alloys possess attrac- tive high temperature mechanical properties and were considered as candidate tur- bine blade materials. Currently, the properties of polycrystalline γ/γ′-δ alloys are of interest as they inherit many advantageous attributes from the directionally solidi- fied γ/γ′-δ alloys, including high volume fraction of reinforcing phases, exceptional thermal stability and resistance to segregation-induced defect formation. If these at- tributes are properly harnessed, these γ/γ′-δ eutectic alloys might provide a unique solution to the problems experienced by traditional γ/γ′ polycrystalline Ni-base su- peralloys. This thesis is therefore dedicated towards the development of a funda- mental understanding of this novel class of eutectic alloys from several important perspectives. To enrich our understanding of this alloy system, this thesis will first be focused on quantifying the specific effect of individual alloying element on this γ/γ′-δ eutectic system. A set of quaternary Ni-Cr-Al-Nb alloy compositions with increasing levels of Chromium(Cr) was designed to investigate the detailed influence of this element on the primary phase formation, solidus and liquidus temperatures and γ-δ eutectic morphology. The alloying effect of Tantalum(Ta), which shares many similarities to Niobium(Nb), was studied by designing a matrix of multi-component γ/γ′-δ alloy compositions with nominally the same overall (Ta+Nb) content but varying Ta/Nb ratios. Here, different solidification segregation and solid state partitioning behaviors of Ta and Nb in this γ/γ′-δ eutectic system will be discussed, as well as the influ- ence of Ta/Nb ratio on solidification characteristics and equilibrium/non-equilibrium phase volume fractions. Thermodynamic calculations using the Computherm Pandat database (PanNi7) were compared to experimental results in these investigations. The second part of this thesis will aim to provide a more general understand- xvii ing of the effect of various alloying elements, including Cr, Co, Al, Ti, Mo, W, Ta and Nb, on this γ/γ′-δ system. A large number of experimental γ/γ′-δ alloys covering a broad range of compositions was selected for the analysis in this study. Important alloy attributes, such as primary phase formation, overall δ volume fraction, phase transformation temperatures and ternary eutectic initiation, were quantitatively char- acterized as a function of individual alloying element concentrations or combined con- tent of more elements. Linear regression analysis was performed to reveal the relative effectiveness of these elements on this eutectic system. Meanwhile, an extensive com- parison between the experimental observations and Pandat predictions was provided to critically evaluate the strength and weakness of existing thermodynamic database model in predicting trends in this eutectic alloy system with substantially higher Nb content compared to traditional γ/γ′ superalloys. The last part of this thesis emphasizes the development of cast and wrought manufacturing processes for cast γ/γ′-δ eutectic alloys as a cost effective alternative to the powder metallurgy route. Hot rolling of workpieces encapsulated within a steel can was performed on a simple model cast γ/γ′-δ alloy (897) to stimulate the ingot to billet. The influence of different deformation levels on breaking down the dendritic structure and promoting fine and homogenized microstructure was investi- gated. The mechanical soundness associated with different microstructures generated by different hot rolling processes was compared via compression and creep testing. Microstructural parameters that contribute to better mechanical properties will be discussed.
PH.D in Materials Science and Engineering, December 2012
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Title: Continiuous improvements of the Global Supply Chain for a Plumbing Systems Manufacturer (Semester Unknown) IPRO 306: ContinuousImprovementsOfTheGlobalSupplyChainForAPlumbingSystemsManufacturerIPRO306ProjectPlanSp09
Creator: Avanessian, Aris, Carbayo, Nestor, Carrio, Aris, Verma, Vibhor, Espinosa, Juan Carlos, Gherardini, Scott, Medina Rivera, Mauricio, Panjwani, Varsha, Rodriguez, Fernando, Schreiner, Stephen
Date: 2009, 2009-05
Description: The IPRO 306 team for the Spring '09 semester is sponsored by Sloan Valve Company, a long-standing family owned company, to assist in supply...
Show moreThe IPRO 306 team for the Spring '09 semester is sponsored by Sloan Valve Company, a long-standing family owned company, to assist in supply chain operations. Sloan Valve wishes to both streamline it's processes and increase employee training and understanding of quality control systems. To this end, the IPRO team will assist them on these two projects.
Sponsorship: SLOAN Valve Company
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Title: Improving Global Supply Chain Management (Semester Unknown) IPRO 306: ImprovingGlobalSupplyChainManagementIPRO306FinalReportSp10_redacted
Creator: Thukral, Anand, Wilas, Arthur, Lukic, Djordje, Smith, Suni, Chandnani, Abhishek, Devalam, Arun, Limpinyakul, Jutarop, Malpekar, Nilesh, Sun, Cong, Kotha, Anurag, Skiba, David, Sharangdhar, Kunal, Bergland, Tomas
Date: 2010, 2010-05
Description: The IPRO 306 team will be working with a sanitation and janitorial equipment manufacturer, Sloan Valve. The core competency of this company...
Show moreThe IPRO 306 team will be working with a sanitation and janitorial equipment manufacturer, Sloan Valve. The core competency of this company lies in manufacturing flush valves. Sloan Valve is currently one of the leading producers of flush valves in the Northern American Continent. The team will be assisting Sloan Valve in realizing two major projects.
Sponsorship: SLOAN
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Title: Evaluating the Commercial Potential of IIT's Mercury Pollution Prevention Technology (semester?), IPRO 356: Mercury Pollution Prevention Tech IPRO 356 Abstract Sp05
Creator: Macdougall, Chris, Nguyen, Kheim, Dabney, Matthew, Wessely, Noel, Kim, Byung, Change, Wen-ya
Date: 2005-05, 2005-05
Description: Our objective in this IPRO is to develop a business plan for Dr. Clack’s VSB technology. In developing this business plan, we are looking at...
Show moreOur objective in this IPRO is to develop a business plan for Dr. Clack’s VSB technology. In developing this business plan, we are looking at the marketing strategy, market size, finances, and competition for this product. We are also looking at the government regulations governing this type of technology, at the business risks in developing the VSB and how to market it. The last objective for this IPRO is to develop a set of objectives and a path forward for any future IPROs on this topic.
Sponsorship: Sargent & Lundy LLC.; Ed Kaplan Entrepreneurial Studies Program
Deliverables for IPRO 356: Evaluating the Commercial Potential of IIT's Mercury Pollution Prevention Technology for the Spring 2005 semester
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Title: SURVIVAL AND GROWTH OF LISTERIA MONOCYTOGENES IN CHOPPED GREEN AND RED BELL PEPPERS USING PREDICTIVE MODELING
Creator: Zhang, L1jie
Date: 2015, 2015-07
Description: Listeria monocytogenes (L. monocytogenes) is a Gram-positive pathogenic organism and the causative agent of human and animal listeriosis....
Show moreListeria monocytogenes (L. monocytogenes) is a Gram-positive pathogenic organism and the causative agent of human and animal listeriosis. Listeriosis is a gastrointestinal or invasive systemic illness resulting from consumption of contaminated food products, mainly cheese, deli meats, and fresh produce, by L. monocytogenes. In recent years, several foodborne outbreaks have been reported that were associated with fresh produce, such as cantaloupe, celery and sprouts. Temperature is considered a major factor that affects L. monocytogenes growth during storage. The proliferation of L. monocytogenes varies on different produce items based on storage temperature. In this study, the persistence and population dynamics of three L. monocytogenes strains, LS806 (cheese isolate), LS810 (cantaloupe isolate) and LS808 (celery isolate) were evaluated by incubating inoculated fresh-cut green bell pepper and red bell pepper at various temperatures (5oC, 10oC, and 25oC) for 14 days. To assess the risk of L. monocytogenes in these fresh-cut vegetable items, a primary predictive model was fitted for L. monocytogenes growth data using DMFit. Green bell pepper had significantly (P<0.05) higher pH and aw, and higher amounts of yeast and mold and Enterobacteriaceae than did red bell pepper. In green bell pepper, all three strains showed no significant difference (P>0.05) in growth rate when incubated at the same temperature. In red bell peppers, LS808 had the highest (P<0.05) growth rate at both 5°C and 25°C out of the three strains. All of the three strains grew significantly faster (P<0.01) at 25°C than either 5°C or 10°C in both green and red bell peppers. All three strains obtained less than 1 log10 growth increase after incubating at 25°C for 6 hours on pre-chilled produce. Some strains (LS806 and LS810) significantly increased (P<0.01) during two 5-hour 25°C incubations, but did not reach 1 log10 growth increase. The results indicate that L. monocytogenes not only persists, but also grows in chopped green and red bell peppers at 5, 10, and 25°C, and strains grew faster at the higher temperature (25°C). Data obtained could be further evaluated for determining whether Time/temperature control for safety (TCS) designation should be applied to chopped green and red bell peppers.
M.S. in Food Safety and Technology, July 2015
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Title: User Created Map Content (Spring 2011) IPRO 305: UserCreatedMapContentIPRO305FinalPresentationSp11
Creator: Ciarkowski, Adam, Hwangbo, James, Jurewicz, Marc, Kaufhold, Kenneth, Kornika, David, Kumar, Arjun, Millonzi, Roger, Paradiso, Nicholas, Simmonds, Claire, Summers, Devon, Yonezawa, Samuel
Date: 2011, 2011-05
Description: NAVTEQ is the corporate sponsor for IPRO 305. NAVTEQ is one of the world’s largest digital mapping companies. With competitors, such as Google...
Show moreNAVTEQ is the corporate sponsor for IPRO 305. NAVTEQ is one of the world’s largest digital mapping companies. With competitors, such as Google, NAVTEQ aims to outdo the competition by providing their clients with robust quality mapping data. NAVTEQ achieves this by employing over 1,000 geographic analysts who’s sole job is to ensure that the data on their maps is unique and, above all, accurate from each of NAVTEQ’s 80,000+ sources[citation 1]. On their quest to provide their clients with robust data, NAVTEQ has identified the need for user- generated content. This is data that a user of a GPS mapping system can enter in for themselves. With this recognition of usergenerated mapping data, NAVTEQ pursues the need to be the world’s most robust digital mapping data provider.
Sponsorship: NAVTEQ
Deliverables
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Title: Influence of the target on multiparticle production in the forward domain in p+Pb collisions at 158 GeV, Proceedings of the XLIII International Symposium on Multiparticle Dynamics
Creator: Rybczyński, Maciej
Date: 2013-09-15, 2013-09-15
Publisher: IIT Press
Description: In this talk we show the influence of the target on multiparticle production in the forward hemisphere in p+Pb collisions at top SPS energy....
Show moreIn this talk we show the influence of the target on multiparticle production in the forward hemisphere in p+Pb collisions at top SPS energy. The multiplicity distributions appear to be almost target independent in the projectile fragmentation domain and the effect of fluctuations of the number of target participants is not seen in the projectile fragmentation region. We compare the obtained results with those for p+p interactions and predictions of models.
Sponsorship: IIT College of Science, High Energy Physics Division of Argonne National Laboratory
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Title: STRUCTURAL CHARACTERIZATION OF THE FLIGHT MUSCLE OF MANDUCA SEXTA
Creator: Cheng, Yu-shu
Date: 2013-04-22, 2013-05
Description: There are several properties of the flight muscle Manduca sexta that are interesting. In its physiological characteristics, like mammalian...
Show moreThere are several properties of the flight muscle Manduca sexta that are interesting. In its physiological characteristics, like mammalian skeletal and cardiac striated muscle, it is a synchronous muscle. However, it is much more similar structurally to the more widely known asynchronous insect flight muscles of Drosophila and Lethocerus. The goal of the thesis is to study the structural characteristics and perform mechanical studies of dorsal and ventral muscle of Manduca flight muscle. In pursuit of this goal, a secondary purpose was to develop better storage conditions for skinned insect muscle which can maintain muscle structure and function. Maximum active force as a function of time in storage was compared for storing at 4℃ without glycerol and storing at -80℃ with 75% glycerol. The maximum force values were almost the same during days 0-4. However, the muscle stored at -80℃ with 75% glycerol gave higher active force in high calcium (pCa 4.5) solution over a longer time in storage than muscle stored at 4℃. Both ventral and dorsal muscles can deliver 40-50% of original active force for up to 21 days in storage. X-ray diffraction experiments were done to compare the response of chemically skinned Manduca flight muscle to temperature and to the induction of rigor. The lattice spacing (d10) decreased and intensity ratio I20/I10 increased when the temperature of the relaxing solution bathing the solution increased from 10℃ to 40℃. Lattice spacing (d10) decreased, but the I20/I10 intensity ratio increased slightly while concentration of dextran increased from 1% to 6%. Six percent dextran was insufficient to restore the in vivo lattice spacing.
M.S. in Molecular Biochemistry and Biophysics, May 2013
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