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Title: BIFUNCTIONAL CHELATING AGENTS FOR POSITRON EMISSION TOMOGRAPHY AND RADIOIMMUNOTHERAPY
Creator: Sin, In Seok
Date: 2013, 2013-12
Description: Positron emission tomography (PET) is an important molecular imaging modality. To develop new bifunctional chelators for use in more sensitive...
Show morePositron emission tomography (PET) is an important molecular imaging modality. To develop new bifunctional chelators for use in more sensitive PET imaging, 64Cu-radiolabeled chelators have been investigated as promising PET agents. We synthesized and evaluated a macrocyclic chelator (NOTA)-based new bifunctional ligands with different coordination groups. New bifunctional chelators were evaluated for their radiolabeling efficiency with 64Cu, and in vitro complex stability of 64Cu-labeled chelators in human serum was also studied. 64Cu-labeled chelators was further evaluated for in vitro complex stability by EDTA challenge as a rigorous test of transchelation. As a result, chelator D, E, 3p-C-NE3TA, and 3p-C-NOTA were indicated as best chelators and have a promise for 64Cu-based PET imaging. Radioimmunotherapy (RIT) is a highly selective modality of cancer treatment and uses a radiolabeled chelator conjugated with monoclonal antibody. Our continued research of developing new RIT agents radiolabeled with 177Lu, different types of a macrocyclic based bifunctional chelators (DOTA, NOTA and NE3TA)- with variation of denticity, macrocyclic cavity, and bimodality were synthesized. Radiolabeling efficiency of chelators and in vitro complex stability of 177Lu-radiolabeled complex in human serum were evaluated. The effects of different pH and TLC solvents were investigated to confirm the best radiolabeling condition of new chelators with 177Lu. Furthermore, complex stability of 177Lu-radiolabeled chelators in a solution with excess EDTA and various metals was evaluated for transchelation. Therefore, the results of radiolabeling, serum stability and EDTA and metal challenge studies indicate that cavity size and ligand x denticity in chelators affected binding affinity to Lu(III) . 3p-C-DETA was identified as the best chelator for further research of RIT agent.
M.S. in Chemistry, December 2013
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Title: DEVELOPMENT OF AN ARTIFICIAL PANCREAS (Semester Unknown) IPRO 308
Creator: Klug, Allen, Kuuspalu, Adam, Smith, Adam, Naveenan, Anju, Sin, In Seok, Sonoiki, Olufemi, Wakeman, William
Date: 2008, 2008-12
Description: Our team was asked to come up with an innovative and non-invasive means of extracting and measuring glucose levels in the body as well as...
Show moreOur team was asked to come up with an innovative and non-invasive means of extracting and measuring glucose levels in the body as well as administering insulin back into the body, also by non-invasive means. Our research lead us to several reports on the effectiveness of ultrasound in expanding pore opening diameters. We decided to approach the problem with a device that would administer ultrasonic vibration to the surface of the skin thus expanding the pore openings. Once expanded a combination of vacuum and reverse iontophoresis would then extract a small amount of interstitial fluid. The fluid would be measured for glucose concentration using impedance spectroscopy. Once a glucose concentration was found, a proper amount of insulin would then be administered back through the skin by means of pressure and iontophoresis. Our experiments were conducted on harvested porcine skin. Sonic vibration at or around 10kHz served as a substitute for ultrasound. Vacuum was administered to the surface of the skin after a set amount of time of sonic vibration. Although results at first seemed promising, further investigation into the behavior of harvested skin yielded that our results were not as they seemed. Impedance spectroscopy was explored by first trying to find the resonant frequency of glucose. Earlier attempts at this were unsuccessful do to frequency limitations of measuring devices. Our team learned of a device that could measure 10 times higher frequencies than what had previously been recorded, but unfortunately was unable to solidify a concrete resonant frequency for glucose.
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Title: DEVELOPMENT OF AN ARTIFICIAL PANCREAS (Semester Unknown) IPRO 308: Creating an Artificial Pancreas IPRO 308 Final Report F08
Creator: Klug, Allen, Kuuspalu, Adam, Smith, Adam, Naveenan, Anju, Sin, In Seok, Sonoiki, Olufemi, Wakeman, William
Date: 2008, 2008-12
Description: Our team was asked to come up with an innovative and non-invasive means of extracting and measuring glucose levels in the body as well as...
Show moreOur team was asked to come up with an innovative and non-invasive means of extracting and measuring glucose levels in the body as well as administering insulin back into the body, also by non-invasive means. Our research lead us to several reports on the effectiveness of ultrasound in expanding pore opening diameters. We decided to approach the problem with a device that would administer ultrasonic vibration to the surface of the skin thus expanding the pore openings. Once expanded a combination of vacuum and reverse iontophoresis would then extract a small amount of interstitial fluid. The fluid would be measured for glucose concentration using impedance spectroscopy. Once a glucose concentration was found, a proper amount of insulin would then be administered back through the skin by means of pressure and iontophoresis. Our experiments were conducted on harvested porcine skin. Sonic vibration at or around 10kHz served as a substitute for ultrasound. Vacuum was administered to the surface of the skin after a set amount of time of sonic vibration. Although results at first seemed promising, further investigation into the behavior of harvested skin yielded that our results were not as they seemed. Impedance spectroscopy was explored by first trying to find the resonant frequency of glucose. Earlier attempts at this were unsuccessful do to frequency limitations of measuring devices. Our team learned of a device that could measure 10 times higher frequencies than what had previously been recorded, but unfortunately was unable to solidify a concrete resonant frequency for glucose.
Deliverables
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Title: DEVELOPMENT OF AN ARTIFICIAL PANCREAS (Semester Unknown) IPRO 308: Creating an Artificial Pancreas IPRO 308 Poster F08
Creator: Klug, Allen, Kuuspalu, Adam, Smith, Adam, Naveenan, Anju, Sin, In Seok, Sonoiki, Olufemi, Wakeman, William
Date: 2008, 2008-12
Description: Our team was asked to come up with an innovative and non-invasive means of extracting and measuring glucose levels in the body as well as...
Show moreOur team was asked to come up with an innovative and non-invasive means of extracting and measuring glucose levels in the body as well as administering insulin back into the body, also by non-invasive means. Our research lead us to several reports on the effectiveness of ultrasound in expanding pore opening diameters. We decided to approach the problem with a device that would administer ultrasonic vibration to the surface of the skin thus expanding the pore openings. Once expanded a combination of vacuum and reverse iontophoresis would then extract a small amount of interstitial fluid. The fluid would be measured for glucose concentration using impedance spectroscopy. Once a glucose concentration was found, a proper amount of insulin would then be administered back through the skin by means of pressure and iontophoresis. Our experiments were conducted on harvested porcine skin. Sonic vibration at or around 10kHz served as a substitute for ultrasound. Vacuum was administered to the surface of the skin after a set amount of time of sonic vibration. Although results at first seemed promising, further investigation into the behavior of harvested skin yielded that our results were not as they seemed. Impedance spectroscopy was explored by first trying to find the resonant frequency of glucose. Earlier attempts at this were unsuccessful do to frequency limitations of measuring devices. Our team learned of a device that could measure 10 times higher frequencies than what had previously been recorded, but unfortunately was unable to solidify a concrete resonant frequency for glucose.
Deliverables
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Title: DEVELOPMENT OF AN ARTIFICIAL PANCREAS (Semester Unknown) IPRO 308: Creating an Artificial Pancreas IPRO 308 Ethics F08
Creator: Klug, Allen, Kuuspalu, Adam, Smith, Adam, Naveenan, Anju, Sin, In Seok, Sonoiki, Olufemi, Wakeman, William
Date: 2008, 2008-12
Description: Our team was asked to come up with an innovative and non-invasive means of extracting and measuring glucose levels in the body as well as...
Show moreOur team was asked to come up with an innovative and non-invasive means of extracting and measuring glucose levels in the body as well as administering insulin back into the body, also by non-invasive means. Our research lead us to several reports on the effectiveness of ultrasound in expanding pore opening diameters. We decided to approach the problem with a device that would administer ultrasonic vibration to the surface of the skin thus expanding the pore openings. Once expanded a combination of vacuum and reverse iontophoresis would then extract a small amount of interstitial fluid. The fluid would be measured for glucose concentration using impedance spectroscopy. Once a glucose concentration was found, a proper amount of insulin would then be administered back through the skin by means of pressure and iontophoresis. Our experiments were conducted on harvested porcine skin. Sonic vibration at or around 10kHz served as a substitute for ultrasound. Vacuum was administered to the surface of the skin after a set amount of time of sonic vibration. Although results at first seemed promising, further investigation into the behavior of harvested skin yielded that our results were not as they seemed. Impedance spectroscopy was explored by first trying to find the resonant frequency of glucose. Earlier attempts at this were unsuccessful do to frequency limitations of measuring devices. Our team learned of a device that could measure 10 times higher frequencies than what had previously been recorded, but unfortunately was unable to solidify a concrete resonant frequency for glucose.
Deliverables
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Title: DEVELOPMENT OF AN ARTIFICIAL PANCREAS (Semester Unknown) IPRO 308: Creating an Artificial Pancreas IPRO 308 MidTerm Presentation F08
Creator: Klug, Allen, Kuuspalu, Adam, Smith, Adam, Naveenan, Anju, Sin, In Seok, Sonoiki, Olufemi, Wakeman, William
Date: 2008, 2008-12
Description: Our team was asked to come up with an innovative and non-invasive means of extracting and measuring glucose levels in the body as well as...
Show moreOur team was asked to come up with an innovative and non-invasive means of extracting and measuring glucose levels in the body as well as administering insulin back into the body, also by non-invasive means. Our research lead us to several reports on the effectiveness of ultrasound in expanding pore opening diameters. We decided to approach the problem with a device that would administer ultrasonic vibration to the surface of the skin thus expanding the pore openings. Once expanded a combination of vacuum and reverse iontophoresis would then extract a small amount of interstitial fluid. The fluid would be measured for glucose concentration using impedance spectroscopy. Once a glucose concentration was found, a proper amount of insulin would then be administered back through the skin by means of pressure and iontophoresis. Our experiments were conducted on harvested porcine skin. Sonic vibration at or around 10kHz served as a substitute for ultrasound. Vacuum was administered to the surface of the skin after a set amount of time of sonic vibration. Although results at first seemed promising, further investigation into the behavior of harvested skin yielded that our results were not as they seemed. Impedance spectroscopy was explored by first trying to find the resonant frequency of glucose. Earlier attempts at this were unsuccessful do to frequency limitations of measuring devices. Our team learned of a device that could measure 10 times higher frequencies than what had previously been recorded, but unfortunately was unable to solidify a concrete resonant frequency for glucose.
Deliverables
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Title: DEVELOPMENT OF AN ARTIFICIAL PANCREAS (Semester Unknown) IPRO 308: Creating an Artificial Pancreas IPRO 308 Brochure F08
Creator: Klug, Allen, Kuuspalu, Adam, Smith, Adam, Naveenan, Anju, Sin, In Seok, Sonoiki, Olufemi, Wakeman, William
Date: 2008, 2008-12
Description: Our team was asked to come up with an innovative and non-invasive means of extracting and measuring glucose levels in the body as well as...
Show moreOur team was asked to come up with an innovative and non-invasive means of extracting and measuring glucose levels in the body as well as administering insulin back into the body, also by non-invasive means. Our research lead us to several reports on the effectiveness of ultrasound in expanding pore opening diameters. We decided to approach the problem with a device that would administer ultrasonic vibration to the surface of the skin thus expanding the pore openings. Once expanded a combination of vacuum and reverse iontophoresis would then extract a small amount of interstitial fluid. The fluid would be measured for glucose concentration using impedance spectroscopy. Once a glucose concentration was found, a proper amount of insulin would then be administered back through the skin by means of pressure and iontophoresis. Our experiments were conducted on harvested porcine skin. Sonic vibration at or around 10kHz served as a substitute for ultrasound. Vacuum was administered to the surface of the skin after a set amount of time of sonic vibration. Although results at first seemed promising, further investigation into the behavior of harvested skin yielded that our results were not as they seemed. Impedance spectroscopy was explored by first trying to find the resonant frequency of glucose. Earlier attempts at this were unsuccessful do to frequency limitations of measuring devices. Our team learned of a device that could measure 10 times higher frequencies than what had previously been recorded, but unfortunately was unable to solidify a concrete resonant frequency for glucose.
Deliverables
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Title: DEVELOPMENT OF AN ARTIFICIAL PANCREAS (Semester Unknown) IPRO 308: Creating an Artificial Pancreas IPRO 308 Final Presentation F08
Creator: Klug, Allen, Kuuspalu, Adam, Smith, Adam, Naveenan, Anju, Sin, In Seok, Sonoiki, Olufemi, Wakeman, William
Date: 2008, 2008-12
Description: Our team was asked to come up with an innovative and non-invasive means of extracting and measuring glucose levels in the body as well as...
Show moreOur team was asked to come up with an innovative and non-invasive means of extracting and measuring glucose levels in the body as well as administering insulin back into the body, also by non-invasive means. Our research lead us to several reports on the effectiveness of ultrasound in expanding pore opening diameters. We decided to approach the problem with a device that would administer ultrasonic vibration to the surface of the skin thus expanding the pore openings. Once expanded a combination of vacuum and reverse iontophoresis would then extract a small amount of interstitial fluid. The fluid would be measured for glucose concentration using impedance spectroscopy. Once a glucose concentration was found, a proper amount of insulin would then be administered back through the skin by means of pressure and iontophoresis. Our experiments were conducted on harvested porcine skin. Sonic vibration at or around 10kHz served as a substitute for ultrasound. Vacuum was administered to the surface of the skin after a set amount of time of sonic vibration. Although results at first seemed promising, further investigation into the behavior of harvested skin yielded that our results were not as they seemed. Impedance spectroscopy was explored by first trying to find the resonant frequency of glucose. Earlier attempts at this were unsuccessful do to frequency limitations of measuring devices. Our team learned of a device that could measure 10 times higher frequencies than what had previously been recorded, but unfortunately was unable to solidify a concrete resonant frequency for glucose.
Deliverables
Show less