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(1 - 15 of 15)
Remarks to the Board of Trustees
Title
Remarks to the Board of Trustees
Creator
Mitchell, M. Ellen
Date
2009
Description
Presentation notes for remarks to the Board of Trustees regarding the Voices of the Holocaust project.
Systems and Systematic Design: Tracing the Evolution of Design Methodology at the Institute of Design, 1965-2010: Video documentation
Title
Systems and Systematic Design: Tracing the Evolution of Design Methodology at the Institute of Design, 1965-2010: Video documentation
Creator
Owen, Charles L.
Date
2010-10-28
Description
The 2010 IIT Archives annual lecture was scheduled on Thursday, October 28 from 3:30–5 p.m., with a reception immediately following. The...
Show moreThe 2010 IIT Archives annual lecture was scheduled on Thursday, October 28 from 3:30–5 p.m., with a reception immediately following. The lecture and reception was held in the McCloska Auditorium and Ballroom, located in The McCormick Tribune Campus Center.
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Systems and Systematic Design: Tracing the Evolution of Design Methodology at the Institute of Design, 1965-2010
Title
Systems and Systematic Design: Tracing the Evolution of Design Methodology at the Institute of Design, 1965-2010
Creator
Owen, Charles L.
Date
2010-10-28
They Aren't Like Me, They are Bad, and They are to Blame: A Theoretically-Informed Study of Stigma of Hoarding Disorder and Obsessive-Compulsive Disorder
Title
They Aren't Like Me, They are Bad, and They are to Blame: A Theoretically-Informed Study of Stigma of Hoarding Disorder and Obsessive-Compulsive Disorder
Creator
Chasson, Gregory S., Guy, Arryn A., Bates, Sage, Corrigan, Patrick W.
Date
2018, 2018
Publisher
Elsevier
Machine Learning at the Bureau of Labor Statistics
Title
Machine Learning at the Bureau of Labor Statistics
Creator
Ellis, Robert, Kannan, Vinesh
Date
2019-11-21
Description
Vinesh Kannan (CS '19) shares his experiences working as a...
Show moreVinesh Kannan (CS '19) shares his experiences working as a data science fellow at the Bureau of Labor Statistics (BLS). Vinesh worked on the team that produces occupation and wage data used by policymakers, hiring staff, job seekers, and researchers across the country. He helped improve machine learning systems at the BLS: automatically identifying problematic training data and classifying rare jobs. Vinesh offers advice for students who may be interested in applying for the 2020 Civic Digital Fellowship, a program that recruits university students at all levels to spend a summer working on civic technology projects with various federal agencies.
Sponsorship: College of Science, Department of Computer Science, Department of Applied Mathematics, Machine Learning at IIT
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Professionalism Among Chinese Engineers: An Empirical Study
Title
Professionalism Among Chinese Engineers: An Empirical Study
Creator
Wei, Lina, Davis, Michael
Date
2019
Description
Spreadsheet of answers from a questionnaire sent out in 2018-2019 to Chinese engineering students looking at their perceptions of professional...
Show moreSpreadsheet of answers from a questionnaire sent out in 2018-2019 to Chinese engineering students looking at their perceptions of professional and engineering ethics.
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Collection
Center for the Study of Ethics in the Collection - "China's Unwritten Code of Ethics"
China's Unwritten Code of Engineering Ethics. English Final Data Set
Title
China's Unwritten Code of Engineering Ethics. English Final Data Set
Creator
Wei, Lina, Davis, Michael
Date
2020, 2020
Publisher
Springer
Description
This dataset contains the final results of a survey completed by several hundred engineers in China about what they think about engineering...
Show moreThis dataset contains the final results of a survey completed by several hundred engineers in China about what they think about engineering ethics, their awareness of ethics in their work, and how Chinese engineers' view of engineering ethics is not very different from those of American Engineers.
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Optimizing Cell-Free Protein Synthesis for Increased Yield and Activity of Colicins
Title
Optimizing Cell-Free Protein Synthesis for Increased Yield and Activity of Colicins
Creator
Jin, Xing, Kightlinger, Weston, Hong, Seok Hoon
Date
2019
Publisher
MDPI
Description
Colicins are antimicrobial proteins produced by Escherichia coli that hold great promise as viable complements or alternatives to antibiotics....
Show moreColicins are antimicrobial proteins produced by Escherichia coli that hold great promise as viable complements or alternatives to antibiotics. Cell-free protein synthesis (CFPS) is a useful production platform for toxic proteins because it eliminates the need to maintain cell viability, a common problem in cell-based production. Previously, we demonstrated that colicins produced by CFPS based on crude Escherichia coli lysates are e?ective in eradicating antibiotic-tolerant bacteria known as persisters. However, we also found that some colicins have poor solubility or low cell-killing activity. In this study, we improved the solubility of colicin M from 16% to nearly 100% by producing it in chaperone-enriched E. coli extracts, resulting in enhanced cell-killing activity. We also improved the cytotoxicity of colicin E3 by adding or co-expressing the E3 immunity protein during the CFPS reaction, suggesting that the E3 immunity protein enhances colicin E3 activity in addition to protecting the host strain. Finally, we con?rmed our previous ?nding that active colicins can be rapidly synthesized by observing colicin E1 production over time in CFPS. Within three hours of CFPS incubation, colicin E1 reached its maximum production yield and maintained high cytotoxicity during longer incubations up to 20 h. Taken together, our ?ndings indicate that colicin production can be easily optimized for improved solubility and activity using the CFPS platform.
Sponsorship: NIH R15AI130988
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Controlling biofilms using synthetic biology approaches
Title
Controlling biofilms using synthetic biology approaches
Creator
Fang, Kuili, Park, Oh-Jin, Hong, Seok Hoon
Date
2020
Description
Bacterial biofilms are formed by the complex but ordered regulation of intra- or inter-cellular communication, environmentally responsive gene...
Show moreBacterial biofilms are formed by the complex but ordered regulation of intra- or inter-cellular communication, environmentally responsive gene expression, and secretion of extracellular polymeric substances. Given the robust nature of bio?lms due to the non-growing nature of bio?lm bacteria and the physical barrier provided by the extracellular matrix, eradicating bio?lms is a very di?cult task to accomplish with conventional antibiotic or disinfectant treatments. Synthetic biology holds substantial promise for controlling bio?lms by improving and expanding existing biological tools, introducing novel functions to the system, and re-conceptualizing gene regulation. This review summarizes synthetic biology approaches used to eradicate bio?lms via protein engineering of bio?lm-related enzymes, utilization of synthetic genetic circuits, and the development of functional living agents. Synthetic biology also enables bene?cial applications of bio?lms through the production of biomaterials and patterning bio?lms with speci?c temporal and spatial structures. Advances in synthetic biology will add novel bio?lm functionalities for future therapeutic, biomanufacturing, and environmental applications.
Sponsorship: NIH-R15AI130988, NSF CBET-1917130
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Engineering Escherichia coli to produce and secrete colicins for rapid and selective biofilm cell killing
Title
Engineering Escherichia coli to produce and secrete colicins for rapid and selective biofilm cell killing
Creator
Jin, Xing, An, Sungjun, Kightlinger, Weston, Zhou, Jiacheng, Hong, Seok Hoon
Date
2021
Description
Bacterial biofilms are associated with chronic infectious diseases and are highly resistant to conventional antibiotics. Antimicrobial...
Show moreBacterial biofilms are associated with chronic infectious diseases and are highly resistant to conventional antibiotics. Antimicrobial bacteriocins are alternatives to conventional antibiotics and are characterized by unique cell-killing mechanisms, including pore formation on cell membranes, nuclease activity, and cell wall synthesis inhibition. Here, we used cell-free protein synthesis to rapidly evaluate the antibiofilm activities of colicins E1, E2, and E3. We found that E2 (with DNase activity) most effectively killed target biofilm cells (i.e., the K361 strain) while leaving nontargeted biofilms intact. We then engineered probiotic Escherichia coli microorganisms with genetic circuits to controllably synthesize and secrete colicin E2, which successfully inhibited biofilms and killed preformed indicator biofilms. Our findings suggest that colicins rapidly and selectively kill target biofilm cells in multispecies biofilms and demonstrate the potential of using microorganisms engineered to produce antimicrobial colicin proteins as live therapeutic strategies to treat biofilm-associated infections.
Sponsorship: NIH-R15AI130988
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Editorial, "Cell-Free Synthetic Biology": Synthetic Biology Meets Cell-Free Protein Synthesis
Title
Editorial, "Cell-Free Synthetic Biology": Synthetic Biology Meets Cell-Free Protein Synthesis
Creator
Hong, Seok Hoon
Date
2019
Publisher
MDPI
Description
Sponsorship: NIH R15AI130988
Incorporation of non-standard amino acids into proteins: challenges, recent achievements, and emerging applications
Title
Incorporation of non-standard amino acids into proteins: challenges, recent achievements, and emerging applications
Creator
Jin, Xing, Park Oh-Jin, Hong, Seok Hoon
Date
2019
Description
The natural genetic code only allows for 20 standard amino acids in protein translation, but genetic code reprogramming enables the...
Show moreThe natural genetic code only allows for 20 standard amino acids in protein translation, but genetic code reprogramming enables the incorporation of non-standard amino acids (NSAAs). Proteins containing NSAAs provide enhanced or novel properties and open diverse applications. With increased attention to the recent advancements in synthetic biology, various improved and novel methods have been developed to incorporate single and multiple distinct NSAAs into proteins. However, various challenges remain in regard to NSAA incorporation, such as low yield and misincorporation. In this review, we summarize the recent efforts to improve NSAA incorporation by utilizing orthogonal translational system optimization, cell-free protein synthesis, genomically recoded organisms, artificial codon boxes, quadruplet codons, and orthogonal ribosomes, before closing with a discussion of the emerging applications of NSAA incorporation.
Sponsorship: NIH R15AI130988
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Undecanoic Acid, Lauric Acid, and N-Tridecanoic Acid Inhibit Escherichia coli Persistence and Biofilm Formation
Title
Undecanoic Acid, Lauric Acid, and N-Tridecanoic Acid Inhibit Escherichia coli Persistence and Biofilm Formation
Creator
Jin, Xing, Zhou, Jiacheng, Richey, Gabriella, Wang, Mengya, Hong, Sung Min Choi, Hong, Seok Hoon
Date
2021
Description
Persister cell formation and biofilms of pathogens are extensively involved in the development of chronic infectious diseases. Eradicating...
Show morePersister cell formation and biofilms of pathogens are extensively involved in the development of chronic infectious diseases. Eradicating persister cells is challenging, owing to their tolerance to conventional antibiotics, which cannot kill cells in a metabolically dormant state. A high frequency of persisters in biofilms makes inactivating biofilm cells more difficult, because the biofilm matrix inhibits antibiotic penetration. Fatty acids may be promising candidates as antipersister or antibiofilm agents, because some fatty acids exhibit antimicrobial effects. We previously reported that fatty acid ethyl esters effectively inhibit Escherichia coli persister formation by regulating an antitoxin. In this study, we screened a fatty acid library consisting of 65 different fatty acid molecules for altered persister formation. We found that undecanoic acid, lauric acid, and N-tridecanoic acid inhibited E. coli BW25113 persister cell formation by 25-, 58-, and 44-fold, respectively. Similarly, these fatty acids repressed persisters of enterohemorrhagic E. coli EDL933. These fatty acids were all medium-chain saturated forms. Furthermore, the fatty acids repressed Enterohemorrhagic E. coli (EHEC) biofilm formation (for example, by 8-fold for lauric acid) without having antimicrobial activity. This study demonstrates that medium-chain saturated fatty acids can serve as antipersister and antibiofilm agents that may be applied to treat bacterial infections.
Sponsorship: NIH-R15AI130988; NSF REU-1757989
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Priming Movie Product Placements: New Insights from a Cross-National Case Study
Title
Priming Movie Product Placements: New Insights from a Cross-National Case Study
Creator
Balasubramanian,Siva K, Gistri, Giacomo
Date
44354, 44327
Publisher
Taylor & Francis
Description
The paucity of research on priming product placements and insights from practitioners (Study 1) motivated our investigation into how and when...
Show moreThe paucity of research on priming product placements and insights from practitioners (Study 1) motivated our investigation into how and when priming works in movie placements. Study 2 explores the impact of media priming (a media story announcing a movie placement before the movie’s release) and ad priming (a similar ad announcement) on recall through contrasts with no priming (control). US students watched a movie in a theatre after such priming for a subtle or a prominent placement. When compared to no priming, both media priming and ad priming enhanced recall for the subtle placement; no difference in recall performance emerged between no priming and either type of priming for the prominent placement. Contrast tests comparing media priming and ad priming indicated no differences in recall for either subtle or prominent placement. Study 3 replicated these recall findings with Italian moviegoers, and supported additional hypotheses and propositions for brand attitude. For the subtle (prominent) placement, attitude did not change (decreased) when comparing either media priming or ad priming with no priming. Contrast tests comparing media priming and ad priming indicated no differences in attitude outcomes, for either subtle or prominent placement. Using no priming as a baseline for comparison, the converging conclusion is that any type of priming improves (does not change) recall and does not change (worsens) attitude for subtle (prominent) placements. Overall, results do not support priming for prominent placements; selective use of any type of priming for subtle placements appears appropriate to improve recall outcomes.
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Collection
International Journal of Advertising
Research in Architectural Education: Theory and Practice of Visual Training
Title
Research in Architectural Education: Theory and Practice of Visual Training
Creator
Jones, Kristin
Date
2016, 12/12/2016
Collection
Enquiry