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Title: Engineering Escherichia coli to produce and secrete colicins for rapid and selective biofilm cell killing
Creator: Jin, Xing, An, Sungjun, Kightlinger, Weston, Zhou, Jiacheng, Hong, Seok Hoon
Date: 2021
Description: Bacterial biofilms are associated with chronic infectious diseases and are highly resistant to conventional antibiotics. Antimicrobial...
Show moreBacterial biofilms are associated with chronic infectious diseases and are highly resistant to conventional antibiotics. Antimicrobial bacteriocins are alternatives to conventional antibiotics and are characterized by unique cell-killing mechanisms, including pore formation on cell membranes, nuclease activity, and cell wall synthesis inhibition. Here, we used cell-free protein synthesis to rapidly evaluate the antibiofilm activities of colicins E1, E2, and E3. We found that E2 (with DNase activity) most effectively killed target biofilm cells (i.e., the K361 strain) while leaving nontargeted biofilms intact. We then engineered probiotic Escherichia coli microorganisms with genetic circuits to controllably synthesize and secrete colicin E2, which successfully inhibited biofilms and killed preformed indicator biofilms. Our findings suggest that colicins rapidly and selectively kill target biofilm cells in multispecies biofilms and demonstrate the potential of using microorganisms engineered to produce antimicrobial colicin proteins as live therapeutic strategies to treat biofilm-associated infections.
Sponsorship: NIH-R15AI130988
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Title: Continuous Generalization of 2’s Complement Arithmetic
Creator: Patel, Shivam
Date: 2022-11-26

Title: Editorial, "Cell-Free Synthetic Biology": Synthetic Biology Meets Cell-Free Protein Synthesis
Creator: Hong, Seok Hoon
Date: 2019
Publisher: MDPI
Description: Sponsorship: NIH R15AI130988

Title: Incorporation of non-standard amino acids into proteins: challenges, recent achievements, and emerging applications
Creator: Jin, Xing, Park Oh-Jin, Hong, Seok Hoon
Date: 2019
Description: The natural genetic code only allows for 20 standard amino acids in protein translation, but genetic code reprogramming enables the...
Show moreThe natural genetic code only allows for 20 standard amino acids in protein translation, but genetic code reprogramming enables the incorporation of non-standard amino acids (NSAAs). Proteins containing NSAAs provide enhanced or novel properties and open diverse applications. With increased attention to the recent advancements in synthetic biology, various improved and novel methods have been developed to incorporate single and multiple distinct NSAAs into proteins. However, various challenges remain in regard to NSAA incorporation, such as low yield and misincorporation. In this review, we summarize the recent efforts to improve NSAA incorporation by utilizing orthogonal translational system optimization, cell-free protein synthesis, genomically recoded organisms, artificial codon boxes, quadruplet codons, and orthogonal ribosomes, before closing with a discussion of the emerging applications of NSAA incorporation.
Sponsorship: NIH R15AI130988
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Title: Undecanoic Acid, Lauric Acid, and N-Tridecanoic Acid Inhibit Escherichia coli Persistence and Biofilm Formation
Creator: Jin, Xing, Zhou, Jiacheng, Richey, Gabriella, Wang, Mengya, Hong, Sung Min Choi, Hong, Seok Hoon
Date: 2021
Description: Persister cell formation and biofilms of pathogens are extensively involved in the development of chronic infectious diseases. Eradicating...
Show morePersister cell formation and biofilms of pathogens are extensively involved in the development of chronic infectious diseases. Eradicating persister cells is challenging, owing to their tolerance to conventional antibiotics, which cannot kill cells in a metabolically dormant state. A high frequency of persisters in biofilms makes inactivating biofilm cells more difficult, because the biofilm matrix inhibits antibiotic penetration. Fatty acids may be promising candidates as antipersister or antibiofilm agents, because some fatty acids exhibit antimicrobial effects. We previously reported that fatty acid ethyl esters effectively inhibit Escherichia coli persister formation by regulating an antitoxin. In this study, we screened a fatty acid library consisting of 65 different fatty acid molecules for altered persister formation. We found that undecanoic acid, lauric acid, and N-tridecanoic acid inhibited E. coli BW25113 persister cell formation by 25-, 58-, and 44-fold, respectively. Similarly, these fatty acids repressed persisters of enterohemorrhagic E. coli EDL933. These fatty acids were all medium-chain saturated forms. Furthermore, the fatty acids repressed Enterohemorrhagic E. coli (EHEC) biofilm formation (for example, by 8-fold for lauric acid) without having antimicrobial activity. This study demonstrates that medium-chain saturated fatty acids can serve as antipersister and antibiofilm agents that may be applied to treat bacterial infections.
Sponsorship: NIH-R15AI130988; NSF REU-1757989
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Title: Priming Movie Product Placements: New Insights from a Cross-National Case Study
Creator: Balasubramanian,Siva K, Gistri, Giacomo
Date: 44354, 44327
Publisher: Taylor & Francis
Description: The paucity of research on priming product placements and insights from practitioners (Study 1) motivated our investigation into how and when...
Show moreThe paucity of research on priming product placements and insights from practitioners (Study 1) motivated our investigation into how and when priming works in movie placements. Study 2 explores the impact of media priming (a media story announcing a movie placement before the movie’s release) and ad priming (a similar ad announcement) on recall through contrasts with no priming (control). US students watched a movie in a theatre after such priming for a subtle or a prominent placement. When compared to no priming, both media priming and ad priming enhanced recall for the subtle placement; no difference in recall performance emerged between no priming and either type of priming for the prominent placement. Contrast tests comparing media priming and ad priming indicated no differences in recall for either subtle or prominent placement. Study 3 replicated these recall findings with Italian moviegoers, and supported additional hypotheses and propositions for brand attitude. For the subtle (prominent) placement, attitude did not change (decreased) when comparing either media priming or ad priming with no priming. Contrast tests comparing media priming and ad priming indicated no differences in attitude outcomes, for either subtle or prominent placement. Using no priming as a baseline for comparison, the converging conclusion is that any type of priming improves (does not change) recall and does not change (worsens) attitude for subtle (prominent) placements. Overall, results do not support priming for prominent placements; selective use of any type of priming for subtle placements appears appropriate to improve recall outcomes.
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Collection: International Journal of Advertising

Title: Abraham Lincoln School Art Classes leaflet, 1945-1946
Creator: Abraham Lincoln School
Date: 1945-1946
Description: Course catalog for evening classes in art and design at Abraham Lincoln School (30 West Washington Street, Chicago, Illinois). Class topics...
Show moreCourse catalog for evening classes in art and design at Abraham Lincoln School (30 West Washington Street, Chicago, Illinois). Class topics were layout and typography, basic design, home planning, and photography, and the classes were taught, at least in part, by students from the Institute of Design, including Mary Henry, Morton Goldsholl, and Jack Waldheim. This catalog is Mary Henry's personal copy, and her inscription on the rear cover reads "While I was a student at the Institute of Design I taught one semester at the Abraham Lincoln School - no salary involved! This is my only copy. M.H." Date of leaflet is unknown, date range indicated is inferred from Mary Henry's dates of attendance at the Institute of Design.
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Collection: Mary Dill Henry Papers, 1913-2021

Title: Integral, 1899
Creator: Armour Institute of Technology
Date: 1899
Description: Volume 2 of Integral, the 1899 yearbook for the Armour Institute of Technology. The Armour Institute of Technology yearbooks was known by...
Show moreVolume 2 of Integral, the 1899 yearbook for the Armour Institute of Technology. The Armour Institute of Technology yearbooks was known by three different titles: Integral (1898-1910), Senior Class Book (1911-1912), and The Cycle (1913-1940). A 1902 volume of Integral is not a part of Galvin Library collections; it is uncertain of a yearbook was published that year. Publication of the Armour Institute ceased in 1941, when the Armour Institute of Technology merged with the Lewis Institute to form Illinois Institute of Technology.
Sponsorship: Scanning of the Armour Institute of Technology yearbooks was supported in part by an award from the Illinois State Historical Records Advisory Board, through funding from the National Historical Publications and Records Commission, National Archives and Records Administration.
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Collection: Armour Institute of Technology yearbooks, 1898-1940

Title: Integral, 1900
Creator: Armour Institute of Technology
Date: 1900
Description: Volume 3 of Integral, the 1900 yearbook for the Armour Institute of Technology. The Armour Institute of Technology yearbooks was known by...
Show moreVolume 3 of Integral, the 1900 yearbook for the Armour Institute of Technology. The Armour Institute of Technology yearbooks was known by three different titles: Integral (1898-1910), Senior Class Book (1911-1912), and The Cycle (1913-1940). A 1902 volume of Integral is not a part of Galvin Library collections; it is uncertain of a yearbook was published that year. Publication of the Armour Institute ceased in 1941, when the Armour Institute of Technology merged with the Lewis Institute to form Illinois Institute of Technology.
Sponsorship: Scanning of the Armour Institute of Technology yearbooks was supported in part by an award from the Illinois State Historical Records Advisory Board, through funding from the National Historical Publications and Records Commission, National Archives and Records Administration.
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Collection: Armour Institute of Technology yearbooks, 1898-1940

Title: Integral, 1901
Creator: Armour Institute of Technology
Date: 1901
Description: Volume 4 of Integral, the 1901 yearbook for the Armour Institute of Technology. The Armour Institute of Technology yearbooks was known by...
Show moreVolume 4 of Integral, the 1901 yearbook for the Armour Institute of Technology. The Armour Institute of Technology yearbooks was known by three different titles: Integral (1898-1910), Senior Class Book (1911-1912), and The Cycle (1913-1940). A 1902 volume of Integral is not a part of Galvin Library collections; it is uncertain of a yearbook was published that year. Publication of the Armour Institute ceased in 1941, when the Armour Institute of Technology merged with the Lewis Institute to form Illinois Institute of Technology.
Sponsorship: Scanning of the Armour Institute of Technology yearbooks was supported in part by an award from the Illinois State Historical Records Advisory Board, through funding from the National Historical Publications and Records Commission, National Archives and Records Administration.
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Collection: Armour Institute of Technology yearbooks, 1898-1940

Title: Integral, 1903
Creator: Armour Institute of Technology
Date: 1903
Description: Volume 5 of Integral, the 1903 yearbook for the Armour Institute of Technology. The Armour Institute of Technology yearbooks was known by...
Show moreVolume 5 of Integral, the 1903 yearbook for the Armour Institute of Technology. The Armour Institute of Technology yearbooks was known by three different titles: Integral (1898-1910), Senior Class Book (1911-1912), and The Cycle (1913-1940). A 1902 volume of Integral is not a part of Galvin Library collections; it is uncertain of a yearbook was published that year. Publication of the Armour Institute ceased in 1941, when the Armour Institute of Technology merged with the Lewis Institute to form Illinois Institute of Technology.
Sponsorship: Scanning of the Armour Institute of Technology yearbooks was supported in part by an award from the Illinois State Historical Records Advisory Board, through funding from the National Historical Publications and Records Commission, National Archives and Records Administration.
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Collection: Armour Institute of Technology yearbooks, 1898-1940

Title: The Landings in North Africa November 1942
Creator: Moran, Charles
Date: 1993, 1993
Publisher: Naval Historical Center, Dept. of the Navy,

Title: Student protest at Illinois Institute of Technology, 1970
Creator: Illinois Institute of Technology
Date: 1970
Description: Photograph of Illinois Institute of Technology faculty and students attending an open meeting with President John T. Rettaliata regarding...
Show morePhotograph of Illinois Institute of Technology faculty and students attending an open meeting with President John T. Rettaliata regarding student protests that occurred on IIT's campus following the fatal shooting of four students at Kent State University by the Ohio National Guard on May 4, 1970.
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Collection: John T. Rettaliata student protest photographs, 1970

Title: Student protest at Illinois Institute of Technology, 1970
Creator: Illinois Institute of Technology
Date: 1970
Description: Photograph of student protests outside of Perlstein Hall on Illinois Institute of Technology's campus following the fatal shooting of four...
Show morePhotograph of student protests outside of Perlstein Hall on Illinois Institute of Technology's campus following the fatal shooting of four students at Kent State University by the Ohio National Guard on May 4, 1970.
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Collection: John T. Rettaliata student protest photographs, 1970

Title: Student protest at Illinois Institute of Technology, 1970
Creator: Illinois Institute of Technology
Date: 1970
Description: Photograph of student protests outside of Perlstein Hall on Illinois Institute of Technology's campus following the fatal shooting of four...
Show morePhotograph of student protests outside of Perlstein Hall on Illinois Institute of Technology's campus following the fatal shooting of four students at Kent State University by the Ohio National Guard on May 4, 1970.
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Collection: John T. Rettaliata student protest photographs, 1970

Title: Student protest at Illinois Institute of Technology, 1970
Creator: Illinois Institute of Technology
Date: 1970
Description: Photograph of student protesters gathered outside of President John T. Rettaliata's office in Perlstein Hall on Illinois Institute of...
Show morePhotograph of student protesters gathered outside of President John T. Rettaliata's office in Perlstein Hall on Illinois Institute of Technology's campus. Student protests took place following the fatal shooting of four students at Kent State University by the Ohio National Guard on May 4, 1970.
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Collection: John T. Rettaliata student protest photographs, 1970

Title: Student protest at Illinois Institute of Technology, 1970
Creator: Illinois Institute of Technology
Date: 1970
Description: Photograph of student protesters gathered outside of President John T. Rettaliata's office in Perlstein Hall on Illinois Institute of...
Show morePhotograph of student protesters gathered outside of President John T. Rettaliata's office in Perlstein Hall on Illinois Institute of Technology's campus. Student protests took place following the fatal shooting of four students at Kent State University by the Ohio National Guard on May 4, 1970.
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Collection: John T. Rettaliata student protest photographs, 1970

Title: Benny Goodman receiving the IIT Hall of Fame medal and plaque from IIT Provost Sidney Guralnick, Chicago, Illinois, ca. 1980
Date: 1980
Description: Photograph of Provost Sidney Guralnick presenting the IIT Hall of Fame medal and plaque to Benny Goodman. Goodman attended Lewis Institute in...
Show morePhotograph of Provost Sidney Guralnick presenting the IIT Hall of Fame medal and plaque to Benny Goodman. Goodman attended Lewis Institute in 1923 as a high school sophomore while also playing the clarinet in a dance hall band. The legendary orchestra conductor and jazz musician received an honorary LL.D. degree from IIT in 1968. Photographer unknown. Date listed is approximate.
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Collection: Office of Communications and Marketing photographs, 1905-1999

Title: Student protest at Illinois Institute of Technology, 1970
Creator: Illinois Institute of Technology
Date: 1970
Description: Photograph of student protests outside of Perlstein Hall on Illinois Institute of Technology's campus following the fatal shooting of four...
Show morePhotograph of student protests outside of Perlstein Hall on Illinois Institute of Technology's campus following the fatal shooting of four students at Kent State University by the Ohio National Guard on May 4, 1970.
Show less
Collection: John T. Rettaliata student protest photographs, 1970

Title: Student protest at Illinois Institute of Technology, 1970
Creator: Illinois Institute of Technology
Date: 1970
Description: Photograph of Illinois Institute of Technology faculty and students attending an open meeting with IIT President John Rettaliata regarding...
Show morePhotograph of Illinois Institute of Technology faculty and students attending an open meeting with IIT President John Rettaliata regarding student protests that occurred on IIT's campus following the fatal shooting of four students at Kent State University by the Ohio National Guard on May 4, 1970.
Show less
Collection: John T. Rettaliata student protest photographs, 1970

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