Ask a lawyer what "professionalism" means and you are likely to hear that professionalism means putting your client first or acting as an... Show moreAsk a lawyer what "professionalism" means and you are likely to hear that professionalism means putting your client first or acting as an officer of the court. Only rarely will a lawyer say that professionalism means putting justice first. Never, I think, will a lawyer even suggest that professionalism means putting your profession first. Yet this is the thesis of this paper. The paper has three parts. Section I makes certain distinctions necessary to prevent misunderstanding my thesis. Section II and III develop the thesis into a conception of professionalism. Sections IV and V use that conception to help with the most difficult of undertakings, justifying professional discipline to someone convicted of professional misconduct which harmed neither her client nor an identifiable third party. Georgetown Journal of Legal Ethics. Vol. 2, Issue 1. Summer 1988. pp.341-357. Show less
Experimental Design and Analysis textbook originally written for use in 36-309/749, Experimental Design for Behavioral and Social Sciences at... Show moreExperimental Design and Analysis textbook originally written for use in 36-309/749, Experimental Design for Behavioral and Social Sciences at Carnegie Mellon University. Show less
Colicins are antimicrobial proteins produced by Escherichia coli that hold great promise as viable complements or alternatives to antibiotics.... Show moreColicins are antimicrobial proteins produced by Escherichia coli that hold great promise as viable complements or alternatives to antibiotics. Cell-free protein synthesis (CFPS) is a useful production platform for toxic proteins because it eliminates the need to maintain cell viability, a common problem in cell-based production. Previously, we demonstrated that colicins produced by CFPS based on crude Escherichia coli lysates are e?ective in eradicating antibiotic-tolerant bacteria known as persisters. However, we also found that some colicins have poor solubility or low cell-killing activity. In this study, we improved the solubility of colicin M from 16% to nearly 100% by producing it in chaperone-enriched E. coli extracts, resulting in enhanced cell-killing activity. We also improved the cytotoxicity of colicin E3 by adding or co-expressing the E3 immunity protein during the CFPS reaction, suggesting that the E3 immunity protein enhances colicin E3 activity in addition to protecting the host strain. Finally, we con?rmed our previous ?nding that active colicins can be rapidly synthesized by observing colicin E1 production over time in CFPS. Within three hours of CFPS incubation, colicin E1 reached its maximum production yield and maintained high cytotoxicity during longer incubations up to 20 h. Taken together, our ?ndings indicate that colicin production can be easily optimized for improved solubility and activity using the CFPS platform. Sponsorship: NIH R15AI130988 Show less
Photograph of an unidentified Chicago School of Design or Institute of Design student applying what appears to be plaster to a constructed... Show morePhotograph of an unidentified Chicago School of Design or Institute of Design student applying what appears to be plaster to a constructed form. Photographer unknown. Date of photograph unknown. Date listed is approximate. Show less
Experimental Design and Analysis textbook originally written for use in 36-309/749, Experimental Design for Behavioral and Social Sciences at... Show moreExperimental Design and Analysis textbook originally written for use in 36-309/749, Experimental Design for Behavioral and Social Sciences at Carnegie Mellon University. Show less
Flyer advertising available leases for Gunsaulus Hall apartments, posted in May 1952. Gunsaulus Hall was designed by Skidmore, Owings &... Show moreFlyer advertising available leases for Gunsaulus Hall apartments, posted in May 1952. Gunsaulus Hall was designed by Skidmore, Owings & Merrill and was constructed in 1949-1950. Show less
Booklet detailing apartment/dormitory housing options available to Illinois Tech students, faculty, and staff. Published soon after the... Show moreBooklet detailing apartment/dormitory housing options available to Illinois Tech students, faculty, and staff. Published soon after the construction of Cunningham Hall and Bailey Hall (later George J. Kacek Hall), the booklet includes descriptions of different apartment layouts and floor plans for each building. Date unknown. Date listed is approximate, based on campus layout depicted in booklet and mention of Mies van der Rohe as current director of the Department of Architecture. Show less
Photograph of Robert Arzbaecher and Bob Jaeger of the Pritzker Institute of Medical Engineering (now Pritzker Institute of Biomedical Science... Show morePhotograph of Robert Arzbaecher and Bob Jaeger of the Pritzker Institute of Medical Engineering (now Pritzker Institute of Biomedical Science and Engineering). Photographer unknown. Date of photograph unknown. Date range listed is approximate. Show less
Photograph of Robert Arzbaecher of the Pritzker Institute of Medical Engineering (now Pritzker Institute of Biomedical Science and... Show morePhotograph of Robert Arzbaecher of the Pritzker Institute of Medical Engineering (now Pritzker Institute of Biomedical Science and Engineering) holding a smart pacemaker, potentially an external pacemaker used in conjunction with the swallowable pill electrode Arzbaecher developed for use in the detection and analysis of cardiac arrythmia. Photographer unknown. Date of photograph unknown. Date range listed is approximate. Show less
Photograph of an implantable drug pump for treating cardiac arrhythmia developed by Robert Arzbaecher at the Pritzker Institute of Medical... Show morePhotograph of an implantable drug pump for treating cardiac arrhythmia developed by Robert Arzbaecher at the Pritzker Institute of Medical Engineering (now Pritzker Institute of Biomedical Science and Engineering). Photographer unknown. Date of photograph unknown. Date range listed is approximate. Show less
Photograph of a swallowable pill electrode used in the detection and analysis of cardiac arrythmia, including an Aspirin tablet for size... Show morePhotograph of a swallowable pill electrode used in the detection and analysis of cardiac arrythmia, including an Aspirin tablet for size comparison. The pill electrode was developed by Robert Arzbaecher at the Pritzker Institute of Medical Engineering (now Pritzker Institute of Biomedical Science and Engineering). Photographer unknown. Show less
Picture of 1899 Lewis Institute faculty clipped from a newspaper in the 1920s. Those pictured include George Noble Carman (Director of Lewis... Show morePicture of 1899 Lewis Institute faculty clipped from a newspaper in the 1920s. Those pictured include George Noble Carman (Director of Lewis Institute), John L. Bacon (later Mayor of San Diego, 1921-1927), Wallace W. Atwood (later President of Clark University, 1920-1946). Photographer and original source unknown. Show less
Photograph of cut and folded paper used as a means to experiment with light and shadow. Title, date, and signature inscribed on verso.... Show morePhotograph of cut and folded paper used as a means to experiment with light and shadow. Title, date, and signature inscribed on verso. Numbered img1136, 026.087.3.06, #42.1.2, and 2886? on verso of photograph. Show less
Bacterial biofilms are formed by the complex but ordered regulation of intra- or inter-cellular communication, environmentally responsive gene... Show moreBacterial biofilms are formed by the complex but ordered regulation of intra- or inter-cellular communication, environmentally responsive gene expression, and secretion of extracellular polymeric substances. Given the robust nature of bio?lms due to the non-growing nature of bio?lm bacteria and the physical barrier provided by the extracellular matrix, eradicating bio?lms is a very di?cult task to accomplish with conventional antibiotic or disinfectant treatments. Synthetic biology holds substantial promise for controlling bio?lms by improving and expanding existing biological tools, introducing novel functions to the system, and re-conceptualizing gene regulation. This review summarizes synthetic biology approaches used to eradicate bio?lms via protein engineering of bio?lm-related enzymes, utilization of synthetic genetic circuits, and the development of functional living agents. Synthetic biology also enables bene?cial applications of bio?lms through the production of biomaterials and patterning bio?lms with speci?c temporal and spatial structures. Advances in synthetic biology will add novel bio?lm functionalities for future therapeutic, biomanufacturing, and environmental applications. Sponsorship: NIH-R15AI130988, NSF CBET-1917130 Show less