Search results

Title: Semantics and further Use-Cases and Evaluation of the C-Saw language
Creator: Zhu, Henry, Zhao, Junyong, Sultana, Nik
Date: 2023-03-09
Description: This report provides supplementary technical details to the conference paper that introduced C-Saw, a language for expressing software...
Show moreThis report provides supplementary technical details to the conference paper that introduced C-Saw, a language for expressing software architecture patterns. This report provides additional examples of using C-Saw, supplementary evaluation details, and it defines the formal semantics of the language.
Show less

Title: Northeast corner of 35th Street and State Street, Chicago, Illinois, January 24, 1954
Date: 1954-01-24
Description: Color slide depicting the intersection of 35th Street and South State Street, looking northeast. Photographer unknown, possibly a member of...
Show moreColor slide depicting the intersection of 35th Street and South State Street, looking northeast. Photographer unknown, possibly a member of the university's Department of Public Relations.
Show less
Collection: Dan Ryan collection, 1954-1980

Title: László Moholy-Nagy with hand sculptures, ca. 1945
Date: 1945
Description: Photograph of László Moholy-Nagy with hand sculptures. Photographer unknown. Date of photograph unknown. Date listed is approximate, based on...
Show morePhotograph of László Moholy-Nagy with hand sculptures. Photographer unknown. Date of photograph unknown. Date listed is approximate, based on notation found adjacent to photograph.
Show less
Collection: Institute of Design records, 1937-ca. 1962

Title: László Moholy-Nagy
Description: Photograph of László Moholy-Nagy in profile. Photographer unknown. Date of photograph is unknown.
Collection: Institute of Design records, 1937-ca. 1962

Title: Armour Engineer, Index 1-8, 1909-1916
Creator: Armour Institute of Technology
Date: 1916
Description: General index for volumes 1-8 of the Armour Engineer, a serial publication for Armour Institute alumni with school, research, and engineering...
Show moreGeneral index for volumes 1-8 of the Armour Engineer, a serial publication for Armour Institute alumni with school, research, and engineering news. Index compiled by Ellyn C. Broomell, Assistant Librarian, Armour Institute of Technology.
Show less
Collection: Armour Engineer alumni magazine, 1909-1935

Title: Experimental Design and Analysis
Creator: Seltman, Howard J.
Date: 2018-07-11
Description: Experimental Design and Analysis textbook originally written for use in 36-309/749, Experimental Design for Behavioral and Social Sciences at...
Show moreExperimental Design and Analysis textbook originally written for use in 36-309/749, Experimental Design for Behavioral and Social Sciences at Carnegie Mellon University.
Show less

Title: Experimental Design and Analysis data files
Creator: Seltman, Howard J.
Date: 2018-07-11
Description: Data files for use with the Experimental Design and Analysis textbook.

Title: Optimizing Cell-Free Protein Synthesis for Increased Yield and Activity of Colicins
Creator: Jin, Xing, Kightlinger, Weston, Hong, Seok Hoon
Date: 2019
Publisher: MDPI
Description: Colicins are antimicrobial proteins produced by Escherichia coli that hold great promise as viable complements or alternatives to antibiotics....
Show moreColicins are antimicrobial proteins produced by Escherichia coli that hold great promise as viable complements or alternatives to antibiotics. Cell-free protein synthesis (CFPS) is a useful production platform for toxic proteins because it eliminates the need to maintain cell viability, a common problem in cell-based production. Previously, we demonstrated that colicins produced by CFPS based on crude Escherichia coli lysates are e?ective in eradicating antibiotic-tolerant bacteria known as persisters. However, we also found that some colicins have poor solubility or low cell-killing activity. In this study, we improved the solubility of colicin M from 16% to nearly 100% by producing it in chaperone-enriched E. coli extracts, resulting in enhanced cell-killing activity. We also improved the cytotoxicity of colicin E3 by adding or co-expressing the E3 immunity protein during the CFPS reaction, suggesting that the E3 immunity protein enhances colicin E3 activity in addition to protecting the host strain. Finally, we con?rmed our previous ?nding that active colicins can be rapidly synthesized by observing colicin E1 production over time in CFPS. Within three hours of CFPS incubation, colicin E1 reached its maximum production yield and maintained high cytotoxicity during longer incubations up to 20 h. Taken together, our ?ndings indicate that colicin production can be easily optimized for improved solubility and activity using the CFPS platform.
Sponsorship: NIH R15AI130988
Show less

Title: "China's unwritten code of engineering ethics": Table list (1), China's unwritten code of engineering ethics: Table list (1)
Creator: Davis, Michael, Wei, Lina
Date: 2019-08-02, 2019-08-02

Title: Fostering personal finance engagement with a habit building app
Creator: Hapner, Bridget
Date: 2022-11-04
Description: Master's Project focused on developing a concept for a personal finance literacy app.

Title: Experimental Design and Analysis
Creator: Seltman, Howard J.
Date: 2018-07-11
Description: Experimental Design and Analysis textbook originally written for use in 36-309/749, Experimental Design for Behavioral and Social Sciences at...
Show moreExperimental Design and Analysis textbook originally written for use in 36-309/749, Experimental Design for Behavioral and Social Sciences at Carnegie Mellon University.
Show less

Title: Gunsaulus Hall flyer, 1952
Creator: Illinois Institute of Technology
Date: 1952-05
Description: Flyer advertising available leases for Gunsaulus Hall apartments, posted in May 1952. Gunsaulus Hall was designed by Skidmore, Owings &...
Show moreFlyer advertising available leases for Gunsaulus Hall apartments, posted in May 1952. Gunsaulus Hall was designed by Skidmore, Owings & Merrill and was constructed in 1949-1950.
Show less
Collection: Student Housing brochures, 1948-1970

Title: Apartment Living at Technology Center
Creator: Illinois Institute of Technology
Date: 1955
Description: Booklet detailing apartment/dormitory housing options available to Illinois Tech students, faculty, and staff. Published soon after the...
Show moreBooklet detailing apartment/dormitory housing options available to Illinois Tech students, faculty, and staff. Published soon after the construction of Cunningham Hall and Bailey Hall (later George J. Kacek Hall), the booklet includes descriptions of different apartment layouts and floor plans for each building. Date unknown. Date listed is approximate, based on campus layout depicted in booklet and mention of Mies van der Rohe as current director of the Department of Architecture.
Show less
Collection: Student Housing brochures, 1948-1970

Title: Lewis Institute faculty group portrait, Chicago, Illinois, 1899
Date: 1899
Description: Picture of 1899 Lewis Institute faculty clipped from a newspaper in the 1920s. Those pictured include George Noble Carman (Director of Lewis...
Show morePicture of 1899 Lewis Institute faculty clipped from a newspaper in the 1920s. Those pictured include George Noble Carman (Director of Lewis Institute), John L. Bacon (later Mayor of San Diego, 1921-1927), Wallace W. Atwood (later President of Clark University, 1920-1946). Photographer and original source unknown.
Show less
Collection: Lewis Institute records, 1827-1984

Title: Controlling biofilms using synthetic biology approaches
Creator: Fang, Kuili, Park, Oh-Jin, Hong, Seok Hoon
Date: 2020
Description: Bacterial biofilms are formed by the complex but ordered regulation of intra- or inter-cellular communication, environmentally responsive gene...
Show moreBacterial biofilms are formed by the complex but ordered regulation of intra- or inter-cellular communication, environmentally responsive gene expression, and secretion of extracellular polymeric substances. Given the robust nature of bio?lms due to the non-growing nature of bio?lm bacteria and the physical barrier provided by the extracellular matrix, eradicating bio?lms is a very di?cult task to accomplish with conventional antibiotic or disinfectant treatments. Synthetic biology holds substantial promise for controlling bio?lms by improving and expanding existing biological tools, introducing novel functions to the system, and re-conceptualizing gene regulation. This review summarizes synthetic biology approaches used to eradicate bio?lms via protein engineering of bio?lm-related enzymes, utilization of synthetic genetic circuits, and the development of functional living agents. Synthetic biology also enables bene?cial applications of bio?lms through the production of biomaterials and patterning bio?lms with speci?c temporal and spatial structures. Advances in synthetic biology will add novel bio?lm functionalities for future therapeutic, biomanufacturing, and environmental applications.
Sponsorship: NIH-R15AI130988, NSF CBET-1917130
Show less

Title: Engineering Escherichia coli to produce and secrete colicins for rapid and selective biofilm cell killing
Creator: Jin, Xing, An, Sungjun, Kightlinger, Weston, Zhou, Jiacheng, Hong, Seok Hoon
Date: 2021
Description: Bacterial biofilms are associated with chronic infectious diseases and are highly resistant to conventional antibiotics. Antimicrobial...
Show moreBacterial biofilms are associated with chronic infectious diseases and are highly resistant to conventional antibiotics. Antimicrobial bacteriocins are alternatives to conventional antibiotics and are characterized by unique cell-killing mechanisms, including pore formation on cell membranes, nuclease activity, and cell wall synthesis inhibition. Here, we used cell-free protein synthesis to rapidly evaluate the antibiofilm activities of colicins E1, E2, and E3. We found that E2 (with DNase activity) most effectively killed target biofilm cells (i.e., the K361 strain) while leaving nontargeted biofilms intact. We then engineered probiotic Escherichia coli microorganisms with genetic circuits to controllably synthesize and secrete colicin E2, which successfully inhibited biofilms and killed preformed indicator biofilms. Our findings suggest that colicins rapidly and selectively kill target biofilm cells in multispecies biofilms and demonstrate the potential of using microorganisms engineered to produce antimicrobial colicin proteins as live therapeutic strategies to treat biofilm-associated infections.
Sponsorship: NIH-R15AI130988
Show less

Title: Continuous Generalization of 2’s Complement Arithmetic
Creator: Patel, Shivam
Date: 2022-11-26

Title: Editorial, "Cell-Free Synthetic Biology": Synthetic Biology Meets Cell-Free Protein Synthesis
Creator: Hong, Seok Hoon
Date: 2019
Publisher: MDPI
Description: Sponsorship: NIH R15AI130988

Title: Incorporation of non-standard amino acids into proteins: challenges, recent achievements, and emerging applications
Creator: Jin, Xing, Park Oh-Jin, Hong, Seok Hoon
Date: 2019
Description: The natural genetic code only allows for 20 standard amino acids in protein translation, but genetic code reprogramming enables the...
Show moreThe natural genetic code only allows for 20 standard amino acids in protein translation, but genetic code reprogramming enables the incorporation of non-standard amino acids (NSAAs). Proteins containing NSAAs provide enhanced or novel properties and open diverse applications. With increased attention to the recent advancements in synthetic biology, various improved and novel methods have been developed to incorporate single and multiple distinct NSAAs into proteins. However, various challenges remain in regard to NSAA incorporation, such as low yield and misincorporation. In this review, we summarize the recent efforts to improve NSAA incorporation by utilizing orthogonal translational system optimization, cell-free protein synthesis, genomically recoded organisms, artificial codon boxes, quadruplet codons, and orthogonal ribosomes, before closing with a discussion of the emerging applications of NSAA incorporation.
Sponsorship: NIH R15AI130988
Show less

Title: Undecanoic Acid, Lauric Acid, and N-Tridecanoic Acid Inhibit Escherichia coli Persistence and Biofilm Formation
Creator: Jin, Xing, Zhou, Jiacheng, Richey, Gabriella, Wang, Mengya, Hong, Sung Min Choi, Hong, Seok Hoon
Date: 2021
Description: Persister cell formation and biofilms of pathogens are extensively involved in the development of chronic infectious diseases. Eradicating...
Show morePersister cell formation and biofilms of pathogens are extensively involved in the development of chronic infectious diseases. Eradicating persister cells is challenging, owing to their tolerance to conventional antibiotics, which cannot kill cells in a metabolically dormant state. A high frequency of persisters in biofilms makes inactivating biofilm cells more difficult, because the biofilm matrix inhibits antibiotic penetration. Fatty acids may be promising candidates as antipersister or antibiofilm agents, because some fatty acids exhibit antimicrobial effects. We previously reported that fatty acid ethyl esters effectively inhibit Escherichia coli persister formation by regulating an antitoxin. In this study, we screened a fatty acid library consisting of 65 different fatty acid molecules for altered persister formation. We found that undecanoic acid, lauric acid, and N-tridecanoic acid inhibited E. coli BW25113 persister cell formation by 25-, 58-, and 44-fold, respectively. Similarly, these fatty acids repressed persisters of enterohemorrhagic E. coli EDL933. These fatty acids were all medium-chain saturated forms. Furthermore, the fatty acids repressed Enterohemorrhagic E. coli (EHEC) biofilm formation (for example, by 8-fold for lauric acid) without having antimicrobial activity. This study demonstrates that medium-chain saturated fatty acids can serve as antipersister and antibiofilm agents that may be applied to treat bacterial infections.
Sponsorship: NIH-R15AI130988; NSF REU-1757989
Show less

repository.iit

Search the repository

Pages

Pages

Enabled Filters

Refine Results

Type

Date

Academic status of creator

Department

Subject

Creator

Part of

Rights