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(281 - 300 of 3,952)
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- Title
- Design of Kranti Home and School (Mumbai) for Sexually Trafficked Girls (sequence unknown), IPRO 343 - Project Plan
- Creator
- Arastu, Insiyah, Choe, Jonathan, Davis, Elena, Enriquez, Sheena, Kimball, Mark, Kleehammer, Christopher, Klimek, Joseph, Marcheva, Boryana, Martin, Amy, Patel, Hamida, Patel, Avni, Semerdjiev, Ivo, Sturrup, Terez
- Date
- 2009-12
- Description
-
To develop and design a home and center of education for girls who have been previously trafficked.
Project Plan for IPRO 343: Design of Kranti...
Show moreTo develop and design a home and center of education for girls who have been previously trafficked.
Project Plan for IPRO 343: Design of Kranti Home and School (Mumbai) for Sexually Trafficked Girls for the fall 2009 semester
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- Title
- FUNCTIONAL ANALYSIS OF POTENTIAL PHOSPHORYLATION SITES IN BAXΔ2 UNIQUE OLIGOPEPTIDE
- Creator
- Tsai, Yu-tseng
- Date
- 2014, 2014-07
- Description
-
The tumor suppressor gene, Bax, plays a critical role in tumor progression through regulating cell apoptosis. Mutations on the BAX gene often...
Show moreThe tumor suppressor gene, Bax, plays a critical role in tumor progression through regulating cell apoptosis. Mutations on the BAX gene often result in silencing its expression and the loss of pro-death ability. However, there is a unique Bax isoform, BaxΔ2, recently discovered in these Bax mutated cancer cells. BaxΔ2 isoform shows higher pro-apoptotic activity than Baxα. Unlike the parental Baxα, BaxΔ2 does not target mitochondria and forms aggregates in cytosol. There is a unique 10-amino-acid peptide in the N-terminus of BaxΔ2 protein possible function as a special signal. Two serines in this region are predicted as potential phosphorylation sites for regulation of the protein activity. To test this hypothesis, we mutated both serines (SS) into non-phosphorylatable alanines (AA) by site-directed mutagenesis approach. Both BaxΔ2 wild type (BaxΔ2-SS) and mutants (BaxΔ2-AA) were tagged with GFP, which allows us to monitor the protein expression and cellular localization in live cells. Here, we found that the distribution patterns of BaxΔ2-AA and BaxΔ2-SS were similar and appeared as aggregates in cytosol. BaxΔ2-AA mutant also possessed the similar pro-apoptotic activity with BaxΔ2-SS wild type. These results suggested that the two serines in BaxΔ2 unique oligopeptide might not play a critical role in BaxΔ2 localization and pro-death activity under the current ectopic expression conditions. Further study is needed to have better understanding of phosphorylation in contribution to unique behavior of BaxΔ2.
M.S. in Biology, July 2014
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- Title
- The utilization of sunflower stalks in paper manufacture
- Creator
- Zavertnik, J.
- Date
- 2009, 1914
- Publisher
- Armour Institute of Technology
- Description
-
http://www.archive.org/details/utilizationofsun00zave
Thesis (B.S.)--Armour Institute of Technology
- Title
- POINT CLOUD FUSION BETWEEEN AERIAL AND VEHICLE LIDAR
- Creator
- Guangyao, Ma
- Date
- 2015, 2015-05
- Description
-
Because of the increasing requirement of precision in region of 3-D map, we began to use LiDAR to establish a more accurate map. There still...
Show moreBecause of the increasing requirement of precision in region of 3-D map, we began to use LiDAR to establish a more accurate map. There still exist some problems although we have already made a great progress in this area. One of them, which I tried to process during my thesis study, is that we have two points source - Aerial LiDAR Data( Points gotten by Airplane ) and Vehicle LiDAR Data( Points gotten by Vehicle ) - while both of them have a different density and cannot be merged well. This process - Fusion-is kindly similar to registration, the difference is that the points we would like to merge are generated from different devices and have only few points pairs in the same region. For example, the Aerial LiDAR data has a higher points density in the roofs and ground, but lower in the walls. In the meanwhile, the Vehicle LiDAR data has a lot of points in the walls and ground region. It is beneficial to minimize the difference between these two point sets since the process is necessary for modeling, registration and so on. Therefore, my thesis is to minimize the difference between these two data sources, a procedure of Fusion. The main idea is to read the LiDAR data into data structure of Point Cloud, sample their density to the similar level, and select several corresponding special region pairs( we named these regions -chunks, e.g. Median strip and boundaries of road ) with sufficient interesting points to do fusion. Interesting points indicate the points with one and more special features among all points. And, the algorithm we used to implement the fusion is ICP( Iterative Closet Point Algorithm). Not similar to Registration of Point Cloud, research in the Fusion area is rare. Therefore, the existing algorithms are not well suitable in this project. I deduce some new algorithms during my research since the original ICP Algorithm cannot work well. Both Update Equation and Objective Function are modified. In this thesis, PCL( Point Cloud Library ) is mainly used to implement the basic function, such as nding the nearest points and sampling point cloud, and Eigen library to write the core functions( e.g. Modified Iterative Closest Point Alg ). I also use libLAS library to implement the IO operations and MeshLab to visualize the point cloud after modification.
M.S. in Computer Science, May 2015
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- Title
- SPEECH INTELLIGIBILITY AND ACCENTS IN SPEECH-MEDIATED INTERFACES: RESULTS AND RECOMMENDATIONS
- Creator
- Lawrence, Halcyon M.
- Date
- 2013, 2013-07
- Description
-
There continues to be significant growth in the development and use of speech–mediated devices and technology products; however, there is no...
Show moreThere continues to be significant growth in the development and use of speech–mediated devices and technology products; however, there is no evidence that non-native English speech is used in these devices, despite the fact that English is now spoken by more non-native speakers than native speakers, worldwide. This relative absence of non-native English speech in devices may be due in part to the costs associated with localizing speech devices, but it may also be attributable to the fact that not enough is known about user performance with accented speech in speech–mediated environments. In the absence of targeted research, developers may be relying on existing studies which focus on perception (impression) of accented speech, as a basis of decision-making. However, perception paints only part of the picture when it comes to understanding how and why people perform in certain ways and in certain environments. Three studies were conducted to answer the following questions: (1) What are the acoustic-phonetic characteristics of negatively- and positively-perceived accented speech? And how are these characteristics related to markers of intelligible speech? (2) How do participants perform on different types of accented-speech tasks? (3) What is the relationship between user perception of accented speech and user performance in response to accented speech? and; (4) How do participants perform on accented speech tasks of varying complexity? Arising out of this research, there are six recommendations for the use of accented speech in speech-mediated devices. Also, the findings of this study raise questions about inherent linguistic stereotypes which impact both our perceptions and our choices about xvi the accents we want to hear on our speech devices. A discussion about if and how these stereotypes can be altered and measured are included. Future research should examine the role of experienced non-native talkers in speech devices. Results of study one demonstrated that some experienced non-native talkers were positively-perceived by raters and may be good candidates for talkers in speech devices. A study like this would explicitly establish if listeners consistently make native vs. non-native distinctions in their preferences or if a prestige continuum emerges.
PH.D in Technical Communication, July 2013
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- Title
- Learning the Lingo? Gender, Prestige and Linguistic Adaptation in Review Communities
- Creator
- Hemphill, Libby, Otterbacher, Jahna
- Date
- 2011-11-19, 2012-02
- Publisher
- ACM Press
- Description
-
Women and men communicate differently in both face-to- face and computer-mediated environments. We study linguistic patterns considered...
Show moreWomen and men communicate differently in both face-to- face and computer-mediated environments. We study linguistic patterns considered gendered in reviews contributed to the Internet Movie Database. IMDb has been described as a male-majority community, in which females contribute fewer reviews and enjoy less prestige than males. Analyzing reviews posted by prolific males and females, we hypothesize that females adjust their communication styles to be in sync with their male counterparts. We find evidence that while certain characteristics of “female language” persevere over time (e.g., frequent use of pronouns) others (e.g., hedging) decrease with time. Surprisingly, we also find that males often increase their use of “female” features. Our results indicate, that even when they resemble men’s reviews linguistically, women’s reviews still enjoy less prestige and smaller audiences.
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- Title
- INVESTIGATION OF NOROVIRUS CROSS CONTAMINATION DURING FOOD SERVICE PROCEDURES USED IN PREPARATION OF FRESH PRODUCE
- Creator
- Suriyanarayanan, Annamalai
- Date
- 2011-11, 2011-12
- Description
-
Human norovirus (HuNoV) is considered an important cause of foodborne outbreaks, often attributed to the preparation of fresh produce by...
Show moreHuman norovirus (HuNoV) is considered an important cause of foodborne outbreaks, often attributed to the preparation of fresh produce by infected food handlers. In this investigation, methods for recovery of murine norovirus (MNV-1), a surrogate for HuNoV, from food preparatory surfaces were optimized, and MNV-1 crosscontamination between various surfaces common in a food service setting were studied. Fifty microliters of MNV-1 was inoculated onto demarcated 1 x 1 inch squares of polypropylene cutting board, stainless steel knife and spigots. After drying, MNV-1 was recovered from each surface using either a cotton swab, composite tissue or sterile sponge in combination with different eluents such as tissue culture growth medium, 3% beef extract, glycine buffer (50mM glycine, 1% beef extract), stripping solution (0.04% K2HPO4, 1.01% Na2HPO4, 0.1% Triton X-100), and Earle’s Balanced Salt Solution (EBSS). The eluent/recovery tool combinations that recovered the highest percentage of MNV-1 from cutting board were stripping solution/sponge (20%) and growth medium/swab (20%). The greatest recovery from the knife blade was achieved with the growth medium/composite tissue combination (43%), while recovery from spigots was greatest using the stripping solution/sponge (28%) and the growth medium/sponge combinations (27%). In the second phase of this investigation, human volunteers were asked to perform various tasks in order to quantify the amount of MNV-1 cross contamination between various surfaces, including bare hands, fresh-cut lettuce, and spigots. The percentage of MNV-1 transfer from hands to spigots varied from 0.06% to 3.59%, spigots to hands varied from 10% to 90.4% and lettuce to hands varied from 0.30% to 4.33%. x The results of this investigation can be used in developing a model describing the transfer pattern of HuNoV between surfaces common in retail food service, and used in developing educational materials for food service workers.
M.S. in Science, Food Safety, and Technology, December 2011
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- Title
- PULSED LIGHT INACTIVATION OF MURINE NOROVIRUS ON VARIOUS FOOD CONTACT SURFACES
- Creator
- Zhou, Zijin
- Date
- 2015, 2015-07
- Description
-
Norovirus is one of the leading causes for foodborne illness. Transmission of virus from surface to food has been known to cause a number of...
Show moreNorovirus is one of the leading causes for foodborne illness. Transmission of virus from surface to food has been known to cause a number of outbreaks. Studies of norovirus have been conducted using Murine Norovirus to simulate the behaviors. Pulsed light (PL) is a promising surface decontamination technology, which has the potential to be applied in a food service setting. PL uses intense pulses of short duration and a broad spectrum to accomplish microbial inactivation. This study evaluates the effect of PL on MNV-1, artificially inoculated onto various food contact surfaces including 304 stainless steel, glazed tile, polypropylene, and ultra-high molecular weight (UHMW) polyethylene. The virus was allowed to inoculate on the coupons for 20mins and treated with PL in a Xenon Steripulse XL-3000TM pulsed light treatment system for up to 60 s, at a distance of 8.3 cm 10.8 cm or 13.3cm from the central axis of the lamp. An infrared (IR) camera was used to record surface temperatures, in 1-s increments. After PL treatments, remaining viruses were recovered from surfaces and quantified by plaque assay. At a distance of 10.8cm, MNV-1 was reduced by 2.22-, 2.27- 2.75- and 3.12-log, after 20s treatment on inoculated stainless steel, glazed tile, UHMW polyethylene and polypropylene, respectively. After 50s treatment, MNV-1 was reduced by 4.86- and 5.93- log on glazed tile and stainless steel surface respectively. The surface temperature on tile and stainless steel increased at the rate of 1.08±0.20 and 1.28±0.32°C /s respectively. A relatively short treatment using pulsed light is sufficient to inactivate MNV-1 on the surface of materials commonly used in food preparation. The results suggest that the technology has the potential to reduce surface viral contamination in a food preparation setting.
M.S. in Food Safety and Technology, July 2015
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- Title
- WIRELESS COMMUNICATION FOR AN ACTUATED GLOVE FOR HAND REHABILITATION
- Creator
- Yuan, Ning
- Date
- 2016, 2016-12
- Description
-
Stroke survivors often experience long-term upper extremity impairment. This can greatly impair activities of daily living. The eXtension...
Show moreStroke survivors often experience long-term upper extremity impairment. This can greatly impair activities of daily living. The eXtension Glove (X-Glove) is a soft robotic device to aid hand therapy. It uses cables serving as external extensor tendons to assist digit extension and control digit flexion. Load cells are located on each motor to detect the force value of fingers. This paper provides a way to add a biofeedback function on the X-Glove and update the microprocessor to a PIC32MX795. So the X-Glove can establish a wireless communication transmit data with terminals, like PC. In order to display the biofeedback, a graphic user-interface is also developed so that therapists can optimize the therapy for each individual patient in real time.
M.S. in Biomedical Engineering, December 2016
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- Title
- Agent-based control of spatially distributed chemical reactor networks
- Creator
- Tatara, Eric, North, Michael, Hood, Cindy, Teymour, Fouad, Cinar, Ali
- Date
- 2005
- Publisher
- SPRINGER-VERLAG BERLIN
- Description
-
Large-scale spatially distributed systems provide a unique and difficult control challenge because of their nonlinearity, spatialdistribution...
Show moreLarge-scale spatially distributed systems provide a unique and difficult control challenge because of their nonlinearity, spatialdistribution and generally high order. The control structure for these systems tend to be both discrete and distributed as well and contain discrete and continuous elements. A layered control structure interfaced with complex arrays of sensors and actuators provides a flexible supervision and control system that can deal with local and global challenges. An adaptive agent-based control structure is presented whereby local control objectives may be changed in order to achieve the global control objective. Information is shared through a global knowledge environment that promotes the distribution of ideas through reinforcement. The performance of the agent-based control approach is illustrated in a case study where the interaction front between two competing autocatalytic species is moved from one spatial configuration to another. The multi-agent control system is able to effectively explore the parameter space of the network and intelligently manipulate the network flow rates such that the desired spatial distribution of species is achieved.
Endnote format citation
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- Title
- STUDY OF SALMONELLA SURVIVAL ON THE SURFACE OF FRUITS
- Creator
- Sui, Ke
- Date
- 2017, 2017-05
- Description
-
Salmonella enterica has been frequently linked to the major foodborne disease outbreaks. The molecular mechanisms underlying this pathogen...
Show moreSalmonella enterica has been frequently linked to the major foodborne disease outbreaks. The molecular mechanisms underlying this pathogen survival on the fresh fruit surface remain largely unexplored. In this study, the environmental factors that affect the survival of Salmonella strains on the surface of selected fruits were studied. Grape tomatoes (or cantaloupe peels) were inoculated in three separate trials with 1 mL Salmonella enterica serotypes Enteritidis or Typhimurium (approximately 1010-11 CFU/mL). Storage of grape tomatoes at 4 ℃ resulted in significant decrease in populations of S. Enteritidis; this trend was observed at both of the tested relative humidity with the D-value as 7.25±1.05 d and 7.28±2.34 d, respectively. At different temperatures, relative humidity only had marginal effects on the bacterial survival on the surface of grape tomato and cantaloupe. In addition, S. Typhimurium apparently survived better than S. Enteritidis on the surface of grape tomato. Furthermore, a transposonmutagenesis library with random transposon insertion mutants of S. Enteritidis and high-throughput sequencing analysis showed that the expression of genes rcsB and nlpD were hypothesized to be associated with the survival of S. Enteritidis on grape tomatoes. Inframe deletions of the two genes in S. Enteritidis were constructed by lambda red recombinase system and respective complementation mutants were also obtained for identification of the contribution of the two genes to the bacterial survival on the surface of grape tomato. Thus, this study provided microbiological and molecular microbiological basis to probe the roles of putative genes in Salmonella enterica survival on the surface of fresh fruits.
M.S. in Food Safety and Technology, May 2017
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- Title
- Devices that Assist Blind & Visually-Impaired Individuals in Swimming and Other Exercise Activities (sequence unknown), IPRO 310 - Deliverables: IPRO 310 Poster F09
- Creator
- Dykeman, Kimberly, Healton, Michaela, Lipman, Timothy, Ludwig, Ross, Park, Jay, Reilly, Jeffrey, Sarkar, Smita, Sirk, Phillip, Toro, Branden
- Date
- 2009-12
- Description
-
The objective is to develop, test, and implement assistive technology with the community that promotes safety and improves independence of...
Show moreThe objective is to develop, test, and implement assistive technology with the community that promotes safety and improves independence of blind and visually impaired (BVI) swimmers.
Sponsorship: Chicago Lighthouse for the Blind & Visually-Impaired. Notre Dame University
Deliverables for IPRO 310:Devices that Assist Blind & Visually-Impaired Individuals in Swimming and Other Exercise Activities for the fall 2009 semester.
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- Title
- Teacher Knowledge Share (Semester Unknown) IPRO 320: Teacher Knowledge Share IPRO 320 Final Presentation F08
- Creator
- Bern, David, Bochantin, Marike, Hartline, Julian, Mccall, Ian, Mick, Emily, Peake, Andrew, Pierce, Alison, Pindrik, Dmitriy, Quinn, Michael, Stanford, Carly, Tilatti, Michael
- Date
- 2008, 2008-12
- Description
-
The goal of IPRO 320 is to create an online professional network for school teachers at the kindergarten through twelfth grade level to allow...
Show moreThe goal of IPRO 320 is to create an online professional network for school teachers at the kindergarten through twelfth grade level to allow for the sharing of ideas, lesson plans and other professionally relevant information. This is a continuing project which saw limited success in the previous semester in that a functioning web site was constructed based on surveys of public school teachers but it saw very little use. This semester, the planned approach is to build on the research generated last semester by performing additional market research with teachers. Following that, IPRO 320 will rework much of the previous web site by refining some of the features, adding additional ones as the research indicates and improving the website in general, both functionally and aesthetically.
Deliverables
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- Title
- LENGTH DEPENDENT ACTIVATION IN MANDUCA SEXTA FLIGHT MUSCLE
- Creator
- Kumar, Mohit
- Date
- 2011-08-09, 2011-07
- Description
-
The flight muscle of Manduca sexta has some interesting properties. It is synchronous, like mammalian skeletal and cardiac striated muscle,...
Show moreThe flight muscle of Manduca sexta has some interesting properties. It is synchronous, like mammalian skeletal and cardiac striated muscle, but it is structurally similar to the more widely studied asynchronous insect flight muscles of Drosophila and Lethocerus. One of the main goals of the thesis is to generate a useful skinned preparation for mechanical studies in vitro. A number of different skinning protocols were tried and evaluated for preservation of structural integrity by using light and X-ray diffraction. In all muscles studied to date, isometric force is a function of the [Ca2+] of the bathing solution and also a function of the sarcomere length whereby more force is generated at a longer sarcomere length than at a shorter for the same [Ca2+]. This phenomenon is termed “length dependent activation” (LDA). To date no real studies on the force-pCa relationship has been done on Manduca sexta flight muscle. This force-pCa analysis would give us some insight into the length dependent activation (LDA) in this novel insect flight muscle system. The present studies were undertaken to characterize and analyze the force-pCa relationships in Manduca sexta. Conditions were found that allowed skinning muscles while maintaining good structural order. We found that both DLMs dorsal and ventral show length-dependent activation at longer SL. Our study also shows that ventral muscles are more cooperative than the dorsal muscles which may be related to their different functions in vivo.
M.S. in Biological, Chemical, and Physical Sciences, July 2011
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- Title
- A diary study of the disruption experiences of crew members on a jobsite (Working Paper)
- Creator
- Menches, Cindy L., Chen, Juan
- Date
- 2012-06-01, 2012-06-01
- Description
-
This article demonstrates the use of a diary technique in a construction setting. The advantage of a diary study over other methods for...
Show moreThis article demonstrates the use of a diary technique in a construction setting. The advantage of a diary study over other methods for capturing subjective experience is its ability to capture the thoughts, decisions, actions, and emotions of individuals in situ – while they are working in their natural setting – at multiple points in time during an individual’s workweek. Hence, the diary method is uniquely designed to answer questions about group-level trends as well as individual behavior. The specific goal of the study reported in this article was to derive an understanding of the types of fast decisions and actions that are made following a workflow disruption and how the disruption impacts the worker’s state of mind. The analyses revealed that there was a group-level positive correlation between experiencing a disruption and initiating a fast improvised decision and action. However, some workers tended to improvise frequently when disrupted while others did not. The analyses also revealed that the workers’ emotions fluctuated throughout the week, and two emotions – determined and interested – tended to decline following a disruption. But, some of the workers also experienced increases in their negative emotions while others did not, suggesting that not all workers react in the same way to disruptions. Consequently, the diary technique provides a novel way to identify the impact of disruptive events on worker decision-making and performance.
Sponsorship: National Science Foundation
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- Title
- MEAN EXIT TIME FOR RADIALLY SYMMETRICAL DYNAMICAL SYSTEMS DRIVEN
- Creator
- Luan, Yuanchao
- Date
- 2013, 2013-12
- Description
-
Stochastic differential equations (SDEs) driven by non-Gaussian L´evy noises have attracted much attention recently [1, 29]. In [12], the...
Show moreStochastic differential equations (SDEs) driven by non-Gaussian L´evy noises have attracted much attention recently [1, 29]. In [12], the authors studied a scalar SDE driven by a non-Gaussian L´evy motion, and numerically investigate mean exit time and escape probability for arbitrary noise intensity in one dimensional case. In the present thesis, we utilize a different strategy to explore a numerical method for the problem in two dimensional cases. To be specific, we assume the solution u(x) is radially symmetric with respect to the origin, and then represent the equation using radial coordinate, reducing the problem into one dimensional case. Then main difficulty is that, in the integral term, appears a Gauss Hypergeometric function and the unknown function u(r), which makes the error estimates complicated. We exploit some properties of Gauss Hypergeometric function, and finally make out a way for estimating the error [19]. Up to now we are only able to deal with this problem with 0 < α ≤ 1, since our numerical scheme does not converge when 1 < α < 2. Then we compare our numerical solutions with the analytical ones which are given in [3], and they coincide very well. KeyWords: Stochastic dynamical systems; non-Gaussian L´evy motion; L´evy jump measure; First exit time
M.S. in Applied Mathematics, December 2013
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- Title
- Asian American Chicago Network: A Case Study of Facebook Group Use By Immigrant Groups
- Creator
- Rao, Xi, Hemphill, Libby
- Date
- 2016, 2016
- Publisher
- ACM
- Description
-
Through analyzing data from posts and about users, we describe how one particular Facebook group helps immigrants to the U.S. use social media...
Show moreThrough analyzing data from posts and about users, we describe how one particular Facebook group helps immigrants to the U.S. use social media to build a local community. As a preliminary study in intercultural communication through social media, we analyze one case, the Asian American Chicago Network (AACN) Facebook group, and uncover common topics users discuss and relationships between user tenure and various indicators of leadership and interaction. Our small finalized results from this preliminary project suggest that members of AACN likely use it (1) to build a professional network in the U.S.A., and (2) to reinforce and affirm their Asian culture and identities.
Sponsorship: National Science Foundation Award Number 1525662
Rao, X., & Hemphill, L. (2016). Asian American Chicago Network: A Case Study of Facebook Group Use By Immigrant Groups. In Proceedings of the 19th ACM Conference on Computer Supported Cooperative Work and Social Computing Companion (pp. 381–384). New York, NY, USA: ACM. http://doi.org/10.1145/2818052.2869077
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- Title
- Variable speed motor control
- Creator
- Fry, August, Baker, Charles
- Date
- 2009, 1906
- Publisher
- Armour Institute of Technology
- Description
-
http://www.archive.org/details/variablespeedmot00frya
Thesis (B.S.)--Armour Institute of Technology
- Title
- CONSTRUCTION OF ZEBRAFISH OLFACTORY RECEPTORS INTO A MAMMALIAN EXPRESSION VECTOR FOR FUTURE LIGAND-BINDING STUDIES
- Creator
- Tian, Chen
- Date
- 2011-07, 2011-07
- Description
-
The olfactory system is an important chemosensory system. Our identification of odorants is occurred through binding of odorant molecules with...
Show moreThe olfactory system is an important chemosensory system. Our identification of odorants is occurred through binding of odorant molecules with olfactory receptors in the olfactory epithelium. In zebrafish, there are four classes of olfactory receptors: trace amine-associated receptor (TAAR), odorant receptor (OR), vomeronasal type 1-like receptor (V1R-like) and vomeronasal type 2-like receptor (V2R-like). In this study, I amplified four olfactory receptors taar1a, or1016, ora5 and olfcc1, which are respectively belong to the four olfactory receptor classes, from cDNA of the zebrafish olfactory epithelium and cloned genes into a mammalian expression vector pCI. The gene sequences of these receptors were not identical as the sequences obtained from GenBank database. The possible reasons for the inconsistency of gene sequence are discussed. I further tranfected these four cloned olfactory receptors in a mammalian cell line Hana3A. Expression of all four olfactory receptors in Hana3A cells was confirmed by immunocytochemistry. In summary, these four olfactory receptors taar1a, or1016, ora5 and olfcc1 were successfully cloned into the pCI vector and can be expressed on cell membrane surface of Hana3A cells.
M.S. in Biology, July 2011
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- Title
- SPECTRUM OBSERVATORY BASED TRAFFIC MODELING AND CHANNEL SELECTION IN SUPPORT OF DYNAMIC SPECTRUM ACCESS
- Creator
- Bacchus, Brent Roger
- Date
- 2015, 2015-05
- Description
-
It is well known that the exponential growth in popularity of wireless devices has created a demand for radio spectrum that cannot be met with...
Show moreIt is well known that the exponential growth in popularity of wireless devices has created a demand for radio spectrum that cannot be met with current regulatory policies. Despite the difficulty in procuring access to new spectrum resources, many empirical studies have indicated that the majority of spectrum is in-fact unused in the temporal, spatial and/or spectral domains, representing an untapped wealth that must be exploited. Dynamic Spectrum Access (DSA) is a promising technology which aims to improve the efficiency of future radios and alleviate the issue of spectrum under-utilization. This dissertation utilizes the data from the IIT Spectrum Observatory to develop models of channel activity on the Land Mobile Radio (LMR) band (used for critical communication by organizations such as public safety) and shows how such models can be applied to improve the performance of DSA. We demonstrate that LMR traffic may possess multi-timescale behavior – such as clustering and dispersion over different time periods – and propose a novel statistical model to account for these observations based on a multiple emission hidden Markov model. We then used this model to design a collision constrained channel selection algorithm that can permit the re-use of licensed spectrum while minimizing interference with incumbent users. The findings in this work are primarily developed for public safety, however the techniques developed are general enough to be applied to other types of traffic possessing similar characteristics. The proposed model, in particular, is well suited for further analytic work and simulations studies in this area.
Ph.D. in Electrical and Computer Engineering, May 2015
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