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Pages
- Title
- Controlling biofilms using synthetic biology approaches
- Creator
- Fang, Kuili, Park, Oh-Jin, Hong, Seok Hoon
- Date
- 2020
- Description
-
Bacterial biofilms are formed by the complex but ordered regulation of intra- or inter-cellular communication, environmentally responsive gene...
Show moreBacterial biofilms are formed by the complex but ordered regulation of intra- or inter-cellular communication, environmentally responsive gene expression, and secretion of extracellular polymeric substances. Given the robust nature of bio?lms due to the non-growing nature of bio?lm bacteria and the physical barrier provided by the extracellular matrix, eradicating bio?lms is a very di?cult task to accomplish with conventional antibiotic or disinfectant treatments. Synthetic biology holds substantial promise for controlling bio?lms by improving and expanding existing biological tools, introducing novel functions to the system, and re-conceptualizing gene regulation. This review summarizes synthetic biology approaches used to eradicate bio?lms via protein engineering of bio?lm-related enzymes, utilization of synthetic genetic circuits, and the development of functional living agents. Synthetic biology also enables bene?cial applications of bio?lms through the production of biomaterials and patterning bio?lms with speci?c temporal and spatial structures. Advances in synthetic biology will add novel bio?lm functionalities for future therapeutic, biomanufacturing, and environmental applications.
Sponsorship: NIH-R15AI130988, NSF CBET-1917130
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- Title
- Engineering Escherichia coli to produce and secrete colicins for rapid and selective biofilm cell killing
- Creator
- Jin, Xing, An, Sungjun, Kightlinger, Weston, Zhou, Jiacheng, Hong, Seok Hoon
- Date
- 2021
- Description
-
Bacterial biofilms are associated with chronic infectious diseases and are highly resistant to conventional antibiotics. Antimicrobial...
Show moreBacterial biofilms are associated with chronic infectious diseases and are highly resistant to conventional antibiotics. Antimicrobial bacteriocins are alternatives to conventional antibiotics and are characterized by unique cell-killing mechanisms, including pore formation on cell membranes, nuclease activity, and cell wall synthesis inhibition. Here, we used cell-free protein synthesis to rapidly evaluate the antibiofilm activities of colicins E1, E2, and E3. We found that E2 (with DNase activity) most effectively killed target biofilm cells (i.e., the K361 strain) while leaving nontargeted biofilms intact. We then engineered probiotic Escherichia coli microorganisms with genetic circuits to controllably synthesize and secrete colicin E2, which successfully inhibited biofilms and killed preformed indicator biofilms. Our findings suggest that colicins rapidly and selectively kill target biofilm cells in multispecies biofilms and demonstrate the potential of using microorganisms engineered to produce antimicrobial colicin proteins as live therapeutic strategies to treat biofilm-associated infections.
Sponsorship: NIH-R15AI130988
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- Title
- Editorial, "Cell-Free Synthetic Biology": Synthetic Biology Meets Cell-Free Protein Synthesis
- Creator
- Hong, Seok Hoon
- Date
- 2019
- Publisher
- MDPI
- Description
-
Sponsorship: NIH R15AI130988
- Title
- Incorporation of non-standard amino acids into proteins: challenges, recent achievements, and emerging applications
- Creator
- Jin, Xing, Park Oh-Jin, Hong, Seok Hoon
- Date
- 2019
- Description
-
The natural genetic code only allows for 20 standard amino acids in protein translation, but genetic code reprogramming enables the...
Show moreThe natural genetic code only allows for 20 standard amino acids in protein translation, but genetic code reprogramming enables the incorporation of non-standard amino acids (NSAAs). Proteins containing NSAAs provide enhanced or novel properties and open diverse applications. With increased attention to the recent advancements in synthetic biology, various improved and novel methods have been developed to incorporate single and multiple distinct NSAAs into proteins. However, various challenges remain in regard to NSAA incorporation, such as low yield and misincorporation. In this review, we summarize the recent efforts to improve NSAA incorporation by utilizing orthogonal translational system optimization, cell-free protein synthesis, genomically recoded organisms, artificial codon boxes, quadruplet codons, and orthogonal ribosomes, before closing with a discussion of the emerging applications of NSAA incorporation.
Sponsorship: NIH R15AI130988
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- Title
- Undecanoic Acid, Lauric Acid, and N-Tridecanoic Acid Inhibit Escherichia coli Persistence and Biofilm Formation
- Creator
- Jin, Xing, Zhou, Jiacheng, Richey, Gabriella, Wang, Mengya, Hong, Sung Min Choi, Hong, Seok Hoon
- Date
- 2021
- Description
-
Persister cell formation and biofilms of pathogens are extensively involved in the development of chronic infectious diseases. Eradicating...
Show morePersister cell formation and biofilms of pathogens are extensively involved in the development of chronic infectious diseases. Eradicating persister cells is challenging, owing to their tolerance to conventional antibiotics, which cannot kill cells in a metabolically dormant state. A high frequency of persisters in biofilms makes inactivating biofilm cells more difficult, because the biofilm matrix inhibits antibiotic penetration. Fatty acids may be promising candidates as antipersister or antibiofilm agents, because some fatty acids exhibit antimicrobial effects. We previously reported that fatty acid ethyl esters effectively inhibit Escherichia coli persister formation by regulating an antitoxin. In this study, we screened a fatty acid library consisting of 65 different fatty acid molecules for altered persister formation. We found that undecanoic acid, lauric acid, and N-tridecanoic acid inhibited E. coli BW25113 persister cell formation by 25-, 58-, and 44-fold, respectively. Similarly, these fatty acids repressed persisters of enterohemorrhagic E. coli EDL933. These fatty acids were all medium-chain saturated forms. Furthermore, the fatty acids repressed Enterohemorrhagic E. coli (EHEC) biofilm formation (for example, by 8-fold for lauric acid) without having antimicrobial activity. This study demonstrates that medium-chain saturated fatty acids can serve as antipersister and antibiofilm agents that may be applied to treat bacterial infections.
Sponsorship: NIH-R15AI130988; NSF REU-1757989
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- Title
- Priming Movie Product Placements: New Insights from a Cross-National Case Study
- Creator
- Balasubramanian,Siva K, Gistri, Giacomo
- Date
- 44354, 44327
- Publisher
- Taylor & Francis
- Description
-
The paucity of research on priming product placements and insights from practitioners (Study 1) motivated our investigation into how and when...
Show moreThe paucity of research on priming product placements and insights from practitioners (Study 1) motivated our investigation into how and when priming works in movie placements. Study 2 explores the impact of media priming (a media story announcing a movie placement before the movie’s release) and ad priming (a similar ad announcement) on recall through contrasts with no priming (control). US students watched a movie in a theatre after such priming for a subtle or a prominent placement. When compared to no priming, both media priming and ad priming enhanced recall for the subtle placement; no difference in recall performance emerged between no priming and either type of priming for the prominent placement. Contrast tests comparing media priming and ad priming indicated no differences in recall for either subtle or prominent placement. Study 3 replicated these recall findings with Italian moviegoers, and supported additional hypotheses and propositions for brand attitude. For the subtle (prominent) placement, attitude did not change (decreased) when comparing either media priming or ad priming with no priming. Contrast tests comparing media priming and ad priming indicated no differences in attitude outcomes, for either subtle or prominent placement. Using no priming as a baseline for comparison, the converging conclusion is that any type of priming improves (does not change) recall and does not change (worsens) attitude for subtle (prominent) placements. Overall, results do not support priming for prominent placements; selective use of any type of priming for subtle placements appears appropriate to improve recall outcomes.
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- International Journal of Advertising
- Title
- Abraham Lincoln School Art Classes leaflet, 1945-1946
- Creator
- Abraham Lincoln School
- Date
- 1945-1946
- Description
-
Course catalog for evening classes in art and design at Abraham Lincoln School (30 West Washington Street, Chicago, Illinois). Class topics...
Show moreCourse catalog for evening classes in art and design at Abraham Lincoln School (30 West Washington Street, Chicago, Illinois). Class topics were layout and typography, basic design, home planning, and photography, and the classes were taught, at least in part, by students from the Institute of Design, including Mary Henry, Morton Goldsholl, and Jack Waldheim. This catalog is Mary Henry's personal copy, and her inscription on the rear cover reads "While I was a student at the Institute of Design I taught one semester at the Abraham Lincoln School - no salary involved! This is my only copy. M.H." Date of leaflet is unknown, date range indicated is inferred from Mary Henry's dates of attendance at the Institute of Design.
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- Mary Dill Henry Papers, 1913-2021
- Title
- The Landings in North Africa November 1942
- Creator
- Moran, Charles
- Date
- 1993, 1993
- Publisher
- Naval Historical Center, Dept. of the Navy,
- Title
- Student protest at Illinois Institute of Technology, 1970
- Creator
- Illinois Institute of Technology
- Date
- 1970
- Description
-
Photograph of Illinois Institute of Technology faculty and students attending an open meeting with President John T. Rettaliata regarding...
Show morePhotograph of Illinois Institute of Technology faculty and students attending an open meeting with President John T. Rettaliata regarding student protests that occurred on IIT's campus following the fatal shooting of four students at Kent State University by the Ohio National Guard on May 4, 1970.
Show less - Collection
- John T. Rettaliata student protest photographs, 1970
- Title
- Student protest at Illinois Institute of Technology, 1970
- Creator
- Illinois Institute of Technology
- Date
- 1970
- Description
-
Photograph of student protests outside of Perlstein Hall on Illinois Institute of Technology's campus following the fatal shooting of four...
Show morePhotograph of student protests outside of Perlstein Hall on Illinois Institute of Technology's campus following the fatal shooting of four students at Kent State University by the Ohio National Guard on May 4, 1970.
Show less - Collection
- John T. Rettaliata student protest photographs, 1970
- Title
- Student protest at Illinois Institute of Technology, 1970
- Creator
- Illinois Institute of Technology
- Date
- 1970
- Description
-
Photograph of student protests outside of Perlstein Hall on Illinois Institute of Technology's campus following the fatal shooting of four...
Show morePhotograph of student protests outside of Perlstein Hall on Illinois Institute of Technology's campus following the fatal shooting of four students at Kent State University by the Ohio National Guard on May 4, 1970.
Show less - Collection
- John T. Rettaliata student protest photographs, 1970
- Title
- Student protest at Illinois Institute of Technology, 1970
- Creator
- Illinois Institute of Technology
- Date
- 1970
- Description
-
Photograph of student protesters gathered outside of President John T. Rettaliata's office in Perlstein Hall on Illinois Institute of...
Show morePhotograph of student protesters gathered outside of President John T. Rettaliata's office in Perlstein Hall on Illinois Institute of Technology's campus. Student protests took place following the fatal shooting of four students at Kent State University by the Ohio National Guard on May 4, 1970.
Show less - Collection
- John T. Rettaliata student protest photographs, 1970
- Title
- Student protest at Illinois Institute of Technology, 1970
- Creator
- Illinois Institute of Technology
- Date
- 1970
- Description
-
Photograph of student protesters gathered outside of President John T. Rettaliata's office in Perlstein Hall on Illinois Institute of...
Show morePhotograph of student protesters gathered outside of President John T. Rettaliata's office in Perlstein Hall on Illinois Institute of Technology's campus. Student protests took place following the fatal shooting of four students at Kent State University by the Ohio National Guard on May 4, 1970.
Show less - Collection
- John T. Rettaliata student protest photographs, 1970
- Title
- Benny Goodman receiving the IIT Hall of Fame medal and plaque from IIT Provost Sidney Guralnick, Chicago, Illinois, ca. 1980
- Date
- 1980
- Description
-
Photograph of Provost Sidney Guralnick presenting the IIT Hall of Fame medal and plaque to Benny Goodman. Goodman attended Lewis Institute in...
Show morePhotograph of Provost Sidney Guralnick presenting the IIT Hall of Fame medal and plaque to Benny Goodman. Goodman attended Lewis Institute in 1923 as a high school sophomore while also playing the clarinet in a dance hall band. The legendary orchestra conductor and jazz musician received an honorary LL.D. degree from IIT in 1968. Photographer unknown. Date listed is approximate.
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- Office of Communications and Marketing photographs, 1905-1999
- Title
- Student protest at Illinois Institute of Technology, 1970
- Creator
- Illinois Institute of Technology
- Date
- 1970
- Description
-
Photograph of student protests outside of Perlstein Hall on Illinois Institute of Technology's campus following the fatal shooting of four...
Show morePhotograph of student protests outside of Perlstein Hall on Illinois Institute of Technology's campus following the fatal shooting of four students at Kent State University by the Ohio National Guard on May 4, 1970.
Show less - Collection
- John T. Rettaliata student protest photographs, 1970
- Title
- Student protest at Illinois Institute of Technology, 1970
- Creator
- Illinois Institute of Technology
- Date
- 1970
- Description
-
Photograph of Illinois Institute of Technology faculty and students attending an open meeting with IIT President John Rettaliata regarding...
Show morePhotograph of Illinois Institute of Technology faculty and students attending an open meeting with IIT President John Rettaliata regarding student protests that occurred on IIT's campus following the fatal shooting of four students at Kent State University by the Ohio National Guard on May 4, 1970.
Show less - Collection
- John T. Rettaliata student protest photographs, 1970
- Title
- Student protest at Illinois Institute of Technology, 1970
- Creator
- Illinois Institute of Technology
- Date
- 1970
- Description
-
Photograph of Illinois Institute of Technology faculty and students attending an open meeting with IIT President John Rettaliata regarding...
Show morePhotograph of Illinois Institute of Technology faculty and students attending an open meeting with IIT President John Rettaliata regarding student protests that occurred on IIT's campus following the fatal shooting of four students at Kent State University by the Ohio National Guard on May 4, 1970.
Show less - Collection
- John T. Rettaliata student protest photographs, 1970
- Title
- School of Architecture and Planning Faculty Meeting, April 10 and 16, 1970
- Creator
- Illinois Institute of Technology, Illinois Institute of Technology. College of Architecture
- Date
- 1970-04
- Description
-
Digitized 7" open reel-to-reel tape recording of the Illinois Institute of Technology's School of Architecture and Planning faculty meeting,...
Show moreDigitized 7" open reel-to-reel tape recording of the Illinois Institute of Technology's School of Architecture and Planning faculty meeting, 1970. The meeting was opened with an unidentified person (possibly George Danforth, chairman of Architecture or Dr. Antonis Tritsis) reading the statement by Ludwig Mies van der Rohe (as director of the Armour Institute of Technology department of architecture) published in the 1939-1940 AIT Bulletin (academic catalog) identifying the philosophy and content of the 5-year architecture course. The IIT architecture curriculum is discussed before meeting begins.
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- Ludwig Mies van der Rohe Memorial collection, 1969-1970
- Title
- Henry Heald, 1937
- Date
- 1937
- Description
-
Portrait of Dean Henry T. Heald taken the year he was named as Acting President of Armour Institute of Technology after the resignation of Dr....
Show morePortrait of Dean Henry T. Heald taken the year he was named as Acting President of Armour Institute of Technology after the resignation of Dr. Willard E. Hotchkiss. Heald would be named President of Armour Tech shortly after, and remained President of Armour Tech and, later, Illinois Institute of Technology, until 1952. Photograpger unknown.
Show less - Collection
- Office of Communications and Marketing photographs, 1905-1999
- Title
- Student protest at Illinois Institute of Technology, 1970
- Creator
- Illinois Institute of Technology
- Date
- 1970
- Description
-
Photograph of student protesters gathered outside of President John T. Rettaliata's office in Perlstein Hall on Illinois Institute of...
Show morePhotograph of student protesters gathered outside of President John T. Rettaliata's office in Perlstein Hall on Illinois Institute of Technology's campus. Student protests took place following the fatal shooting of four students at Kent State University by the Ohio National Guard on May 4, 1970.
Show less - Collection
- John T. Rettaliata student protest photographs, 1970