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Title: AN ENERGY EFFICIENT ROUTING PROTOCOL FOR WIRELESS SENSOR NETWORKS
Creator: Lara, Aurobinda
Date: 2012-04-27, 2012-05
Description: Wireless distributed microsensor systems will enable the reliable monitoring of a variety of environments for both civil and military...
Show moreWireless distributed microsensor systems will enable the reliable monitoring of a variety of environments for both civil and military applications. A wireless sensor network consists of nodes that can communicate with each other via wireless links. One way to support efficient communication between sensors is to organize the network into several groups, called clusters, with each cluster electing one node as the head of cluster. Energy efficiency is of great importance for the wireless sensor network (WSN). A popular way to save energy is to construct clusters for data aggregation and forwarding. In this thesis a distributed cluster algorithm is studied to improve the energy consumption efficiency. It was observed that the cluster head has to lie within the transmission range of the base station (sink node) and the distance between cluster head and base station is critical for the energy consumption performance, we proposed a pseudo-cluster and virtual hierarchical clustering scheme (PC-LEACH), which considers the power level of the non-cluster head nodes and the residual energy level during the cluster head selection stage. Consequently we could better balance the chance of being cluster head for all nodes. Simulation results show that the scheme is able to result in longer network lifetime than the well-known protocol LEACH.
M.S. in Electrical Engineering, May 2012
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Title: ENHANCEMENT OF BIODESULFURIZATION IN RHODOCOCCUS SPECIES (IGTS8) BY THE EXPRESSION OF VITREOSCILLA HEMOGLOBIN
Creator: Shivdas, Vrushali D.
Date: 2013, 2013-07
Description: The bacterium Rhodococcus sp. IGTS8 contains the dsz operon, which encodes a three enzyme pathway (the “4S pathway”) that is able to...
Show moreThe bacterium Rhodococcus sp. IGTS8 contains the dsz operon, which encodes a three enzyme pathway (the “4S pathway”) that is able to mineralize the sulfur contained in dibenzothiophene (DBT), an organic sulfur containing molecule found in petroleum. The gene vgb, which encodes Vitreoscilla hemoglobin (VHb), has shown wide usefulness in enhancing productivity and other useful properties when expressed in heterologous hosts. We engineered strain IGTS8 to express VHb and measured the effects on growth and desulfurization of DBT, using minimal medium containing DBT as the sole source of sulfur. VHb was clearly detected in the engineered strain using the standard COdifference spectral analysis, but its level (0.38-0.63 nmoles/gm wet weight of cells) was about 10-fold lower than commonly seen for expression of VHb in other heterologous bacterial hosts. The VHb-expressing strain was tested for growth at both low and high aeration in minimal medium containing DBT as sole sulfur source; growth was about 50% lower at low aeration compared with high aeration. Despite this, metabolism of DBT (as detected by accumulation of the end product of the 4S pathway, 2-Hydroxy biphenyl (2-HBP), in the growth medium) was about 30 % higher in the low aeration compared to the high aeration culture. A possible explanation for these results is direct enhancement of the first two (monooxygenase) steps in conversion of DBT to 2-HBP. It was thus concluded from the studies that the expression of vgb in Rhodococcus sp. IGTS8 enhances the process of biodesulfurization under conditions of low aeration
M.S. in Biology, July 2013
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Title: Test of a new type of storage cell
Creator: Allyn, A. J., Torrance, R. S.
Date: 2009, 1906
Publisher: Armour Institute of Technology
Description: http://www.archive.org/details/testofnewtypeofs00ally
Thesis (B.S.)--Armour Institute of Technology

Title: DYNAMICS OF A THREE-DIMENSIONAL FOUR-BAR LINKAGE SUBJECT TO RANDOM EXTERNAL FORCING
Creator: Lytell, Mark R.
Date: 2011-11-15, 2011-12
Description: This thesis explores the dynamics of a three-dimensional four-bar mechanical linkage subject to random external forcing. The Lagrangian...
Show moreThis thesis explores the dynamics of a three-dimensional four-bar mechanical linkage subject to random external forcing. The Lagrangian formulation of the equations of motion are index-3 stochastic di erential-algebraic equations (SDAE) that describe the time evolution of the sample paths of the generalized coordinates, velocities, and Lagrange multipliers as stochastic processes. We solve the SDAEs using two di erent approaches: inverse dynamics, Case Study 1, via independent, successive solution of the nonlinear equations for each kinematic variable, where the time evolution of one generalized coordinate is prescribed; and direct dynamics, Case Study 2, via direct solution of the SDAEs in the index-1 formulation, using fourth-order stochastic backward di erentiation formula (BDF) with modi ed Newton iteration and position and velocity stabilization (Ascher and Petzold [2]), where the (deterministic) input driving torque is prescribed. For the particular application of a three-dimensional swing gate security system, we conduct numerical experiments for both approaches. In Case Study 1, we simulate the random external forcing as a Gaussian wind speed process that applies stochastic wind drag onto the gate. The kinematic variables are deterministic, while the required input driving torque is a stochastic process. In Case Study 2, we apply the external forcing as a resistive torque with additive Gaussian noise modeling the wind drag; the kinematic variables are stochastic processes. For both cases, we apply four mean wind speeds: 0 mph (deterministic only), 10 mph, 20 mph, and 30 mph, from which we compute the deterministic solution and three stochastic sample paths for each stochastic process. The overall conclusions are that direct solution is possible for inverse dynamics, that the solution of index-1 SDAEs in multibody dynamics is tractable since the mass matrix is symmetric and positive de nite, and that the deterministic solution is the expectation of the sample paths.
M.S. in Applied Mathematics, December 2011
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Title: GENOME ANNOTATION AND PHYLOGENETIC ANALYSIS OF 27 SALMONELLA STRAINS BASED ON BIOINFORMATIC ANALYSIS OF RESPECTIVE GENOMES AND THREE GENES
Creator: Li, Xinyue
Date: 2013-04-15, 2013-05
Description: Salmonella is the most common food-borne bacterial infectious pathogen worldwide. Different serovars of Salmonella are capable of infecting...
Show moreSalmonella is the most common food-borne bacterial infectious pathogen worldwide. Different serovars of Salmonella are capable of infecting different kinds of hosts, such as humans, mice, pigs, chickens, and can also lead to different syndromes, such as enterica fever, enterocolitis and diarrhea, bacteremia and chronic asymptomatic carriage. Although Salmonella strains are quite diverse, strains within the same serovar usually infect the same host and cause similar symptoms. Thus, it is important, especially in food-borne disease outbreaks, to know which type of Salmonella is present. The current method of typing Salmonella is based on the Kaufmann-White scheme and MLEE, which are laborious and expensive. Although the reliability of this method has not been previously verified, the evolutionary relationship reflected by phylogenetic trees can be a possible alternative to the way of typing the Salmonella strains; this method would be less labor intensive and more economical. MLST is considered as a “gold standard” of typing for many species includes Salmonella. And genome sequence, which certainly reflects the evolutionary relationship of strains, is the most ideal data to construct a more reliable phylogenetic tree; however, genome sequencing is also a laborious and expensive process. Thus, conserved and ubiquitous gene data, which can be accessed with little effort, are generally used to minimize cost. Using16s rRNA is the most widely used method. In this study, 27 Salmonella genome sequences are annotated with RAST, and phylogenetic trees are constructed using three software, (phylip3.69, MEGA5.1, and CVTree). And MLST is also used to construct phylogenetic tree in this study, and the result is used to be compared with genome phylogenetic tree to find a more reliable reference tree. Although Neighbor-Joining method is the only algorithms x available in CVTree, phylip3.69 and MEGA5.1 are capable to use three separate algorithms(Maximum Parsimony, Maximum Likelihood, and Neighbor-Joining, respectively). Finally, these trees are compared in an effort to find a good alternative to replace the reference phylogenetic tree. In this study, it was determined that the groEL gene would be the best replacement.
M.S. Biological and Chemical Sciences, May 2013
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Title: Reclaiming the Lake: Ecological Infrastructure as a Catalyst for Change
Creator: Searle, Gideon
Date: 2010-07-30

Title: SEARCHING ENCRYPTED CLOUD DATA
Creator: Urs, Karthik Mahendra Raje
Date: 2011-05-04, 2011-05
Description: The major setback of the cloud is its inability to induce strong trust in its users. For us, the users of the cloud, to be sure of the...
Show moreThe major setback of the cloud is its inability to induce strong trust in its users. For us, the users of the cloud, to be sure of the security of the data from other users, we have to trust cloud. In most of the cases, say if the data were to be hosted on Microsoft’s servers, we can trust Microsoft in its policies to prevent unauthorized access to it from other users (via hacking). But, the issue that is haunting the cloud is this - ”How can we be sure that our data is safe from Cloud service providers themselves?” The most intuitive solution for the cloud service provider to gain trust is to insist we encrypt our data. That is an irrefutable act of honesty from the cloud. But this has some obvious setbacks, few of which would negate the very advantages of cloud. One such issue that dictates the scope of this thesis is this - Typical cryptographic algorithms are not amenable to search. Now, if we were to implement a search engine on cloud (which has the data encrypted), the native search techniques have to be extended on to the cryptographic domain so that the cloud can perform the tasks of a search engine. This thesis explores the solutions to the above mentioned problem of searching over encrypted data, specifically using a secure pre-processed index approach. Though significant ideas have been proposed in the area, there is no concrete implementation to analyze if it can be used in the industrial setting. The main goal of the thesis is to implement a system which supports a variety of searching techniques. We make use of Amazon EC2 and test out the designed systems to evaluate how feasible they are for immediate consumption by the industry.
M.S. in Computer Engineering, May 2011
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Title: VALIDATION OF BAKING TO INACTIVATE SALMONELLA IN HIGH-PROTEIN AND HIGH-FAT MODEL FOODS
Creator: Wang, Wenqian
Date: 2017, 2017-07
Description: Baked food products, such as dry pet foods, undergo changes of temperature and water activity (aw) during forced hot air processes. As one of...
Show moreBaked food products, such as dry pet foods, undergo changes of temperature and water activity (aw) during forced hot air processes. As one of the most thermal resistant microorganisms in low-moisture/intermediate moisture foods, Salmonella’s thermal inactivation kinetics during these processes is not well understood and difficult to predict. The objective of this study was to evaluate thermal inactivation kinetics of Salmonella enterica serovar Agona 447967 in model high-protein (HP) and high-fat (HF) multiple-component foods baked in a laboratory-scale oven, as influenced by oven temperature and relative humidity (RH). Model high-protein and high-fat foods, formulated with wheat flour, soy protein and soy oil, were inoculated with Salmonella Agona to a level of ~9 log CFU/g, and mixed to form a homogenous dough. Dough samples (57 mm diameter x 6 mm thick) were baked (3 samples per dwell time, 6 dwell times per condition) in a lab-scale oven at 120°C (10% RH) and 85°C (20%, 35% RH, 50% RH), respectively. Temperature and aw were measured at the surface and geometric center of the product during baking. Processed samples were collected in sterile bags and immediately cooled in an ice-water bath. Salmonella was enumerated on trypticase soy agar supplemented with yeast extract and incubated at 37°C for 24 h. Similar reductions (p>0.05) of 5.12-, 5.11-, 4.55-, and 4.78-log CFU/g were achieved after 40 min at 120°C/10% RH, 90 min at 85°C/20% RH, 50 min at 85°C/35% RH, 8 min at 85°C/50% RH, respectively, in the high-protein model food. Similar results were achieved in the high-fat matrix. The aw at the geometric center of the product (initially at aw =0.98) did not change appreciably during baking, while the aw at the product surface, the location of least lethality, decreased significantly (p<0.05) during baking; the decreases were more pronounced at lower oven RH. The results indicate that thermal inactivation of Salmonella Agona was driven by temperature and relative humidity in the oven. Higher temperature and higher relative humidity level led to greater Salmonella inactivation.
M.S. in Food Process Engineering, July 2017
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Title: The synchronous motor
Creator: Sleezer, Frank W., Williams, Wallace, Kelkenney, Artemas A.
Date: 2009, 1907
Publisher: Armour Institute of Technology
Description: http://www.archive.org/details/synchronousmotor00slee
Thesis (B.S.)--Armour Institute of Technology; Bibliography: leaves 35-37

Title: Hostile Spaces and Hostel Spaces: Re-Visioning Highway Infrastructure
Creator: Smith, Kevin
Date: 2010-07-30

Title: Test and report of a modern ice plant
Creator: Heim, Karl, Fitch, Joseph E, Jones, Charles H, Hirschfeld, George
Date: 2009, 1909
Publisher: Armour Institute of Technology
Description: http://www.archive.org/details/testreportofmode00heim
Thesis (B.S.)--Armour Institute of Technology

Title: THERMAL STABILITY OF FOOD ALLERGENS AND NONALLERGENIC PROTEINS: A COMPARATIVE STUDY
Creator: Wu, Yan
Date: 2015, 2015-07
Description: Thermal stability has been proposed as a criterion to assess the allergenic potential of genetically modified foods, but there is a lack of...
Show moreThermal stability has been proposed as a criterion to assess the allergenic potential of genetically modified foods, but there is a lack of information on the relative thermal stability of food allergens vs. nonallergenic proteins. This study compared the thermal stability of several paired food allergens and nonallergenic proteins by measuring the changes in their solubility, antigenicity and thermodynamic properties after thermal treatment using BCA total protein assay, inhibition ELISA assay, Differential Scanning Calorimetry and Far-UV Circular Dichroism Spectroscopy. The selected protein pairs included bovine α-lactalbumin (allergen) vs. human α-lactalbumin (nonallergen), peanut lectin (allergen) vs. concanavalin A (nonallergen), soybean trypsin inhibitor (allergen) vs. lima bean trypsin inhibitor (nonallergen). With respect to protein solubility, a greater thermal stability was observed for: (1) bovine α-lactalbumin (bovine ALA) when it was autoclaved in water compared with human α-lactalbumin (human ALA) (2) peanut lectin when it was boiled or autoclaved in water compared with concanavalin A (ConA) and (3) soybean trypsin inhibitor (STI) when it was boiled in water or PBS, or autoclaved in water compared with lima bean trypsin inhibitor (LTI). Regarding TM values determined by DSC: (1) bovine ALA was more thermally stable than human ALA under both dry-heat treatment and moist-heat treatment (2) ConA was more thermally stable than peanut lectin during moist-heat treatment, while a greater thermal stability was observed for peanut lectin when the protein was under dry-heat treatment (3) LTI was more thermally stable than STI during heating in water or under dry-heat treatment, while a greater thermal stability was observed for STI when the protein was heated in PBS. Based on CD analyses, (1) bovine ALA was more thermally stable than human ALA (2) peanut lectin was more thermally stable than ConA (3) STI and LTI showed similar thermal stability. With respect to antigenicity, a greater thermal stability was observed for: (1) human ALA when it was autoclaved in water compared with bovine ALA (2) ConA when it was boiled or autoclaved in water, or under dry-heat treatment at 176℃ compared with peanut lectin (3) STI when it was autoclaved in PBS compared with LTI. In summary, allergens tended to be more thermally stable than nonallergenic proteins with respect to solubility, but there was no consistent trend in the relative thermal stability of these protein pairs regarding the antigenicity or the TM values. No correlation between thermal stability of the proteins and their allergenic potential was observed.ConA (3) STI and LTI showed similar thermal stability. With respect to antigenicity, a greater thermal stability was observed for: (1) human ALA when it was autoclaved in water compared with bovine ALA (2) ConA when it was boiled or autoclaved in water, or under dry-heat treatment at 176℃ compared with peanut lectin (3) STI when it was autoclaved in PBS compared with LTI. In summary, allergens tended to be more thermally stable than nonallergenic proteins with respect to solubility, but there was no consistent trend in the relative thermal stability of these protein pairs regarding the antigenicity or the TM values. No correlation between thermal stability of the proteins and their allergenic potential was observed.
M.S. in Food Safety and Technology, July 2015
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Title: DEVELOPMENT OF AN EMBEDDED CONTROLLER FOR TWO QUADRANT OPERATION OF BLDC MOTORS IN AUTOMOTIVE APPLICATIONS
Creator: Liu, Yizhe
Date: 2011-07-06, 2011-07
Description: Due to growing concerns over the availability of non-renewable petroleum products and the increasing emphasis on protecting our environment...
Show moreDue to growing concerns over the availability of non-renewable petroleum products and the increasing emphasis on protecting our environment from green house gas emissions, plug-in hybrid electric vehicle and hybrid electric vehicles have been gaining increasing popularity over recent years. This has led to the design of advanced drive systems using different electric motors such as BLDC, PMSM, IM and SRM. An electric motor/generator requires controller that not only has a strong I/O control function, but also high speed signal processing ability for realizing real time control. Therefore, a DSP (Digital Signal Processors), which integrates flexible multiple PWM signal generator, high speed high precision ADC and encoder interface for motor speed and position feedback, is considered as one of the strongest controllers for electric motor control. Owing to a growing emphasis on identification, interference, and communication of security services of drive train in intelligent PHEVs and HEVs, a DSP can also be used to identify and isolate security issues in the integrity of the drive train in PHEVs and EVs. This thesis has two main contributions: a) it focuses on the development of an embedded controller using TMS320LF2812 DSP to realize two quadrant control of the BLDC; and b) it develops an automotive test bench to identify and investigate some potential security issues using the embedded controller’s eCAN communication function.
M.S. in Electrical Engineering, May 2011
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Title: DYNAMIC SIMULATION AND LOAD MODELING FOR VOLTAGE RECOVERY STUDIES WITH THREE-PHASE AND SINGLE-PHASE INDUCTION MOTORS
Creator: Zhang, Jingpei
Date: 2017, 2017-05
Description: Transient stability topics have been popular in modern power systems and time domain simulation provides an efficient evaluation for...
Show moreTransient stability topics have been popular in modern power systems and time domain simulation provides an efficient evaluation for correlative studies. So far, we have been working on developing advanced time-domain simulator TS3ph, which implements simulation on electromechanical level but with three-phase completed network model to enable unbalanced modeling and simulation. In this paper, modeling and development work of TS3ph are being introduced. Positive-sequence equivalent and three-phase based models, including static var compensator, governor and three-phase induction motor models, are built and validated to evaluate their dynamic behaviors in transient state. Moreover, research work on single phase induction motor model development, including important motor behaviors such as motor stalling, is also introduced in this paper for voltage collapse and recovery study. This is indeed the first time to apply such model on electromechanical simulator as well.
M.S. in Electrical Engineering, May 2017
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Title: TEMPERATURE PROFILES THROUGH THE SHELL IN EGGS HEATED BY INFRARED ENERGY
Creator: Guo, Jingxin
Date: 2012-07-30, 2012-07
Description: Infrared heating is an effective method for surface pasteurization of shell eggs. As the external temperature reaches the inactivation...
Show moreInfrared heating is an effective method for surface pasteurization of shell eggs. As the external temperature reaches the inactivation temperature, internal temperatures could become great enough to denature albumen. However, the internal temperature of albumen at the shell cannot be measured directly. The purpose of this study is to use experimental data to understand the response of the albumen temperature at the shell to infrared radiation impinging at its outer surface. Fresh eggs were obtained from a local producer and refrigerated until needed. They were prepared for each experiment by overnight equilibration with ambient conditions. Each egg was placed on a metal stage over which an infrared lamp was positioned. The stage allowed a K-type thermocouple to be inserted through a small hole in the shell opposite of the shell area facing the lamp. The thermocouple was pushed into the egg such that the tip was against the inner surface of the shell just underneath the exposed area. The external temperature of this area was measured by an infrared pyrometer. Variables for these experiments were temperature of the IR lamp (277°C, 329°C, 391°C and 452°C), distance between shell egg and IR lamp (3.13 cm – 15.83 cm), and treatment time. All experiments share the same initial lag around 30s, suggested that the initial lag did not vary with changing of lamp-egg distances and lamp temperatures. After the initial lag, internal and external temperatures were found to increase at the same rate regardless of experimental parameters, indicating and equilibrium between the infrared energy impinging on the surface and its dissipation in the interior of the egg. The difference of temperature distributes between 10°C to 20°C. However, lowering lamp temperatures or increasing distances did not change this difference significantly. Moreover, the temperature gap sustains the same temperature after initial lag. The results showed that the internal temperature could be inferred via measuring external temperature. This is valuable in processing to maximize external surface temperature while protecting heat sensitive albumen. Future work will involve modeling the heating phenomenon to determine if knowing only the lamp temperature and distance is sufficient for predicting internal temperature.
M.S. in Food Processing Engineering, July 2012
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Title: Learning Company, Literacy Center, Johannesburg, South Africa
Creator: Gray, Aisling
Date: 2010-07-29

Title: SYSTEM-ON-CHIP ARCHITECTURES FOR SIGNAL PROCESSING AND COMMUNICAnONS
Creator: Niu, Sufeng
Date: 2012-04-30, 2012-05
Description: System-On-Chip (SOC) is one of the most popular Computer Aided Design (CAD) methodologies in electronic system design. In this research, SOC...
Show moreSystem-On-Chip (SOC) is one of the most popular Computer Aided Design (CAD) methodologies in electronic system design. In this research, SOC design is investigated in two different types of applications: i) low cost and power efficient applications; and ii) high performance computing applications. To explore low cost and power efficient design, a microcontroller based wireless medical system is investigated. Two wireless communication protocols for medical applications and patient monitoring are analyzed. In addition, the ZigBee stack developed by TI and a medical amplifier are discussed. For high performance SOC applications, implementations of several matrix operations are examined. An improved fixed-point hardware design of QR decomposition is introduced and optimized for Xilinx FPGAs. A Givens Rotation algorithm is implemented using a folded systolic array and the CORDIC algorithm. This approach is highly suitable for high-speed FPGAs or ASIC designs. It is found that the Xilinx XC5VLX110T FPGA is capable of running the QR decomposition at 246MHz.
M.S. in Electrical Engineering, May 2012
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Title: OPTIMAL BIDDING STRATEGY FOR HYDRO UNIT
Creator: Zhu, Renchen
Date: 2013-04-30, 2013-05
Description: The bidding price for the renewable energy is very different from some traditional energy, like gas, coal and so on, because when we produced...
Show moreThe bidding price for the renewable energy is very different from some traditional energy, like gas, coal and so on, because when we produced the renewable energy, the only cost is the generator unit cost, for example, wind power, water power and so on. People never need to pay the fuel price for the solar, water or wind. So here come one question how can we decide the energy price for these power. As we all know, the bidding price decided the profit for the generating company. Nowadays, more and more researches have been done in this field for every company want a good price to earn the highest profit. But for the renewable energy, they are different from the traditional energy which price is determined by the fuel price. So how to decide the bidding price of the renewable energy will be a good topic and question for us. To solve this problem, I will try to use an idea called minimum the imbalance in order to maximum the profit for those owners. This idea first has been applied in the wind unit. For my thesis, I will apply this idea to the hydro unit both with the storage and without storage. For each part, I will use some data to test. By these testing, the result will show this bidding strategy will be better.
M.S. in Electrical Engineering, May 2013
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Title: ISOLATION OF MONOSPECIFIC ANTIBODY AGAINST SPLICED VARIANT OF DYSTROPHIN PROTEIN USING YEAST SURFACE DISPLAY TECHNIQUE
Creator: Saraswathi, Raj Prabu Vijayakumar
Date: 2011-05-04, 2011-05
Description: Dystrophin gene is the largest in the human genome with 79 exons covering greater than 0.1% of the total genome, located on the Xp21 locus of...
Show moreDystrophin gene is the largest in the human genome with 79 exons covering greater than 0.1% of the total genome, located on the Xp21 locus of the “X” chromosome resulting in a 427kDa protein, “Dystrophin”. Dystrophin is an important cytoskeletal protein which belongs to the β-Spectrin/α-actinin family of proteins. It comprises of an amino terminal domain, alpha helical coiled structure COOH domain, central rod region with 24 STRs and four proline rich hinge regions. It plays a vital role in localizing the Dystrophin glycoprotein complex (DGC) in the Sarcollema and is associated with the DGC in controlling the signaling events of certain proteins associated with DGC. The large size of the gene makes it more vulnerable to mutations resulting in partially functional or non-functional Dystrophin. The absence of Dystrophin results in disruption of sub sarcolemma-extracellular matrix linkage, loss of nitric oxide, progressive muscle weakening and muscle wasting leading to the death of patients typically before the end of their teenage. In certain cases alternatively spliced isoforms produce Dystrophin with reduced length yet stable and completely functionality. The main focus of this project was to select monospecific antibody against the more stable alternatively spliced variant D14 (15”16”) 17, which is functional and more stable compared to unspliced parent D14:17. The yeast surface display technique was used to effectively screen and select the yeast scFv clones containing the monospecific antibody against our target spliced variant D14 (15”16”) 17 protein and D2:3. The yeast scFv sub population was enriched by repeated MACS and FACS selection. Test colonies picked from the enriched scFv pool were confirmed via PCR and restriction digestion analysis. The scFv for the respective antigens were then sub cloned into pPnnl-9 secretion vector using YVH10 yeast cells via LiTRAFCO method. It was clear that by repeated MACS and FACS selection the scFv pool can be enriched and the yeast scFv clone sub population can be reduced to a significant level. The scFv sub cloned into Pnnl-9 secretion vector can be purified using affinity chromatography and the further affinity and avidity studies can be conducted.
M.S. in Biology, May 2011
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Title: L2E ESTIMATOR FOR THE CATEGORICAL MODEL WITH ELASTIC NET PENALTY
Creator: Wang, Yuan
Date: 2017, 2017-07
Description: The logistic regression model is an important generalized linear model for the categorical data. The maximum likelihood estimation is mostly...
Show moreThe logistic regression model is an important generalized linear model for the categorical data. The maximum likelihood estimation is mostly used in estimating the parameters of the logistic regression model. However, the maximum likelihood estimation is very sensitive to outliers which will cause the inaccuracies of the fitted parameters and model selection in high-dimensional regression. Chi and Scott (2014) demonstrated by simulation that minimizing the integrated square error or L2 estimation (L2E) is a robust method to fit 2-class categorical models. They also showed that the L2E estimation method can select the right model even in the presence of many outliers in high dimensional scenarios. In my thesis, I extended the L2E estimation method from 2-class to 3-class based on the MM algorithm by Chi and Scott (2014). Then I demonstrated the properties above for 2-class categorical models are also applicable to 3-class ones.
M.S. in Applied Mathematics, July 2017
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