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Pages
- Title
- Utilizing Image Processing in Evaluation of Fibroblast Stimulation for Collagen Remodeling
- Creator
- Yoon, Shin Hae
- Date
- 2023
- Description
-
This research delves into the realm of image processing as a pivotal component in the evaluation of fibroblast stimulation for collagen...
Show moreThis research delves into the realm of image processing as a pivotal component in the evaluation of fibroblast stimulation for collagen remodeling. The study focuses on unraveling the intricate synergy between electrospun silk fibroin-carbon nanotube (SF-CNT) fibers and electrical stimulation, working in harmony to enhance tissue regeneration. Building upon our previous work, we successfully engineered SF-CNT fibers through the electrospinning process, yielding highly aligned structures reminiscent of natural extracellular matrix proteins. These fibers were fortified with water stability through post-treatment with ethanol vapor, while subtle additions of carbon nanotubes (CNTs) significantly improved fiber alignment, strength, and conductivity without compromising biocompatibility. This innovative platform served as a cell culture matrix for fibroblasts harvested from pelvic organ prolapse (POP) patients, facilitating electrical stimulation that triggered a substantial increase in collagen production. In this study, we harnessed the power of various image-processing software tools, including ImageJ and Python, to analyze immunostained images of fibroblasts obtained from POP patients. Under carefully tailored electrical stimulation conditions, the stimulated cells exhibited an astonishing up to 11.97-fold increase in alpha-smooth muscle actin (α-SMA) expression, unequivocally signifying the successful activation of myofibroblasts. Additionally, in an animal model employing LOX-knockout mice to mimic collagen disorders associated with POP, the application of optimized electrical stimulation conditions for patient 003 led to a remarkable surge in collagen production and structural enhancement, underlining the potential of electrical stimulation in expediting tissue remodeling. Intriguingly, fibroblasts from patient 005 and patient 006 exhibited a distinct response, shedding light on the influence of POP severity on cellular behavior. This study firmly reinforces the imperative of personalized therapeutic approaches, emphasizing the need to customize treatment strategies to align with individual patient characteristics through innovative biological image analysis techniques.
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- Title
- Effect of organic acid treatment in reducing Salmonella on six types of sprout seeds
- Creator
- Yang, Dachuan
- Date
- 2023
- Description
-
Fresh sprouts present a special food safety concern as their growing conditions also favor the growth of pathogens such as Salmonella....
Show moreFresh sprouts present a special food safety concern as their growing conditions also favor the growth of pathogens such as Salmonella. Contamination in sprouts often originates from the seeds used for sprouting. The Produce Safety Rule requires that seeds used to grow sprouts be treated to reduce pathogens. The treatments may be applied by sprout growers or by seed suppliers. Although 20,000 ppm calcium hypochlorite is the most used seed treatment method, the high chlorine level can be hazardous to workers and the environment. Alternative seed treatment methods that are safe and environmentally friendly are needed. In addition, a post-treatment drying step is needed when seed suppliers are using chemical seed treatment methods. This study evaluated the efficacy of an organic acid solution for reducing Salmonella on six types of seeds (alfalfa, clover, radish, mung bean, onion, and broccoli). The impact of treatment on seed germination and sprout yield was also examined. Ten grams of seeds inoculated with a five-serotype cocktail of Salmonella were pre-rinsed with 40 ml of water twice and treated with 75.7 ml of the organic acid solution for 1 hour. The treated seeds were either not rinsed or rinsed with 40 ml of water twice before being dried in the biological safety cabinet for 24 hours. The Salmonella level, germination percentage, and sprout yield of seeds treated with water, seeds treated with the organic acid solution, seeds treated with organic acid, dried, and rinsed, and seeds treated with organic acids, dried, and not rinsed were compared. Salmonella reductions that could be achieved with this organic acid solution treatment were less than 0.5 log CFU/g without drying, 0.6-2.0 log CFU/g with drying and rinse, or 1.6-2.9 log CFU/g with drying and no rinse. Drying significantly enhanced the treatment efficacy (p < 0.05 ) on alfalfa, radish, mung bean, and onion seeds. If seeds were not rinsed after treatment, the log reductions achieved on mung bean and onion seeds were significantly higher (p < 0.05). If seeds were treated and rinsed, the germination rates of six types of seeds were not affected (p > 0.05) regardless of whether the seeds were dried or not. All treatments significantly decreased the sprout yield of clover seeds by 13% (p < 0.05 ). If seeds were not rinsed after treatment, the germination rates of clover and broccoli seeds were reduced by 7 and 9%, respectively, and the sprout yield of alfalfa seeds was reduced by 35%. Overall, the organic acid solution was ineffective when compared with 20,000 ppm calcium hypochlorite in reducing Salmonella on sprout seeds, although the drying step after treatment could improve the treatment efficacy.
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- Title
- Grace Gospel Mission, Chicago, Illinois, ca. 1945
- Creator
- Illinois Institute of Technology
- Date
- 1943-1948
- Description
-
Photograph of the front of Grace Gospel Mission, 3138(?) South State Street. This storefront church was demolished ca. 1950. Date of...
Show morePhotograph of the front of Grace Gospel Mission, 3138(?) South State Street. This storefront church was demolished ca. 1950. Date of photograph is unknown. Date range listed is approximate.
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- Office of Communications and Marketing photographs, 1905-1999
- Title
- Grand View Blvd...Los Angeles Jan 91
- Creator
- Gordon, Robert Philip
- Date
- 1991-01
- Description
-
Watercolor painting of the intersection of an unknown street with Grand View Boulevard in Los Angeles, California.
- Collection
- Robert Philip Gordon papers, 1963-2010
- Title
- New Object
- Creator
- Zhang, He
- Title
- Ink and Pastel Drawing, undated
- Creator
- Henry, Mary Dill, 1913-2009
- Description
-
Untitled ink and pastel drawing by Mary Henry of a hand, possibly her own. Drawing is undated.
- Collection
- Mary Dill Henry Papers, 1913-2021
- Title
- Bronzeville, Chicago, Illinois, 1961
- Date
- 1961
- Description
-
Color photograph depicting the Bronzeville neighborhood in 1961. Coverage includes the 3400 south blocks of Michigan Avenue, Indiana Avenue,...
Show moreColor photograph depicting the Bronzeville neighborhood in 1961. Coverage includes the 3400 south blocks of Michigan Avenue, Indiana Avenue, Prairie Avenue, and Calumet Avenue in Chicago, Illinois. One newer white commercial building at the left is the former Chicago Defender building. Photographer unknown.
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- Bronzeville photo, 1961
- Title
- Gospel Mission Baptist Church, Chicago, Illinois, ca. 1945
- Creator
- Illinois Institute of Technology
- Date
- 1943-1948
- Description
-
Photograph of the front of the Gospel Mission Baptist Church. Date of photograph is unknown. Date range listed is approximate.
- Collection
- Office of Communications and Marketing photographs, 1905-1999
- Title
- Design on a Hill
- Creator
- Henry, Mary Dill, 1913-2009, Ziegler, William
- Description
-
Photograph of Design on a Hill (1940), a stone lithograph by Mary Dill Henry, completed as a part of her studies with Ray Bertrand....
Show morePhotograph of Design on a Hill (1940), a stone lithograph by Mary Dill Henry, completed as a part of her studies with Ray Bertrand. Handwritten inscription on verso: "Lithograph #2, 1940 Federal Art Project, litho pencil on stone. Ray Bertrand, San Francisco Art Project, was my teacher." Typewritten inscription on verso: "Mary Henry: Design on a Hill, 1940. Stone lithograph, 12" x 16". Courtesy of the artist, Freeland, WA. Photo: Wm. Ziegler." Photograph by William Ziegler. Date of photograph unknown.
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- Mary Dill Henry Papers, 1913-2021
- Title
- Federal Arts Project mosaic, Monterey, California
- Description
-
Photograph of a mural completed in Monterey, California as part of a the Federal Arts Project by Mary Dill, likely in the late 1930s,...
Show morePhotograph of a mural completed in Monterey, California as part of a the Federal Arts Project by Mary Dill, likely in the late 1930s, described on the inscription of a similar photograph as "Mary Dill's first mosaic for the Federal Art Project - the first governor's house in Monterey, Calif." Photographer unknown. Date of photograph unknown.
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- Mary Dill Henry Papers, 1913-2021
- Title
- Federal Arts Project mosaic, Monterey, California
- Description
-
Photograph of a mural completed in Monterey, California as part of a the Federal Arts Project by Mary Dill, likely in the late 1930s,...
Show morePhotograph of a mural completed in Monterey, California as part of a the Federal Arts Project by Mary Dill, likely in the late 1930s, described on the inscription of a similar photograph as "Mary Dill's first mosaic for the Federal Art Project - the first governor's house in Monterey, Calif." Photographer unknown. Date of photograph unknown.
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- Mary Dill Henry Papers, 1913-2021
- Title
- Federal Arts Project mosaic, Monterey, California
- Description
-
Photograph of a mural completed in Monterey, California as part of a the Federal Arts Project by Mary Dill, likely in the late 1930s,...
Show morePhotograph of a mural completed in Monterey, California as part of a the Federal Arts Project by Mary Dill, likely in the late 1930s, described on the inscription of a similar photograph as "Mary Dill's first mosaic for the Federal Art Project - the first governor's house in Monterey, Calif." Photographer unknown. Date of photograph unknown.
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- Mary Dill Henry Papers, 1913-2021
- Title
- Federal Arts Project mosaic, Monterey, California
- Description
-
Photograph of a mural completed in Monterey, California as part of a the Federal Arts Project by Mary Dill, likely in the late 1930s....
Show morePhotograph of a mural completed in Monterey, California as part of a the Federal Arts Project by Mary Dill, likely in the late 1930s. Inscription on verso: "Mary Dill's first mosaic for the Federal Art Project - the first governor's house in Monterey, Calif." Photographer unknown. Date of photograph unknown.
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- Mary Dill Henry Papers, 1913-2021
- Title
- Plate 5
- Creator
- Henry, Mary Dill, 1913-2009
- Date
- 1936-1939
- Description
-
Untitled ink drawing identified on recto as "Plate 5." Date of drawing is unknown. Date range listed is approximate.
- Collection
- Mary Dill Henry Papers, 1913-2021
- Title
- Ink Drawings, 1981
- Creator
- Henry, Mary Dill, 1913-2009
- Date
- 1981-02-17
- Description
-
Untitled ink drawings by Mary Henry. Inscription on verso reads "8' x 6' Feb 17 81" and also contains what appear to be mathematical...
Show moreUntitled ink drawings by Mary Henry. Inscription on verso reads "8' x 6' Feb 17 81" and also contains what appear to be mathematical calculations.
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- Mary Dill Henry Papers, 1913-2021
- Title
- LOCAL VISCOELASTIC PROPERTIES OF SOFT ANISOTROPIC FIBROUS TISSUE
- Creator
- Gallo, Nicolas Remy
- Date
- 2020
- Description
-
The current aging population, with more than 80 million "baby boomers", will present a steep medical challenge for our society in a...
Show moreThe current aging population, with more than 80 million "baby boomers", will present a steep medical challenge for our society in a foreseeable future. Half of the adults over 85 years old are predicted to be diagnosed with Alzheimer's disease by 2050. With healthcare cost reaching over 700 billion dollars in the United States, early detection of Alzheimer's disease (AD) and other co-existing neurodegenerative diseases is crucial to improve the recovery odds in patients and to decrease individual care cost. This work seeks to tackle this problem by proposing a novel computational framework toward improving the measurement of shear visco-elastic properties of brain white matter (WM), which vary with age. These measurements practically represent the effective (average) response of many cells and are typically obtained by using rheology or elastography. Although the former is direct, the latter requires the solution of an inverse problem based on a priori mechanical tissue model. The mechanical anisotropy of WM has previously not been fully explored although many inconsistencies have been reported in brain MRE experiments. To account for these inconsistencies a transversely isotropic constitutive model for the brain WM is proposed to interpret prior experiments involving 7 young and 4 older healthy men. By employing a novel inversion scheme, we report the local variation of the effective transverse and axial shear moduli in two well aligned WM structures (corpus callosum: CC; and cortical spinal tract: CST) for both the young and old cohort of healthy subjects part of the study. This work reports statistically significant changes in local regional variation of the transverse modulus across the CC for the young cohort. In the older cohort, the trend was similar yet not statistically significant. A novel candidate biomarker, the shear anisotropy metric, defined as the ratio of the transverse and axial shear moduli, found statistically significant local regional variation across the CC but not in the CST. Healthy aging was observed to decrease both transverse and axial in both CC and CST, although the variation was significant only for the CC. Finally, in an effort to understand the cause of effective transverse mechanical properties variation in WM with aging, the connection between effective and intrinsic contribution of WM cellular constituents is established. The intrinsic mechanical contributions of axons and glial matrix are separated by fitting the estimates of the effective shear moduli to a microscopic composite fiber model of myelinated axons embedded in the glial matrix. This work provides a method to establish a baseline for healthy brain mechanical properties thus promising to increase the specificity of MRE toward early diagnosis of neurodegenerative diseases. Additional oscillating disc rheology experiments with decellularized porcine myocardium, and the fabrication of a stable heterogeneous phantom matching the mechanical, diffusional and electrical properties of the WM provide foundational knowledge for due development and validation of MRE methodologies employed in other tissues.
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- Title
- Students building a snowman, Illinois Institute of Technology, Chicago, Illinois, 1980s
- Creator
- Lightfoot, Robert M., III
- Date
- 1980-1989
- Description
-
Photograph of students building a snowman on present day Siegel Field, north of S.R. Crown Hall on the Illinois Tech campus. Date of...
Show morePhotograph of students building a snowman on present day Siegel Field, north of S.R. Crown Hall on the Illinois Tech campus. Date of photograph is unknown. Date range listed is approximate.
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- Office of Communications and Marketing photographs, 1905-1999
- Title
- Spring Thing tricycle race, Illinois Institute of Technology, Chicago, Illinois, ca. 1968-1971
- Date
- 1968-1971
- Description
-
Photograph of the tricycle race during the Spring Thing. Spring Thing, sponsored by the Union Board, occurred during the fall semester,...
Show morePhotograph of the tricycle race during the Spring Thing. Spring Thing, sponsored by the Union Board, occurred during the fall semester, usually in October. The tricycle race, first held in 1968, was a highlight of the annual festivities. Photographer unknown. Date of photograph is unknown. Date range listed is approximate.
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- Office of Communications and Marketing photographs, 1905-1999
- Title
- Women's Hall (later Lewis Hall), Illinois Institute of Technology, Chicago, Illinois, ca. 1966
- Date
- 1965-1966
- Description
-
Photograph of Lewis Hall, designed by Mittelbusher & Tourtelot and constructed in 1966. The building is located at 70 East 33rd Street, and...
Show morePhotograph of Lewis Hall, designed by Mittelbusher & Tourtelot and constructed in 1966. The building is located at 70 East 33rd Street, and was known at the time of its construction as Women's Hall. Date of photograph is unknown. Date range listed is approximate. Photographer unknown.
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- Office of Communications and Marketing photographs, 1905-1999