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Title: DEVELOPMENT OF A HYDROGEL NANOPARTICLE SYSTEM FOR SUSTAINED DELIVERY OF ANIGOGENIC FACTORS FOR THERAPEUTIC NEOVASCULARIZATION
Creator: Young, Daniel A
Date: 2018, 2018-05
Description: Neovascularization requires controlled and sustained delivery of proangiogenic factors to stimulate reperfusion of ischemic tissues. Tissue...
Show moreNeovascularization requires controlled and sustained delivery of proangiogenic factors to stimulate reperfusion of ischemic tissues. Tissue engineering strategies for therapeutic neovascularization have used proangiogenic, recombinant growth factors to direct vessel development. Recently, peptides that mimic the bioactivity of growth factors have emerged as therapeutics for a variety of drug delivery applications, including therapeutic neovascularization. We designed hydrogel nanoparticles to provide sustained and tunable di usion-based release of a proangiogenic peptide, QK, and a vessel stabilizing peptide, Vasculotide (VT). These nanoparticles were combined with a tissue engineering sca old to promote tissue neovascularization. We used this nanocomposite system, utilizing peptide loaded hydrogel nanoparticles embedded in an implantable sca old, to investigate the e cacy of a dual peptide delivery strategy for therapeutic neovascularization. Inverse phase mini-emulsion polymerization (IPMP) was used to generate crosslinked poly(ethylene) glycol (PEG) hydrogel nanoparticles. We characterized the nanoparticles in terms of their swelling ratio, mesh size, surface charge ( -potential), and particle size distributions. We developed several nanoparticle formulations using various sizes and molar concentrations of PEG chains to study the e ects of crosslink density on peptide release kinetics. This resulted in the formation of nanoparticles with low and high crosslink density as well as time-dependent variations in network density due to hydrolysis. We utilized two di erent loading techniques, peptide entrapment during IPMP and peptide post-loading into the nanoparticles, and found both to be e ective via peptide loading measurements and release kinetic studies. In the case of entrapment loading, peptides were included in the aqueous precursor during nanoparticle IPMP. Peptide release kinetics were tuned through adjustments in nanoparticle crosslink density. The resulting nanoparticle crosslink density impacted both peptide loading and fractional release, as studies showed higher crosslink density nanoparticles resulted in slower peptide release. The IPMP process preserved QK secondary structure and bioactivity, as con rmed with released peptide using circular dichroism spectroscopy and a Matrigel tubulogenesis assay, respectively. In the case of the post-loading method, pristine nanoparticles were soaked in various concentrations of either QK or VT. Unlike with the entrapment loading method, crosslink density of the nanoparticles had little e ect on release kinetics. However, much higher mass amounts of peptide could be loaded using this method and thus this method was chosen for the in vivo studies. Next, we developed a hydrogel nanocomposite sca old system to sequester nanoparticles for implantation. We characterized the nanocomposite sca old system experimentally and theoretically using one-dimensional transport models of molecular di usion. We estimated peptide di usion coe cients from nanoparticles and the nanocomposites under perfect sink conditions. Importantly, we found this system capable of providing previously reported therapeutic thresholds of QK and VT. Finally, the in uence of sustained release of QK and VT on neovascularization was evaluated using a subcutaneous rat implant model. Results demonstrated statistically higher increases in perfused vessel density from peptide loaded nanocomposite sca olds as compared to sca olds where peptide was simply entrapped. These results suggest that controlled release of proangiogenic peptides from the developed nanoparticle system holds great potential for ischemic tissue repair.
Ph.D. in Biomedical Engineering, May 2018
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Title: Non-Invasive Blood Glucose Monitoring Systems (semester?), IPRO 331: Non-Invasive Blood Glucose Monitoring IPRO 331 Project Plan Sp05
Creator: Barnard, Leland, Freemire, Ben, Hsu, Yio-fan, Kieltyka, Jude, Maketiwa, Wadzanayi, Mullins, Stephen, Nazim, Mehjabeen, Oza, Veeral, Patil, Prabhav, Riaz, Quratulann, Shivakumar, Vidya, Young, Daniel
Date: 2005-05, 2005-05
Description: The objective of this IPRO project is to investigate, research, and develop methods of non-invasive blood glucose monitoring, with a view...
Show moreThe objective of this IPRO project is to investigate, research, and develop methods of non-invasive blood glucose monitoring, with a view towards continuing the work of the previous IPRO 331 team (Fall 2004).
Deliverables for IPRO 331: Non-Invasive Blood Glucose Monitoring System for the Spring 2005 semester
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Title: Non-Invasive Blood Glucose Monitoring Systems (semester?), IPRO 331: Non-Invasive Blood Glucose Monitoring IPRO 331 Final Report Sp05
Creator: Barnard, Leland, Freemire, Ben, Hsu, Yio-fan, Kieltyka, Jude, Maketiwa, Wadzanayi, Mullins, Stephen, Nazim, Mehjabeen, Oza, Veeral, Patil, Prabhav, Riaz, Quratulann, Shivakumar, Vidya, Young, Daniel
Date: 2005-05, 2005-05
Description: The objective of this IPRO project is to investigate, research, and develop methods of non-invasive blood glucose monitoring, with a view...
Show moreThe objective of this IPRO project is to investigate, research, and develop methods of non-invasive blood glucose monitoring, with a view towards continuing the work of the previous IPRO 331 team (Fall 2004).
Deliverables for IPRO 331: Non-Invasive Blood Glucose Monitoring System for the Spring 2005 semester
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Title: Non-Invasive Blood Glucose Monitoring Systems (semester?), IPRO 331: Non-Invasive Blood Glucose Monitoring IPRO 331 Midterm Report Sp05
Creator: Barnard, Leland, Freemire, Ben, Hsu, Yio-fan, Kieltyka, Jude, Maketiwa, Wadzanayi, Mullins, Stephen, Nazim, Mehjabeen, Oza, Veeral, Patil, Prabhav, Riaz, Quratulann, Shivakumar, Vidya, Young, Daniel
Date: 2005-05, 2005-05
Description: The objective of this IPRO project is to investigate, research, and develop methods of non-invasive blood glucose monitoring, with a view...
Show moreThe objective of this IPRO project is to investigate, research, and develop methods of non-invasive blood glucose monitoring, with a view towards continuing the work of the previous IPRO 331 team (Fall 2004).
Deliverables for IPRO 331: Non-Invasive Blood Glucose Monitoring System for the Spring 2005 semester
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Title: Non-Invasive Blood Glucose Monitoring Systems (semester?), IPRO 331: Non-Invasive Blood Glucose Monitoring IPRO 331 Abstract Sp05
Creator: Barnard, Leland, Freemire, Ben, Hsu, Yio-fan, Kieltyka, Jude, Maketiwa, Wadzanayi, Mullins, Stephen, Nazim, Mehjabeen, Oza, Veeral, Patil, Prabhav, Riaz, Quratulann, Shivakumar, Vidya, Young, Daniel
Date: 2005-05, 2005-05
Description: The objective of this IPRO project is to investigate, research, and develop methods of non-invasive blood glucose monitoring, with a view...
Show moreThe objective of this IPRO project is to investigate, research, and develop methods of non-invasive blood glucose monitoring, with a view towards continuing the work of the previous IPRO 331 team (Fall 2004).
Deliverables for IPRO 331: Non-Invasive Blood Glucose Monitoring System for the Spring 2005 semester
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Title: Non-Invasive Blood Glucose Monitoring Systems (semester?), IPRO 331
Creator: Barnard, Leland, Freemire, Ben, Hsu, Yio-fan, Kieltyka, Jude, Maketiwa, Wadzanayi, Mullins, Stephen, Nazim, Mehjabeen, Oza, Veeral, Patil, Prabhav, Riaz, Quratulann, Shivakumar, Vidya, Young, Daniel
Date: 2005-05, 2005-05
Description: The objective of this IPRO project is to investigate, research, and develop methods of non-invasive blood glucose monitoring, with a view...
Show moreThe objective of this IPRO project is to investigate, research, and develop methods of non-invasive blood glucose monitoring, with a view towards continuing the work of the previous IPRO 331 team (Fall 2004).
Deliverables for IPRO 331: Non-Invasive Blood Glucose Monitoring System for the Spring 2005 semester
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Title: Low-Cost Water Purification System Design & analysis for Applications in Mexico and the US (semester?), IPRO 355: KlarAqua IPRO 355 Brochure Sp06
Creator: Aderangi, Vince, Gomez Balboa, Adrian, Craig, Seun, Gilliam, Amanda, Grimmer, Laura, Jimenez, Esmeralda, Martis, Isaac, Nambiar, Abhilash R., Nguyen, Uyen, Nuanual, Ruckfa Mek, Ogunsanwo, Olumide, Primozic, Chris, Ramirez, Eugenio, Young, Daniel
Date: 2006-05, 2006-05
Description: The main objective of the IPRO is to provide direct health services to low-income socieites, specifically Mexico, by the design of a water...
Show moreThe main objective of the IPRO is to provide direct health services to low-income socieites, specifically Mexico, by the design of a water purification filter. The IPRO will design a clay based filter and a business plan with the aim of introduction into these societies. The filter should be simple to use, affordable, simple to setup, easy to maintain and should be adaptable to a variety of every day househould activities such as drinking, bathing and washing.
Deliverables for IPRO 355: Low-Cost Water Purification System Design & analysis for Applications in Mexico and the US for the Spring 2006 semester
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Title: Low-Cost Water Purification System Design & analysis for Applications in Mexico and the US (semester?), IPRO 355: KlarAqua IPRO 355 IPRO Day Presentation Sp06
Creator: Aderangi, Vince, Gomez Balboa, Adrian, Craig, Seun, Gilliam, Amanda, Grimmer, Laura, Jimenez, Esmeralda, Martis, Isaac, Nambiar, Abhilash R., Nguyen, Uyen, Nuanual, Ruckfa Mek, Ogunsanwo, Olumide, Primozic, Chris, Ramirez, Eugenio, Young, Daniel
Date: 2006-05, 2006-05
Description: The main objective of the IPRO is to provide direct health services to low-income socieites, specifically Mexico, by the design of a water...
Show moreThe main objective of the IPRO is to provide direct health services to low-income socieites, specifically Mexico, by the design of a water purification filter. The IPRO will design a clay based filter and a business plan with the aim of introduction into these societies. The filter should be simple to use, affordable, simple to setup, easy to maintain and should be adaptable to a variety of every day househould activities such as drinking, bathing and washing.
Deliverables for IPRO 355: Low-Cost Water Purification System Design & analysis for Applications in Mexico and the US for the Spring 2006 semester
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Title: Low-Cost Water Purification System Design & analysis for Applications in Mexico and the US (semester?), IPRO 355
Creator: Aderangi, Vince, Gomez Balboa, Adrian, Craig, Seun, Gilliam, Amanda, Grimmer, Laura, Jimenez, Esmeralda, Martis, Isaac, Nambiar, Abhilash R., Nguyen, Uyen, Nuanual, Ruckfa Mek, Ogunsanwo, Olumide, Primozic, Chris, Ramirez, Eugenio, Young, Daniel
Date: 2006-05, 2006-05
Description: The main objective of the IPRO is to provide direct health services to low-income socieites, specifically Mexico, by the design of a water...
Show moreThe main objective of the IPRO is to provide direct health services to low-income socieites, specifically Mexico, by the design of a water purification filter. The IPRO will design a clay based filter and a business plan with the aim of introduction into these societies. The filter should be simple to use, affordable, simple to setup, easy to maintain and should be adaptable to a variety of every day househould activities such as drinking, bathing and washing.
Deliverables for IPRO 355: Low-Cost Water Purification System Design & analysis for Applications in Mexico and the US for the Spring 2006 semester
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Title: Low-Cost Water Purification System Design & analysis for Applications in Mexico and the US (semester?), IPRO 355: KlarAqua IPRO 355 Project Plan Sp06
Creator: Aderangi, Vince, Gomez Balboa, Adrian, Craig, Seun, Gilliam, Amanda, Grimmer, Laura, Jimenez, Esmeralda, Martis, Isaac, Nambiar, Abhilash R., Nguyen, Uyen, Nuanual, Ruckfa Mek, Ogunsanwo, Olumide, Primozic, Chris, Ramirez, Eugenio, Young, Daniel
Date: 2006-05, 2006-05
Description: The main objective of the IPRO is to provide direct health services to low-income socieites, specifically Mexico, by the design of a water...
Show moreThe main objective of the IPRO is to provide direct health services to low-income socieites, specifically Mexico, by the design of a water purification filter. The IPRO will design a clay based filter and a business plan with the aim of introduction into these societies. The filter should be simple to use, affordable, simple to setup, easy to maintain and should be adaptable to a variety of every day househould activities such as drinking, bathing and washing.
Deliverables for IPRO 355: Low-Cost Water Purification System Design & analysis for Applications in Mexico and the US for the Spring 2006 semester
Show less

Title: Low-Cost Water Purification System Design & analysis for Applications in Mexico and the US (semester?), IPRO 355: KlarAqua IPRO 355 Abstract Sp06
Creator: Aderangi, Vince, Gomez Balboa, Adrian, Craig, Seun, Gilliam, Amanda, Grimmer, Laura, Jimenez, Esmeralda, Martis, Isaac, Nambiar, Abhilash R., Nguyen, Uyen, Nuanual, Ruckfa Mek, Ogunsanwo, Olumide, Primozic, Chris, Ramirez, Eugenio, Young, Daniel
Date: 2006-05, 2006-05
Description: The main objective of the IPRO is to provide direct health services to low-income socieites, specifically Mexico, by the design of a water...
Show moreThe main objective of the IPRO is to provide direct health services to low-income socieites, specifically Mexico, by the design of a water purification filter. The IPRO will design a clay based filter and a business plan with the aim of introduction into these societies. The filter should be simple to use, affordable, simple to setup, easy to maintain and should be adaptable to a variety of every day househould activities such as drinking, bathing and washing.
Deliverables for IPRO 355: Low-Cost Water Purification System Design & analysis for Applications in Mexico and the US for the Spring 2006 semester
Show less

Title: Low-Cost Water Purification System Design & analysis for Applications in Mexico and the US (semester?), IPRO 355: KlarAqua IPRO 355 Final Report Sp06
Creator: Aderangi, Vince, Gomez Balboa, Adrian, Craig, Seun, Gilliam, Amanda, Grimmer, Laura, Jimenez, Esmeralda, Martis, Isaac, Nambiar, Abhilash R., Nguyen, Uyen, Nuanual, Ruckfa Mek, Ogunsanwo, Olumide, Primozic, Chris, Ramirez, Eugenio, Young, Daniel
Date: 2006-05, 2006-05
Description: The main objective of the IPRO is to provide direct health services to low-income socieites, specifically Mexico, by the design of a water...
Show moreThe main objective of the IPRO is to provide direct health services to low-income socieites, specifically Mexico, by the design of a water purification filter. The IPRO will design a clay based filter and a business plan with the aim of introduction into these societies. The filter should be simple to use, affordable, simple to setup, easy to maintain and should be adaptable to a variety of every day househould activities such as drinking, bathing and washing.
Deliverables for IPRO 355: Low-Cost Water Purification System Design & analysis for Applications in Mexico and the US for the Spring 2006 semester
Show less

Title: Low-Cost Water Purification System Design & analysis for Applications in Mexico and the US (semester?), IPRO 355: KlarAqua IPRO 355 Poster Sp06
Creator: Aderangi, Vince, Gomez Balboa, Adrian, Craig, Seun, Gilliam, Amanda, Grimmer, Laura, Jimenez, Esmeralda, Martis, Isaac, Nambiar, Abhilash R., Nguyen, Uyen, Nuanual, Ruckfa Mek, Ogunsanwo, Olumide, Primozic, Chris, Ramirez, Eugenio, Young, Daniel
Date: 2006-05, 2006-05
Description: The main objective of the IPRO is to provide direct health services to low-income socieites, specifically Mexico, by the design of a water...
Show moreThe main objective of the IPRO is to provide direct health services to low-income socieites, specifically Mexico, by the design of a water purification filter. The IPRO will design a clay based filter and a business plan with the aim of introduction into these societies. The filter should be simple to use, affordable, simple to setup, easy to maintain and should be adaptable to a variety of every day househould activities such as drinking, bathing and washing.
Deliverables for IPRO 355: Low-Cost Water Purification System Design & analysis for Applications in Mexico and the US for the Spring 2006 semester
Show less

Title: Low-Cost Water Purification System Design & analysis for Applications in Mexico and the US (semester?), IPRO 355: KlarAqua IPRO 355 Midterm Report Sp06
Creator: Aderangi, Vince, Gomez Balboa, Adrian, Craig, Seun, Gilliam, Amanda, Grimmer, Laura, Jimenez, Esmeralda, Martis, Isaac, Nambiar, Abhilash R., Nguyen, Uyen, Nuanual, Ruckfa Mek, Ogunsanwo, Olumide, Primozic, Chris, Ramirez, Eugenio, Young, Daniel
Date: 2006-05, 2006-05
Description: The main objective of the IPRO is to provide direct health services to low-income socieites, specifically Mexico, by the design of a water...
Show moreThe main objective of the IPRO is to provide direct health services to low-income socieites, specifically Mexico, by the design of a water purification filter. The IPRO will design a clay based filter and a business plan with the aim of introduction into these societies. The filter should be simple to use, affordable, simple to setup, easy to maintain and should be adaptable to a variety of every day househould activities such as drinking, bathing and washing.
Deliverables for IPRO 355: Low-Cost Water Purification System Design & analysis for Applications in Mexico and the US for the Spring 2006 semester
Show less

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