Cyclic adenosine monophosphate (cAMP, cyclic AMP or 3'-5'-cyclic adenosine monophosphate) receptor protein (abbreviated as CRP), also known as... Show moreCyclic adenosine monophosphate (cAMP, cyclic AMP or 3'-5'-cyclic adenosine monophosphate) receptor protein (abbreviated as CRP), also known as catabolite gene activator protein, is one type of regulatory protein in bacteria. It acts as a secondary messenger in many intracellular pathways, especially the signal transduction by binding cAMP, which allows the protein to bind DNA sequence in the promoters. Fumarate/nitrate reduction transcriptional regulator, in short Fnr, is another signal transduction molecule which transfers from oxygen status to nifL (Nitrogen fixing bacteria L) protein. Both Crp and Fnr can be grouped into a Crp-Fnr family of transcription factors in many bacteria strains. In this study, we identified Crp-Fnr family genes from different bacteria genomes. Using the Crp-Fnr gene sequences we reconstructed phylogenetic trees for 250 select bacterial genomes which contains at least one Crp-Fnr family gene. We also selected representative Escherichia coli and Salmonella enteriaca genomes for phylogenetic analysis. The circular maps for the four select bacterial genus, Listeria, Salmonella, Escherichia and Brucella were created to demonstrate the genomic locations of Crp-Fnr family genes. Our results showed that the location of Crp-Fnr family transcription factor genes are syntenic amongdifferent bacteria bacterial genomes, indicating their early ancestry acquisition prior to the speciation events. M.S. in Biology, May 2012 Show less
