This data set supports the process development work described in the associated manuscript, capturing the transition from bench-scale synthesis to small-scale process development of hydrogel... Show moreThis data set supports the process development work described in the associated manuscript, capturing the transition from bench-scale synthesis to small-scale process development of hydrogel nanoparticles. It includes the raw and processed quantitative data for experimental variables tested during this scale-down effort. Additionally, the data set provides the original red-green channel versions of fluorescence microscopy images used in cell studies. In the manuscript, these images were adjusted to be accessible for readers with red-green color vision deficiency; here, the unmodified channel versions are made available to ensure scientific transparency and reproducibility. Show less