Title: Data Repository for "Small-Scale Process Engineering for Inverse Phase Miniemulsion Polymerization of Hydrogel Nanoparticles for Therapeutic Applications"

Authors: Ruth Negru, Fernando T.P. Borges, Georgia Papavasiliou, Fouad Teymour, Marcella K. Vaicik

Corresponding Author: Marcella K. Vaicik (mvaicik@illinoistech.edu)


1. Overview 
This repository contains the raw data, processed data, analysis files, and images analyzed for quantification associated with the publication listed above. All data correspond to experiments described in the main manuscript and Supporting Information. 


2. Folder Structure
Data are first organized by either miniemulsion polymerization process steps or cell studies. Then, data are organized by figure to correspond directly with the main manuscript. Each sub-folder (e.g. Figure 1C Data) contains all raw NanoSight output reports, raw .csv data files from temperature probe readings and mass scale readings, and the GraphPad Prism file used to analyze those data and generate the corresponding figure panel. 

For each folder, PDF files are instrument-generated Nanosight reports (labeled by Figure, experimental condition, and replicate), .csv files contain numbers generated with measurement instruments (temperature and mass), and Prism files contain the statistical analyses and plotted results used in the manuscript. All reported values were derived directly from the instruments and instrument summary outputs.

This file Data_Summary_AllFigures.xlsx contains the raw data and calculated mean ± standard deviation values for all figures presented in the manuscript. Each sheet is labeled according to the corresponding figure (e.g. "Figure 1C", "Figure 4" etc.). These values were obtained by averaging replicate-level results from mass, temperature, Image J, and Nanosight PDF outputs. The calculated mean and standard deviation values in this file are the same numerical results reported in the main text and figure captions. 

3. Software and Version Information
-GraphPad Version 10.4.1 (GraphPad Software, LLC)
-Excel Version 16.92 (24120731) (Microsoft 365)
-NanoSight NTA software v3.0 and v3.44

4. Notes for Reproducibility
All raw data are reported exactly as collected. Each .prism file includes the corresponding statistical tests and plots, matching data within file Data_Summary_AllFigures.xlsx as gathered from raw data files. 
Users may open these files in GraphPad Prism to view test parameters and graph formatting. Figures generated in Prism were exported directly. 

5. Contact
For questions regarding data interpretation or analysis details, please contact Marcella Vaicik (mvaicik@illinoistech.edu) and Ruth Negru (rnegru@hawk.illinoistech.edu).