Botanical/herbal teas may contain antimicrobial compounds that will affect the survival and persistence of the native as well as contaminant... Show moreBotanical/herbal teas may contain antimicrobial compounds that will affect the survival and persistence of the native as well as contaminant microflora. Although Salmonella are known for their ability to survive in low water activity environments, their survival on dry botanical/herbal teas has not been examined. The purpose of this work is to determine the survival of Salmonella on three types of teas, chamomile peppermint, and green tea under different storage conditions. In addition, the ability of Salmonella to grow in tea brewed with contaminated product was examined. A cocktail of four Salmonella serovars was inoculated onto 1 g samples of chamomile, peppermint, and green tea to give a starting cell population of approximately 10 log cfu/g. The inoculum was allowed to dry prior to storage at 25 °C and 35 °C and at low (<30% RH) and high (> 90% RH) humidity levels. At different time intervals up to 6 months, triplicate samples at each condition were evaluated for surviving Salmonella populations. In addition, samples were used to produce a low-temperature (sun) brewed tea and inoculated. Growth was assessed after 24 hr. Under the four storage conditions tested, survival followed the order 25°C/low RH > 35°C/low RH > 25°C/high RH > 35°C/high RH. Salmonella losses at 25°C/low RH occurred primarily during drying, after which populations showed little declines over 6 months. In contrast Salmonella decreased below detection after 45 days at 35°C and high RH in all teas tested. All teas tested supported the growth of Salmonella after brewing. Storage conditions significantly impact the survival of Salmonella in dried herbal teas. If Salmonella survives after storage, it may also survive and grow after a home brewing process. M.S. in Food Safety and Technology, May 2013 Show less