The synchronous indirect flight muscle (DLM1) of the Hawk moth, Manduca Sexta, has similarities to cardiac striated muscle in its... Show moreThe synchronous indirect flight muscle (DLM1) of the Hawk moth, Manduca Sexta, has similarities to cardiac striated muscle in its physiological properties. In particular, both operate in vivo on the so-called ascending limb of the length-tension curve. The length-tension curve is a classical experiment to study the physiological properties of muscle. The length tension curve of DLM1 of Manduca sexta has previously been reported with a descending limb that is steeper than what would be expected given likely dimensions for the thick and thin filaments. Excessive rundown of the muscle preparations is a likely cause of these observations. Factors caused muscle rundown are many, such as the time allowed for the muscle to relax, the time for muscle spends contracting or the time spent on sarcomere length adjusting. Insights into the factors mentioned above were obtained by conducting a series of experiments designed to systematically explore the causes of rundown. These included Constant Sarcomere Length Experiments, Reciprocating Stretch Experiments and Back to SL 3.25 μm Experiment. Then a modified protocol for carrying out the length-tension experiment was developed based on these findings. A new length-tension curve was plotted and shows a shape closer to what might be expected theoretically. The force went to zero at about SL 5.7μm. This result is constant with other measurements of the length of the myofilaments. Finally, the lattice spacing experiment was carried out to figure out how the interfilament lattice spacing changes across the SL growing. Result shows that the lattice spacing did not change much over the plateau region of the length-tension curve (SL 2.9- 3.1) but increased substantially as SL decreases further in the ascending limb of the length tension curve. M.S. in Biology, May 2016 Show less
