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MOLECULAR MECHANISMS UNDERLYING SALMONELLA SURVIVAL ON SURFACE OF SELECTED NUTS AND FRUITS
Title
MOLECULAR MECHANISMS UNDERLYING SALMONELLA SURVIVAL ON SURFACE OF SELECTED NUTS AND FRUITS
Creator
Li, Ye
Date
2017, 2017-07
Description
Salmonella enterica is the leading cause of bacterial foodborne illness in the United States. In recent years, S. enterica has been frequently...
Show moreSalmonella enterica is the leading cause of bacterial foodborne illness in the United States. In recent years, S. enterica has been frequently linked to foodborne outbreaks associated with nuts and fruits; however, the underlying mechanisms of such association have not been fully understood. In the first part of this study, we evaluated the impact of various environmental factors and food surface attributes on the attachment and survival of five S. enterica strains representing serotypes Typhimurium, Enteritidis, Montevideo, Mbandanka, and Braenderup on three different raw nuts (i.e. black peppers, almonds and hazelnuts) and two different S. enterica strains including serotypes Typhimurium and Enteritidis on two fresh fruits (i.e. grape tomatoes and cantaloupes) under storage conditions relevant to industrial practice. We observed significant inter-strain variations in S. enterica survival on nut and fruit surface. A direct correlation was found between the nut and fruit surface roughness and S. enterica attachment and survival. Lower relative humidity (20%) and higher storage temperature (25oC) resulted in significant S. enterica reduction on nut shells. Lower storage temperature at 4oC significantly reduced S. enterica population on grape tomatoes. In the second part of this study, we used a newly-developed transposon mutagenesis library in S. enterica serotype Enteritidis genome and highthroughput sequencing analysis to identify genes with potential roles in S. enterica attachment to and survival on almonds and grape tomatoes. A total of 336 and 210 S. enterica genes displayed significant selection on almonds and grape tomatoes over a 7-d storage period at 25oC (p<0.05), respectively. Our results suggest that various food attributes, environmental factors as well as bacterial determinants collectively contribute to the survival and persistence of S. enterica on nuts and fruits, providing new data for future development of knowledge-based intervention strategies.
Ph.D. in Biology, July 2017
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SYSTEMATIC CONSTRUCTION OF SALMONELLA ENTERITIDIS-SPECIFIC GENE DELETION MUTATIONS
Title
SYSTEMATIC CONSTRUCTION OF SALMONELLA ENTERITIDIS-SPECIFIC GENE DELETION MUTATIONS
Creator
Li, Ye
Date
2013, 2013-12
Description
Salmonella enterica serovar Enteritidis infection is one of the major foodborne illnesses associated with egg, cattle and poultry products....
Show moreSalmonella enterica serovar Enteritidis infection is one of the major foodborne illnesses associated with egg, cattle and poultry products. Genes which are specific to S. Enteritidis may play an important functional role in the pathogens’ ability to survive and persist in these food products, and thus cause human foodborne outbreaks. Acquiring more knowledge on how this bacterium is capable of survival will aid in developing strategies to minimize its existence in the Nation’s food supply. In this study, we constructed S. Enteritidis-specific gene deletion mutants for use in subsequent functional studies. Out of more than 200 S. Enteritidis specific genes, 46 genes were selected for deletion in S. Enteritidis PT4. These 46 genes were selected because they are absent in both S. Typhimurium and S. Typhi based on percent identity scores retrieved from standalone BLAST software analysis. The deletion mutants were constructed using the lambda red recombinase method and a PCR product containing unique barcodes. This library of uniquely barcoded mutants will be useful in determining survival and persistence of this organism both in nature and in vivo.
M.S. in Food Safety and Technology, December 2013
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