Cholera is a severe disease that causes devastating diarrhea when Vibrio cholerae infects the human intestine. Once the bacterium gets the... Show moreCholera is a severe disease that causes devastating diarrhea when Vibrio cholerae infects the human intestine. Once the bacterium gets the access into the intestinal cell, adenosine diphosphate (ADP)- ribosylation of the human signaling protein Gs!!is catalyzed by the cholera toxin A1 subunit (CTA1). According to the previous researches, this reaction is activated allosterically by a GTP-bound human ADP-ribosylation factor six (ARF6). Arginine is one of the substrates for cholera toxin A1 subunit. In the thesis, the crystal structure of agmatine, a L-arginine analogue, bound to the protein complex of the mutant CTA1 with ARF6-GTP reveals the possibility of how arginine might interact with the complex. M.S. in Molecular Biochemistry and Biophysics, July 2012 Show less
