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- Title
- GROWTH KINETICS OF SALMONELLA ENTERICA DURING REHYDRATION OF DEHYDRATED PLANT FOODS AND SUBSEQUENT STORAGE
- Creator
- Ren, Yuying
- Date
- 2020
- Description
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Dehydrated plant foods have low water activities and do not support the growth of pathogenic bacteria like Salmonella enterica. Once...
Show moreDehydrated plant foods have low water activities and do not support the growth of pathogenic bacteria like Salmonella enterica. Once rehydration, the water activities will increase to > 0.92, and along with their neutral pHs, plant foods may be able to support the growth of S. enterica. Therefore, product assessments are required to determine the extent to which these products support growth of S. enterica. The purpose of this study was to determine the growth kinetics of S. enterica during rehydration with 5 or 25 °C water, and subsequent storage of dehydrated potatoes, carrots, and onions at 5, 10, and 25 °C. Fresh plant foods were dehydrated at 60°C (140°F) for 24 h. Dehydrated plant foods were inoculated with 4 log CFU/g of a 4-strain cocktail of S. enterica and dried for 24 h. Samples were rehydrated using 4-volumes of 5 or 25 °C water for 24 h. During rehydration, 30 g of sample was removed and drained for 10 min. Ninety mL of BPB was added to triplicate 10-g samples. Serial dilutions of the homogenate were plated onto TSA overlaid with XLD agar for enumeration of S. enterica. After 24 h rehydration, the remaining samples were drained and stored in containers at 5, 10, and 25°C for 7 d. S. enterica was enumerated at 1, 3, 5, and 7 d. Three independent trials were conducted. Growth kinetics were determined using DMFit and data were statistically analyzed using Student’s t-test (α=0.05). Overall, the growth rates of S. enterica when 5 °C water was used for rehydration were higher than when 25 °C water was used for potatoes and carrots. The highest growth rate of S. enterica was 3.74 log CFU/g per d on potatoes, leading to a 1 log CFU/g increase in S. enterica after only 0.27 d (16 h) which occurred during storage at 25 ℃ after 5℃ water rehydration. The highest growth rate on carrots was 1.98 log CFU/g per d (requiring only 0.51 d to increase 1 log CFU/g) when rehydrated with 5℃ water and stored at 25 ℃. The growth rates were the lowest during the storage of rehydrated onions. S. enterica required 12.5 d to increase 1 log CFU/g (the growth rate was 0.61 log CFU/g per d) when the onions were rehydrated with 25 ℃ water and stored at 25 ℃. The results of this study determined that S. enterica could survive and grow in dehydrated plant foods during rehydration and storage, highlighting the need for product assessments for these types of foods.
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- Title
- Analysis of High-Fidelity Experiments and Simulations of the Flow in Simplified Urban Environments
- Creator
- Stuck, Maxime
- Date
- 2020
- Description
-
The mean flow and turbulence statistics of the flow through a simplified urban environment, which is an active research area in order to improve...
Show moreThe mean flow and turbulence statistics of the flow through a simplified urban environment, which is an active research area in order to improve the knowledge of turbulent flow in cities, is investigated. This is useful for civil engineering, pedestrian comfort and for health concerns caused by pollutant spreading. In this work, we provide analysis of the turbulence statistics obtained both from highly-quality stereoscopic particle image-velocimetry (SPIV) measurements (from Monnier et al.) and well-resolved large eddy simulations (LES) by Torres et al. A detailed comparison of both databases reveals the impact of the geometry of the urban array on the flow characteristics and provides for a good description of the turbulent features of the flow around a simplified urban environment. The most prominent features of this complex flow include coherent vortical structures such as the so-called arch vortex, the horseshoe vortex, or the roof vortex. These structures of the flow have been identified by an analysis of the turbulence statistics. The influence of the geometry of the urban environment (and particularly the street width and the building height) on the overall flow behavior has also been studied.
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- Title
- IMPACT OF DATA SHAPE, FIDELITY, AND INTER-OBSERVER REPRODUCIBILITY ON CARDIAC MAGNETIC RESONANCE IMAGE PIPELINES
- Creator
- Obioma, Blessing Ngozi
- Date
- 2020
- Description
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Artificial Intelligence (AI) holds a great promise in the healthcare. It provides a variety of advantages with its application in clinical...
Show moreArtificial Intelligence (AI) holds a great promise in the healthcare. It provides a variety of advantages with its application in clinical diagnosis, disease prediction, and treatment, with such interests intensifying in the medical image field. AI can automate various cumbersome data processing techniques in medical imaging such as segmentation of left ventricular chambers and image-based classification of diseases. However, full clinical implementation and adaptation of emerging AI-based tools face challenges due to the inherently opaque nature of such AI algorithms based on Deep Neural Networks (DNN), for which computer-trained bias is not only difficult to detect by physician users but is also difficult to safely design in software development. In this work, we examine AI application in Cardiac Magnetic Resonance (CMR) using an automated image classification task, and thereby propose an AI quality control framework design that differentially evaluates the black-box DNN via carefully prepared input data with shape and fidelity variations to probe system responses to these variations. Two variants of the Visual Geometric Graphics with 19 neural layers (VGG19) was used for classification, with a total of 60,000 CMR images. Findings from this work provides insights on the importance of quality training data preparation and demonstrates the importance of data shape variability. It also provides gateway for computation performance optimization in training and validation time.
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- Title
- LOW-COVERAGE GENOMES AS AN EFFECTIVE AND ECONOMICAL APPROACH FOR LEPIDOPTERAN MICROSATELLITE ISOLATION
- Creator
- Liang, Huijia
- Date
- 2020
- Description
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This study aimed to verify that whether a low-coverage genome can work as an effective approach to isolate Lepidopteran microsatellites. As...
Show moreThis study aimed to verify that whether a low-coverage genome can work as an effective approach to isolate Lepidopteran microsatellites. As microsatellites are useful tool to study population genetics, and there are many Lepidopteran agriculture pests which can cause huge economic damages every year, additionally, Lepidoptera have abundant similar flanking sequences making it difficult to develop reliable microsatellites. However, there are not enough published genomes of Lepidoptera species. If low-coverage Lepidopteran genomes can be used to isolate reliable microsatellites, the low-coverage genomes would be an effective and economical approach for microsatellites isolation, because low-coverage genome sequencing is much cheaper and less time-consuming than the published genome sequencing.
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- Title
- Associations between subjective cognitive decline, neurodegeneration, and vascular neuroimaging markers: Findings from a multiethnic cohort
- Creator
- Gonzalez, Christopher
- Date
- 2023
- Description
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Mounting evidence suggests that subjective cognitive decline (SCD) may provide a unique target to identify the earliest changes in cognitive...
Show moreMounting evidence suggests that subjective cognitive decline (SCD) may provide a unique target to identify the earliest changes in cognitive function in Alzheimer’s disease (AD). In addition, vascular-related risk factors are also linked to increase the risk of clinical expression of AD, and independently increase the risk for vascular dementia (VaD). However, most investigations have not explored SCD across a multiethnic population. The study investigated 1) the associations between white matter hyperintensities (WMH) and targeted neuroimaging AD markers (hippocampal volume, cortical thickness of AD regions) with SCD amongst a multiethnic cohort, and 2) whether race moderated the relationship between them. A total of 871 older adults ages from 62-96 years old with a mean age of 74.48 (SD = 6.11), mean education of 12.79 years (SD = 4.53), and with 62% identifying as female were recruited from preexisting data from the Washington Heights Inwood Columbia Aging Project (WHICAP). Linear regression model revealed a significant association between WMH and both AD targeted neuroimaging markers across the total sample. Secondary analyses revealed that race did not moderate the relationship between WMH and AD cortical thickness with SCD but did in fact moderate the relationship between hippocampal volume and SCD. Results suggest that cultural biological differences exist in the Hispanic/Latine individuals compared to non-Hispanic White and non-Hispanic Black individuals.
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- Title
- Students International House
- Creator
- Peterhans, Brigitte 1928-2021
- Date
- 1961
- Title
- Sex and Architecture
- Creator
- Nekkanti, Hashika C.
- Date
- 2023
- Description
-
This thesis delves into the fascinating and intricate connection between sexual behaviour and architecture, offering a comprehensive...
Show moreThis thesis delves into the fascinating and intricate connection between sexual behaviour and architecture, offering a comprehensive exploration of the multifaceted factors that shape human sexuality within the context of built environments. The research is divided into six chapters, each providing a distinct perspective on the complex relationship between sexuality and the spaces we inhabit. Chapter 1 serves as an introduction, setting the stage for the study and highlighting the importance of understanding how architectural design and spatial arrangements can influence and be influenced by human sexual behaviours. Chapter 2, "Milieu and Sexed Bodies," delves into the impact of environmental stimuli on sexual arousal. By examining how architectural elements, aesthetics, and spatial configurations can elicit eroticism and arousal, this chapter sheds light on the subtle ways in which built environments interact with our intimate desires. Furthermore, it delves into the psychological aspects of fetishism and voyeurism, exploring how these sexual behaviours find expression within architectural settings. Chapter 3, titled "Sexual Behaviour," delves into the profound influence of history on human sexuality. It examines how cultural norms, religious beliefs, and social backgrounds have shaped and continue to shape sexual attitudes and behaviours across different societies and time periods. The chapter also explores the concept of polymorphous behaviour, emphasizing how architecture can create spaces that cater to diverse expressions of human sexuality. Additionally, it investigates the impact of queer spaces, highlighting the significance of inclusive design in fostering a sense of belonging and acceptance for individuals with diverse sexual identities. Chapter 4 turns its attention to "Mediated Sexuality," offering a historical analysis of how various forms of media have played a pivotal role in shaping sexual attitudes and behaviours. From ancient art to contemporary pornography, this chapter explores how media representations have influenced perceptions of sexuality, and it examines the emergence of pornomodernism, a cultural phenomenon impacting both media and architectural expressions of eroticism. Moreover, it investigates how architecture has incorporated and responded to the eroticized spaces depicted in media. Chapter 5 focuses on the "Coital Environment," a fascinating aspect of architecture that intertwines with sexual behaviour. This chapter delves into the realm of sex tourism, examining how certain destinations and spaces have become associated with sexual encounters. It analyses the design and function of dedicated places for sex within architectural contexts, providing insights into the factors that influence the creation and acceptance of such spaces in different societies. Finally, Chapter 6 presents a comprehensive "Conclusion," synthesizing the key findings from the preceding chapters. It emphasizes the critical role that architecture plays in shaping human sexual behaviours and highlights the social and cultural implications of design decisions in built environments. The chapter concludes with reflections on the significance of creating inclusive and supportive spaces that celebrate and embrace diverse sexual identities and experiences, paving the way for more empathetic and human-centric architectural design in the future. In conclusion, this thesis offers a profound exploration of sexual behaviour and architecture, shedding light on the intricate interplay of environmental stimuli, historical influences, and mediated sexuality in shaping our intimate experiences within the spaces we inhabit. It serves as a valuable resource for architects, urban planners, social scientists, and anyone interested in creating environments that foster a positive and inclusive approach to human sexuality.
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