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Title: CHARACTERIZATION OF ASXL2 AS AN INTRINSICALLY DISORDERED PROTEIN
Creator: Chen, Po-tao
Date: 2013, 2013-12
Description: During development and differentiation, the transcription regulation plays an important rule. The Polycomb Group (PcG) and the Trithorax Group...
Show moreDuring development and differentiation, the transcription regulation plays an important rule. The Polycomb Group (PcG) and the Trithorax Group (TrxG) proteins are two protein families; they act by forming multiprotein complexes that regulate transcription by modifying chromatin configurations through histone methylation. The establishment or the maintenance mistakes of the correct chromatin configuration have been connected to several diseases, including leukemia and muscular dystrophy. The activities of PcG and TrxG proteins are regulated by a special group of proteins known as Enhancers of trithorax and Polycomb (ETP). The Additional sex combs-like 2 (Asxl2) protein, which is expressed from the gene called additional sex combs like 2 (Drosophila) (Asxl2), belongs to the Enhancer of trithorax and Polycomb group (ETP). The Asxl2 protein is highly expressed in heart during both development and in adult life. In a genetic study, the Asxl2-/- mice either died as neonates with congenital heart abnormalities or displayed progressive deterioration of ventricular function. Although Asxl2 protein is important, there have been no significant studies of the Asxl2 protein’s structure. Protein functions are determined to a considerable degree by structure, so the structural studies will provide valuable information on Asxl2. Failures of protein folding into native structure generally produce inactive or toxic proteins. Misfolded proteins are believed to be the causes of some neurodegenerative diseases, including Alzheimer’s and Parkinson's diseases. ix Through investigation in silico, it is known that the Asxl2 protein contains a large native unstructured region, and this result might indicate the Asxl2 is an intrinsically disordered protein (IDP). Most IDPs are known to have regulatory roles. Owing to their lack of folded structure, the intrinsically disordered proteins display a flexible and random-coil-like conformation under physiological conditions, and have several unique features. This suggests that distinctive methods will be required to characterize their structure. In order to understand the structure of Asxl2, we have expressed and purified an Asxl2 N-terminal truncation that contains a large native unstructured region of two important domains: the HARE-HTH domain and the Asx homology domain (ASXH). We have then used Circular Dichroism (CD) as a means of investigating the structure of Asxl2. The CD spectra have been measured over a wavelength range from 250 nm to 190 nm at various temperatures, with and without Asxl2’s physiological binding partner, the ubiquity carboxyl-terminal hydrolase BAP1 (Bap1) protein in order to test the hypothesis that Asxl2 is an IDP.
M.S. in Molecular Biochemistry and Biophysics, December 2013
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Title: Plans, specifications and cost of proposed western branch of the southwest land tunnel
Creator: Douthitt, M. J., Pollak, Ernest
Date: 2009, 1908
Publisher: Armour Institute of Technology
Description: http://www.archive.org/details/plansspecificati00dout
Thesis (B.S.)--Armour Institute of Technology

Title: POLITICAL ORIENTATION AS ASSOCIATED WITH STIGMATIZING AND AFFIRMING ATTITUDES TOWARD MENTAL ILLNESS
Creator: Powell, Karina J.
Date: 2013-02-04, 2013-05
Description: Stigma toward people with mental illness can have deleterious effects; employers avoid hiring them, landlords avoid renting to them, and...
Show moreStigma toward people with mental illness can have deleterious effects; employers avoid hiring them, landlords avoid renting to them, and negative attitudes are often a barrier to receiving quality health care. Literature on political ideology indicates that people tend to have fairly stable attitudes over time that are consistent with their political orientation. It is also the case that political ideology is an underlying factor of attitudes toward personal responsibility and hierarchical societal structure. Political ideology, as an underlying framework for attributing controllability through which many individuals view the world, is a demographic variable that needs to be further explored to more completely integrate variances into anti-stigma approaches. Pre-intervention data from a larger study assessing the effects of newspaper articles on attitudes about mental illness were utilized to examine the influence of political orientation on stigmatizing and affirming attitudes toward people with mental illness. Pearson product moment correlations between demographics, political orientation variables, stigmatizing attitudes, and affirming attitudes were conducted. Regression analyses were conducted to determine if political orientation or the interaction between political orientation and demographics are significantly related to stigmatizing attitudes. Results showed that political affiliation emerged as significant associated with pity, danger, blame, and anger when moderated by other demographics suggesting that endorsement of affiliation with a particular political party alone is not sufficient to emerge as related to stigmatizing attitudes, but rather varies at levels of other demographic variables.
M.S. in Psychology, May 2013
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Title: Power consumption of machine tools
Creator: Broyles, J. L., Kupshas, A. C., Rosenberg, L. H.
Date: 2009, 1917
Publisher: Armour Institute of Technology
Description: http://www.archive.org/details/powerconsumption00broy
Thesis (B.S.)--Armour Institute of Technology; Bibliography: leaves 84-85

Title: A proposed design of the steam laboratory for the greater Armour Institute of Technology
Creator: Clouse, J. H, Duennes, F. C
Date: 2009, 1921
Publisher: Armour Institute of Technology
Description: http://www.archive.org/details/proposeddesignof00clou
Thesis (B.S.)--Armour Institute of Technology; Bibliography: leaf 75 B.S. in Mechanical...
Show morehttp://www.archive.org/details/proposeddesignof00clou
Thesis (B.S.)--Armour Institute of Technology; Bibliography: leaf 75 B.S. in Mechanical Engineering, 1921
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Title: A proposed design for the refrigeration laboratory for the greater Armour Institute of Technology
Creator: Hoven, Alfred C, Van Valzah, Robert W.
Date: 2009, 1921
Publisher: Armour Institute of Technology
Description: http://www.archive.org/details/proposeddesignfo00hove
Thesis (B.S.)--Armour Institute of Technology

Title: The production of magnesium by the electrolysis of the fused salts
Creator: Grossman, Alexander N
Date: 2009, 1915
Publisher: Armour Institute of Technology
Description: http://www.archive.org/details/productionofmagn00gros
Thesis (B.S.)--Armour Institute of Technology; Bibliography: leaves 1-21

Title: A proposed design for the hydraulic laboratory for the greater Armour Institute of Technology
Creator: Heitner, William A., Plocar, John J.
Date: 2009, 1921
Publisher: Armour Institute of Technology
Description: http://www.archive.org/details/proposeddesignfo00heit
Thesis (B.S.)--Armour Institute of Technology; Bibliography: leaf 61

Title: The production of potassium chloride and iodine from sea weed
Creator: Green, G. Vernon, Johnson, Harold S
Date: 2009, 1911
Publisher: Armour Institute of Technology
Description: http://www.archive.org/details/productionofpota00gree
Thesis (B.S.)--Armour Institute of Technology

Title: Production of vegetable casein
Creator: Hankan, William M., Friedman, Edwards I.
Date: 2009, 1917
Publisher: Armour Institute of Technology
Description: http://www.archive.org/details/productionofvege00hank
Thesis (B.S.)--Armour Institute of Technology; Bibliography: leaves 47-48

Title: Production of picric acid from monochlorbenzol
Creator: Fitzner, Arthur G, Wollaston, Walter
Date: 2009, 1917
Publisher: Armour Institute of Technology
Description: http://www.archive.org/details/productionofpicr00fitz
Thesis (B.S.)--Armour Institute of Technology; Bibliography: leaf 40

Title: The production of salol from phenol
Creator: Burda, John L., Richards, Harris B.
Date: 2009, 1917
Publisher: Armour Institute of Technology
Description: http://www.archive.org/details/productionofsalo00burd
Thesis (B.S.)--Armour Institute of Technology; Bibliography: leaf 36

Title: The production of metol
Creator: Smith, A. H., Hailey, O. L., Jr.
Date: 2009, 1917
Publisher: Armour Institute of Technology
Description: http://www.archive.org/details/productionofmeto00smit
Thesis (B.S.)--Armour Institute of Technology

Title: Rehabilitation of the municipal electric lighting system at Downers Grove, Ill.
Creator: Drew, H. A., Schuler, C. R.
Date: 2009, 1912
Publisher: Armour Institute of Technology
Description: http://www.archive.org/details/rehabilitationof00drew
Thesis (B.S.)--Armour Institute of Technology

Title: Structural Survey of Select Spectrin Type Repeats within the Rod Region of Dystrophin Using Hydrophobic Moment Analysis and Molecular Dynamics Supplementing Crystallographic Data
Creator: Reinfelds, Pauls Edvins
Date: 2012-07-18, 2012-07
Description: Dystrophin is a rod shaped protein consisting of amino- and carboxy-terminal binding domains linked by a large central rod composed of 24...
Show moreDystrophin is a rod shaped protein consisting of amino- and carboxy-terminal binding domains linked by a large central rod composed of 24 homologous copies of the STR motif and 5 non-homologous regions termed hinges. There are no high-resolution crystallographic structures available for the STR repeats. This work yielded protein crystals and data sets of two STR fragments (d5 & d16-17). Though unable to produce a high resolution structure, this work examines the physical properties of the rod region utilizing hydrophobic moment analysis and molecular dynamics to suggest flexibility among the helices of the STR fragments. Helical shifting appears to possibly occur among the more stable STR tandems to increase the cooperation among certain adjacent fragments. A full understanding of how these properties vary along the length of the rod has implications for the engineering of these rods regions in future dystrophin therapies.
Ph.D. in Biology, July 2012
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Title: Relative hazards of nitrates and chlorates
Creator: Arnold, Charles Harry
Date: 2009, 1913
Publisher: Armour Institute of Technology
Description: http://www.archive.org/details/relativehazardso00arno
Thesis (B.S.)--Armour Institute of Technology

Title: Reduction of ortho-nitro-benzoic acid to anthranilic acid
Creator: Pfafflin, E. W., Winter, E. F.
Date: 2009, 1921
Publisher: Armour Institute of Technology
Description: http://www.archive.org/details/reductionofortho00pfaf
Thesis (B.S.)--Armour Institute of Technology; Bibliography: leaf 36

Title: Relation between rate of combustion and draft pressure for a chain-grain stoker applied to a 350 H.P. Sterling boiler
Creator: Levally, John R., Juttemeyer, Walter L., Wilson, Hubert E
Date: 2009, 1915
Publisher: Armour Institute of Technology
Description: http://www.archive.org/details/relationbetweenr00leva
Thesis (B.S.)--Armour Institute of Technology; Bibliography: leaf 20

Title: The relative cost of operating steam and electric locomotives for switching purposes on the St. Paul railway industry tracks
Creator: Havlick, Spenser N, Sanger, John P, Malwitz, R. C, Albee, T. L
Date: 2009, 1921
Publisher: Armour Institute of Technology
Description: http://www.archive.org/details/relativecostofop00havl
Thesis (B.S.)--Armour Institute of Technology, 1921. B.S. in Mechanical Engineering, 1921

Title: Relation between physical and magnetic hardness of certain carbon steels
Creator: Snowdon, C. R., St. Clair, O. A.
Date: 2009, 1905
Publisher: Armour Institute of Technology
Description: http://www.archive.org/details/relationbetweenp00snow
Thesis (B.S.)--Armour Institute of Technology

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