Elevated glucose and free fatty acids (FFA) are evident in individuals with insulin resistance (IR) and are associated with oxidative stress.... Show moreElevated glucose and free fatty acids (FFA) are evident in individuals with insulin resistance (IR) and are associated with oxidative stress. Anthocyanins are suggested to have favorable effects on human health due to their ability to modulate oxidative stress and cell signaling pathways in peripheral tissues. We hypothesized that strawberry anthocyanins (Str) will improve oxidative stress-mediated impaired insulin signaling in vitro in human skeletal muscle cells (HSMCs). Oxidative stress was generated using physiologically high glucose, FFA or a combination of both for 2–6 h with and without Str (0.1–1.0 mg/mL) in vitro in HSMCs. Cell viability was not affected by any of the above treatments/time. At the end of treatments, cells were treated with insulin (100 nM) for 20 min. Phosphorylation of Akt (p-Akt/Akt) was studied using AlphaScreen® Surefire® assay as a measure of insulin signaling. Our results indicate that FFA (2 mM) or combination of Glucose (15 mM) + FFA (2 mM) significantly decreased p-Akt/Akt compared to control cells treated with phosphate buffered saline (P<0.05). ST extract restored FFA and Glucose + FFA-induced reductions in p-Akt/Akt levels (P<0.05). These in vitro data suggest a role of strawberry in improving insulin sensitivity having potentially important implications for people with IR. M.S. in Biological Sciences, July 2013 Show less
