The flight muscle of the Hawk moth, Manduca sexta, is a synchronous muscle and an emerging model system for structure and function studies of... Show moreThe flight muscle of the Hawk moth, Manduca sexta, is a synchronous muscle and an emerging model system for structure and function studies of muscle. (Tu & Daniel, 2004). There are several interesting properties of Manduca sexta flight muscle. In its physiological characteristics, like mammalian skeletal and cardiac striated muscle, it is a synchronous muscle. However, it is much more similar structurally to the more widely known asynchronous insect flight muscles of Drosophila and Lethocerus. Up to now there has been no good method for storing Manduca sexta flight muscle for a long time. It is often difficult to match the time of obtaining live moths muscle and scheduled X-ray or mechanics experiment. Usually, best results are obtained by using freshly prepared muscle fibers. It would be highly desirable to find storage conditions that can maintain the structural and physiological functions in vitro for days or weeks rather than needing to be prepared fresh from living tissue for every experiment. (Yu-Shu Cheng, MS thesis IIT, 2013). Muscle proteins will degrade over a periods of days even in the presence of protease inhibitors even at low temperature. Here we aimed to find storage conditions which would work best for Manduca sexta flight muscle. In this thesis, trehalose, in combination with glycerol, was evaluated as an addition storage solution as a cryoprotectant to protect the muscle fiber from the low temperature. At very low temperature, organisms became dehydrated. Loss of liquid water from cells can cause irreversible damage, so they will not function even when rehydrated. Here we show that using both glycerol and trehalose as cryoprotectants in storage solution, the muscle fiber maintain high maximum active force for up to a month in storage. M.S. in Biology, December 2015 Show less