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SYNERGISTIC EFFECT OF FATTY ACIDS AND NISIN IN INHIBITING PERSISTER AND BIOFILM OF LISTERIA MONOCYTOGENES
Title
SYNERGISTIC EFFECT OF FATTY ACIDS AND NISIN IN INHIBITING PERSISTER AND BIOFILM OF LISTERIA MONOCYTOGENES
Creator
Zhou, Jiacheng
Date
2019
Description
A foodborne pathogen Listeria monocytogenes causes a life-threatening listeriosis in humans after eating contaminated food. The FDA-approved...
Show moreA foodborne pathogen Listeria monocytogenes causes a life-threatening listeriosis in humans after eating contaminated food. The FDA-approved antimicrobial peptide nisin has been used to prevent contamination of food product from Gram-positive pathogens including L. monocytogenes. However, the formation of biofilms and persisters (i.e., metabolically dormant bacterial population) has resulted in the failure of nisin treatment. Fatty acids, which have been known to exhibit antimicrobial activities, are widely used for therapeutics, food preservation, and agriculture. Previously, we found that two fatty acid compounds lauric acids and N-tridecanoic acids are effective in inhibiting biofilms and persister formation of Gram-negative pathogens. In this study, we investigate whether the fatty acid treatment in combination with nisin promotes inactivation of L. monocytogenes, especially biofilms and persisters. The fatty acid-only treatment reduced the level of biofilms and persisters, while nisin-only treatment resulted in the development of resistant population of L. monocytogenes ATCC19115 strain. However, the co-treatment of the fatty acid and nisin synergistically enhanced the killing of L. monocytogenes by significantly decreasing the number of survived cells and inhibiting biofilms. These results are particularly important in improving food safety in that the food-grade fatty acids can be applied to repress the occurrence of resistant mechanisms of foodborne pathogens by inhibiting biofilm and persister cell formation.
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Establishing Bisphenol A Degradation and Enhancing Microbial Fuel Cell Performance by Biofilm Optimization of Shewanella Oneidensis MR1
Title
Establishing Bisphenol A Degradation and Enhancing Microbial Fuel Cell Performance by Biofilm Optimization of Shewanella Oneidensis MR1
Creator
Zhou, Jiacheng
Date
2023
Description
Bisphenol A (BPA) has been widely used as a plasticizer in the production of synthetic polymers, such as those used in food storage containers...
Show moreBisphenol A (BPA) has been widely used as a plasticizer in the production of synthetic polymers, such as those used in food storage containers and bottles. However, BPA interferes with endocrine systems, causing carcinogenicity, immunotoxicity, and embryotoxicity. Biological water treatment processes scarcely remove BPA, owing to the poor BPA degradability and efficiency of the applied microorganisms. Shewanella oneidensis has been studied and used for the biodegradation process in wastewater treatment because of its excellent extracellular electron transfer properties. In this work, we engineered S. oneidensis MR1 to enable BPA degradation by producing ferredoxin (Fdbisd) and cytochrome P450 (P450bisd) originating from Sphingomonas bisphenolicum AO1. The engineered S. oneidensis exhibited a higher BPA degradation efficiency than that of Escherichia coli producing the same enzymes. The endogenous ferredoxin and ferredoxin reductase of S. oneidensis participated in BPA degradation, and overexpression of mtrC, omcA, and So0521, which encode S. oneidensis cytochromes, decreased BPA. We developed BPA-degrading S. oneidensis biofilms. We measured these optimized BPA-degrading S. oneidensis biofilm in a single chamber microbial fuel cell formed on different carbon electrodes by morphology. Cyclic voltammetry and electrochemical impedance spectroscopy were measured to analyze the biofilm-electrode performance. The biofilm colonization was also measured by confocal laser scanning microscope and scanning electron microscope. And the developed microbial fuel cell was used to degrade BPA and the biofilm developed on different type of carbon anodes was identified. This study provides insights into biocatalyst utilization for the biological degradation of toxic organic compounds.
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