Dr3OR2.1 olfactory receptor is located in the zebrafish chromosome 2. It is initially reported as the only member presented in zebrafish... Show moreDr3OR2.1 olfactory receptor is located in the zebrafish chromosome 2. It is initially reported as the only member presented in zebrafish olfactory receptor genes θ group. The functional study of Dr3OR2.1 is lacking. In order to find out the ligands that might activate Dr3OR2.1, a preliminary chemical screening was conducted in this study. Dr3OR2.1 was cloned from zebrafish cDNA library and inserted into pCI mammalian expression vectors with Rho-tag and without tag. The Dr3OR2.1 was transfected into Hana3A cells and tested for its cell-surface expression by immunocytochemistry. Ten amino acids and four bile acids were tested on Rho tagged and no tag Dr3OR2.1 transfected Hana3A cells. The result indicated that the presence of N terminal Rho-tag may cause the differences between the odorant response profile of these two Dr3OR2.1 constructs. In addition, Dr3OR2.1 is probably an amino acid olfactory receptor rather than a bile acid olfactory receptor. M.S. in Biology, December 2013 Show less
