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Title: CELL-MATRIX INTERACTIONS IN ADIPOSE TISSUE FUNCTION
Creator: Vaicik, Marcella K.
Date: 2015, 2015-07
Description: The overall goal of this work is to apply in vivo and in vitro models to study the basement membrane protein laminin α4 (lama4) in adipocyte...
Show moreThe overall goal of this work is to apply in vivo and in vitro models to study the basement membrane protein laminin α4 (lama4) in adipocyte function. Adipose tissue accumulation, lipogenesis, and structure were examined in mice with a null mutation of the lama4 gene (Lama4−/−) and compared to wild-type animals (Lama4+/+). The Lama4−/−mice phenotype was investigated to evaluate if the differences were due specifically to the adipose tissue function. Physical activity and food intake does not differ between Lama4+/+ and Lama4−/− mice. However, Lama4−/− mice have a significantly increased metabolic rate at 25°C and 16°C compared to Lama4+/+ mice. In contrast, in thermoneutral conditions at 30°C both Lama4+/+ and Lama4−/− mice exhibit equivalent metabolic rates. Interestingly, when room temperature housed mice fat pads were evaluated with immunohistochemistry, Lama4−/− mice exhibit significantly increased UCP-1 expression in subcutaneous adipose. These results suggest that beiging, white to brown adipocytes, in subcutaneous adipose tissue in Lama4−/− mice may lead to decreased adipose tissue accumulation and improved metabolic function. While animal models indicate the absence of lama4 results in more beiging in subcutaneous adipose tissue, an in vitro tissue engineered model was developed to study the adipocyte function in a controlled microenvironment. Primary cell spheroids developed from Lama4+/+ and Lama4−/− were incorporated into synthetic poly(ethylene glycol) (PEG) hydrogels within a range of stiffnesses. When the cells were given the same chemical cues their functions differed depending upon microenvironment stiffness. Beige function in adipocyte cells in 3D can be influenced by matrix stiffness. In conclusion, the laminin alpha 4 basement membrane protein absence in adipose tissue results in adipocyte functional changes in vivo. The Lama4−/− mice have resistance to diet induced weight gain and increased metabolic rate at room temperature and when cold challenged. The Lama4−/− mice had increased beiging in the subcutaneous depot. Additionally, a tissue engineered model was developed to further study cell-ECM interactions first identified in an animal model. These in vivo findings and the engineered model of adipose tissue have great potential for studying obesity and other adipose related diseases. Future work will require continued interdisciplinary collaboration towards the successful identification and screening of novel therapeutics using engineered tissue models.
Ph.D. in Biomedical Engineering, July 2015
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Title: Financial Incubators, India: panel copy
Creator: Shah, Shruti
Date: 2010-05-01, 2010-05

Title: Developing Web Applications for the Northern Illinois Hockey League (sequence unknown), IPRO 308 - Deliverables: IPRO 308 Midterm Presentation F09
Creator: Lucchesi, Kristin, Barksdale, Dustin, Hays, Julian, Bartus, Kryzystof, Perkins, William, Savage, Christopher, Scheer, Colin, Semenov, Vladimir, Vidutis, Mantas
Date: 2009-12
Description: The NIHL is the largest hockey league in Illinois. It is also the largest youth all star hockey league in the US, serving over 300 youth teams...
Show moreThe NIHL is the largest hockey league in Illinois. It is also the largest youth all star hockey league in the US, serving over 300 youth teams and over 4,000 players. The team's purpose is to make the NIHL a web-based scheduling and scoring applications that can support their annual operations.
Sponsorship: Northern Illinois Hockey League(NIHL)
Deliverables for IPRO 308: Developing Web Applications for the Northern Illinois Hockey League for the fall 2009 semester
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Title: A system of multiplex telephony by the use of vacuum tubes
Creator: Arnold, E. H., Throop, A. R.
Date: 2009, 1919
Publisher: Armour Institute of Technology
Description: http://www.archive.org/details/systemofmultiple00arno
Thesis (B.S.)--Armour Institute of Technology; Bibliography: leaves 44-45

Title: FABRICATION, CHARACTERIZATION AND RELEASE STUDIES OF LOW DENSITY POLYETHYLENE/CLAY NANOCOMPOSITES
Creator: Zhao, Ziqian
Date: 2015, 2015-07
Description: Nanocomposites exhibit enhanced physical properties including mechanical strength, thermal stability, and gas barrier properties. The...
Show moreNanocomposites exhibit enhanced physical properties including mechanical strength, thermal stability, and gas barrier properties. The development of nanocomposites has led to applications in food industry, mostly in food packaging. The application of nanocomposites in food packaging may extend the shelf life of a food, thus increasing the market value for manufactures. However, there is limited data and research to assess the risk of exposure of nanomaterials to consumers. This project aims at evaluating the factors that can influence the release of nanomateirals in low density polyethylene/clay nanocomposites films. Nanocomposites based on low density polyethylene (LDPE), containing 1, 3, 5 and 7 wt.% of Cloisite 20A and maleic anhydride-grafted polyethylene (MAPE) as a compatibilizer were prepared by melt compounding and characterized in this thesis project. Cloisite 20A is mostly composed of montmorillonite (MMT), which is commonly used as a nanoscale size filler. LDPE/Clay nanocomposite films containing MMT and three mass equivalents of MAPE were successfully extruded into thin films with good optical clarity. The materials were characterized by using transmission electron microscope (TEM), thermogravimetric analysis (TGA), oxygen permeation analysis and a material testing machine. Important properties such as tensile modulus, oxygen transmission rate, thermal resistance and glass transition temperature were measured. Nanocomposite films were immersed in three kinds of food simulants (absolute ethanol, 3% acetic acid and deionized water). Storage time, storage temperature and percent fill of clay were evaluated as the factors that can influence the release of nanomaterials. The content of released of Al, Mg, and Si was quantified using inductively coupled plasma mass spectrometry (ICP-MS). Mg, Al and Si were continuously released from nanocomposite films in duration of 30 days. Fastest releasing speed was found in 3% aqueous acid at 75 °C, and lower temperature can largely decrease the release of nanoparticles. This project is an initial attempt to assess the risks from the use of nanocomposites as food packaging.
M.S. in Food Safety and Technology, July 2015
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Title: PA 508 Student Computer Usage Survey: PA508 Student Survey 2010 Results
Creator: Ahrens, Aric G.
Date: 2010-01-19, 2010-01-19
Description: Included are a survey, and the results in graphic form. The survey was designed to find out how and where the Chinese contingent of Master of...
Show moreIncluded are a survey, and the results in graphic form. The survey was designed to find out how and where the Chinese contingent of Master of Public Administration students used computers. The focus was to determine whether they preferred to use computers on the Downtown Campus, where classes were taught, or on Main Campus, where most of the students lived.
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Title: Teacher Knowledge Share (Semester Unknown) IPRO 320: Teacher Knowledge Share IPRO 320 MidTerm Presentation F08
Creator: Bern, David, Bochantin, Marike, Hartline, Julian, Mccall, Ian, Mick, Emily, Peake, Andrew, Pierce, Alison, Pindrik, Dmitriy, Quinn, Michael, Stanford, Carly, Tilatti, Michael
Date: 2008, 2008-12
Description: The goal of IPRO 320 is to create an online professional network for school teachers at the kindergarten through twelfth grade level to allow...
Show moreThe goal of IPRO 320 is to create an online professional network for school teachers at the kindergarten through twelfth grade level to allow for the sharing of ideas, lesson plans and other professionally relevant information. This is a continuing project which saw limited success in the previous semester in that a functioning web site was constructed based on surveys of public school teachers but it saw very little use. This semester, the planned approach is to build on the research generated last semester by performing additional market research with teachers. Following that, IPRO 320 will rework much of the previous web site by refining some of the features, adding additional ones as the research indicates and improving the website in general, both functionally and aesthetically.
Deliverables
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Title: SURVIVAL OF SALMONELLA ON CHAMOMILE, PEPPERMINT AND GREEN TEA DURING STORAGE RELATED TO TEMPERATURE AND HUMIDITY
Creator: Chen, Zhengzai
Date: 2013-04-24, 2013-05
Description: Botanical/herbal teas may contain antimicrobial compounds that will affect the survival and persistence of the native as well as contaminant...
Show moreBotanical/herbal teas may contain antimicrobial compounds that will affect the survival and persistence of the native as well as contaminant microflora. Although Salmonella are known for their ability to survive in low water activity environments, their survival on dry botanical/herbal teas has not been examined. The purpose of this work is to determine the survival of Salmonella on three types of teas, chamomile peppermint, and green tea under different storage conditions. In addition, the ability of Salmonella to grow in tea brewed with contaminated product was examined. A cocktail of four Salmonella serovars was inoculated onto 1 g samples of chamomile, peppermint, and green tea to give a starting cell population of approximately 10 log cfu/g. The inoculum was allowed to dry prior to storage at 25 °C and 35 °C and at low (<30% RH) and high (> 90% RH) humidity levels. At different time intervals up to 6 months, triplicate samples at each condition were evaluated for surviving Salmonella populations. In addition, samples were used to produce a low-temperature (sun) brewed tea and inoculated. Growth was assessed after 24 hr. Under the four storage conditions tested, survival followed the order 25°C/low RH > 35°C/low RH > 25°C/high RH > 35°C/high RH. Salmonella losses at 25°C/low RH occurred primarily during drying, after which populations showed little declines over 6 months. In contrast Salmonella decreased below detection after 45 days at 35°C and high RH in all teas tested. All teas tested supported the growth of Salmonella after brewing. Storage conditions significantly impact the survival of Salmonella in dried herbal teas. If Salmonella survives after storage, it may also survive and grow after a home brewing process.
M.S. in Food Safety and Technology, May 2013
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Title: IN VITRO STUDIES OF VIRULENCE SUPPRESSION ON P. AERUGINOSA BY PHOSPHATE / POLYPHOSPHATE-LOADED NANOPARTICLES
Creator: Yin, Yushu
Date: 2015, 2015-07
Description: Critically ill patients harbor multi-drug resistant pathogens that can activate their virulence in the response to low nutrient conditions and...
Show moreCritically ill patients harbor multi-drug resistant pathogens that can activate their virulence in the response to low nutrient conditions and host stress derived factors. It was recently shown that the oversupply of inorganic phosphate to bacterial environment can profoundly suppress the virulence of pathogens. Here we hypothesized that phosphateand/ or polyphosphate-loaded nanoparticles can present a tool to deliver and slowly release phosphate in pathogen-rich niche, thereby suppressing bacterial virulence. In this work, a designed study on effect of different phosphate levels (including the phosphate released from hydrogel nanoparticles) on virulence of P. aeruginosa is addressed. In this work, we developed formulations for preparing hexametaphosphate-loaded nanoparticles on the basis of that for phosphate loaded nanoparticles. We utilized inverse miniemulsion polymerization in the synthesis of these nanoparticles. Polyethylene glycol diacrylate (PEGDA, moleculat weight of 575 Da) and N-vinyl pyrrolidone (molecular weight: 111.14 Da ) were chosen to be the initial monomers because the main crosslinker, polyethylene glycol is a kind of biocompatible material that has been approved by the U.S. Food and Drug Administration (FDA). Several parameter could be adjusted among the experiment. We selected the monomer mole fraction of PEGDA-575 as our parameter. After the synthesis, a nanoparticle size distribution between 110 nm and 150 nm was obtained. And these nanoparticles were proved to be able to release phosphate and hexametaphosphate as drug molecules. Although there were release bursts in the test of release kinetics, the crosslink density could be adjusted in following researches. The second part of this study is to test the virulence suppression effect of the nanoparticles in in vitro experiment on a kind of opportunistic pathogen, P. aeruginosa. This kind of gram-negative bacteria is one of the common intestinal microbial communities. We presented the strategy of suppressing virulence while containing rather than killing the bacteria. As a result, polyphosphate loaded nanoparticles showed to be the most effective one among several experiment groups. This result gave this study a promising future in further research in several aspects, such as in vivo test in biomedical and biomedical engineering.
M.S. in Chemical Engineering, July 2015
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Title: Impacts of Sulfur Capture Technology in Coal Power Plants (sequence unknown), IPRO 302 - Deliverables: IPRO_302_Project_Plan_final
Creator: Contreras, Abraham, Dickman, Justin, Enadeghe, Gregory, Garcia Rodriguez, Hector, Haddad, Michael, Kyle, Ryan, Mongillo, Michael, Murphy, Ryan, Shonubi, Oluwaseun, Swillum, Bryce, Wolber, Brian, Worthon, Terrika
Date: 2009-12
Description: The IPRO team was investigating the net impact of sulfur capture technologies used in current and next generation power plants in the United...
Show moreThe IPRO team was investigating the net impact of sulfur capture technologies used in current and next generation power plants in the United States. It planned to use this knowledge to determine which sulfur capture technologies produce the greatest benefit for industry and society and to find the marketability of the sulfur byproducts created in these power plants.
Sponsorship: Sargent & Lundy, LLC
These are the deliverables for IPRO 302: Impacts of Sulfur Capture Technology in Coal Power Plants from the Fall 2009 semester.
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Title: Transients on transmission lines and modern protective devices
Creator: Muelleman, Joseph P., Smely, James
Date: 2009, 1920
Publisher: Armour Institute of Technology
Description: http://www.archive.org/details/transientsontran00muel
Thesis (B.S.)--Armour Institute of Technology

Title: DEVELOPMENT OF A HIGH ANGULAR RESOLUTION DIFFUSION IMAGING TEMPLATE AND PROBABILISTIC CONNECTIVITY-BASED ATLAS OF THE HUMAN BRAIN
Creator: Varentsova, Anna
Date: 2016, 2016-05
Description: Digital human brain atlases consisting of MRI-based templates and semantic labels delineating different brain regions serve a critical role in...
Show moreDigital human brain atlases consisting of MRI-based templates and semantic labels delineating different brain regions serve a critical role in neuroimaging, mainly facilitating spatial normalization and automated segmentation for the purposes of voxel-wise, region-of-interest, and network analyses. Diffusion MRI templates contain rich information about the microstructure of the brain, however the accuracy of templates constructed based on the diffusion tensor imaging (DTI) model is limited in regions with complex neuronal microarchitecture. High angular resolution diffusion imaging (HARDI) overcomes limitations of the DTI model and is capable of resolving intravoxel heterogeneity. In this work a method to develop artifact-free HARDI template of the human brain from low angular resolution data is presented. Existing white matter (WM) atlases have been generated either based on anatomical landmarks, thus mixing tracts with substantially different roles, or using DTI tractography, which fails in regions with crossing fibers. Connectivity-based atlases developed using HARDI templates and probabilistic tractography have potential to identify functionally distinct subregions of the brain. This work presents connectivity-based atlas of human brain WM created using HARDI template in ICBM-152 space and a set of FreeSurfer grey matter labels.
Ph.D. in Physics, May 2016
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Title: Screech Tones of Supersonic Jets from Bevelled Rectangular Nozzles
Creator: Tam, Ckw, Shen, H., Raman, G.
Date: 1997-07
Publisher: American Inst Aeronaut Astronaut
Description: It is known experimentally that an imperfectly expanded rectangular jet from a thin-lip convergent nozzle emits only a single dominant screech...
Show moreIt is known experimentally that an imperfectly expanded rectangular jet from a thin-lip convergent nozzle emits only a single dominant screech tone. The frequency of the screech tone decreases continuously with an increase in jet Mach number. However, for a supersonic jet issued from a bevelled nozzle or a convergent-divergent nozzle with straight side walls, the shock cell structure and the screech frequency pattern are fairly complicated and have not been predicted before. It is shown that the shock cell structures of these jets can be decomposed into waveguide modes of the jet flow. The screech frequencies are related to the higher-order waveguide modes following the weakest link screech tone theory. The measured screech frequencies are found to compare web with the predicted screech frequency curves.
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Title: Fab Lab: Creating Design-to-Prototype Learning Modules at the Museum of Science and Industry (sequence unknown), IPRO 333 - Deliverables: IPRO 333 Midterm Presentation F09
Creator: Bonesz, Andrew, Clark, Howard, Douglas, Carlie, Gajdorus, Michael, Gottschall, Keenan, Hendricks, Rachel, Kimball, Clayton, Linares, Jared, Marks, Paul, Mellom, James, Oblenida, Cindy, Pop, Sabina, Stelcel, Carl, Young, Jeremy, Zhou, Raymond
Date: 2009-12
Description: The primary objective of IPRO 333 is to partner the knowledge and ideas of young engineers, architects, and scientists at IIT with the...
Show moreThe primary objective of IPRO 333 is to partner the knowledge and ideas of young engineers, architects, and scientists at IIT with the resources of the Museum of Science and Industry (MSI) in Chicago. This partnership endeavors to help the museum's Fabrication Laboratory (Fab Lab) maximize its potential as a resource for museum guests, young and old, as well as for IIT students, staff, and faculty.
Sponsorship: Museum of Science and Industry. Steven Willis- Director, Fabrication Lab
Deliverables for IPRO 333: Fab Lab: Creating Design-to-Prototype Learning Modules at the Museum of Science and Industry for the fall 2009 semester
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Title: Gerry-meandering: An Assessment of the Congressional District Model of Depository Allocation (preprint): AhrensGriffinedrev2
Creator: Ahrens, Aric G., Griffin, Luke A.
Date: 2003-06
Description: The preprint of a published article dealing with the effect of allocating Federal Depositories by Congressional district. Conclusions drawn...
Show moreThe preprint of a published article dealing with the effect of allocating Federal Depositories by Congressional district. Conclusions drawn include the fact that due to redistricting that occurs after each decennial census, over time some districts may have within their boundaries a greater number than the two depositories that Congressional district allocation was intended to produce. Likewise, over time shifting boundaries of districts have created districts that have no depositories whatsoever.
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Title: The yield of acetic acid as influenced by temperature in wood distillation
Creator: Marx, Walter L.
Date: 2009, 1913
Publisher: Armour Institute of Technology
Description: http://www.archive.org/details/yieldofaceticaci00marx
Thesis (B.S.)--Armour Institute of Technology; Bibliography: leaf 22

Title: FIELD AND PHOTO-EMISSION IN A SHORT-PULSE, HIGH-CHARGE CESIUM TELLURIDE RF PHOTOINJECTOR
Creator: Wisniewski, Eric E.
Date: 2014, 2014-05
Description: A new high-charge RF gun is now operating at the Argonne Wakefield Accelerator (AWA) facility at Argonne National Laboratory (ANL). The 1.5...
Show moreA new high-charge RF gun is now operating at the Argonne Wakefield Accelerator (AWA) facility at Argonne National Laboratory (ANL). The 1.5 cell 1.3 GHz gun uses a Cesium telluride photocathode driven with a 248 nm laser to provide short-pulse, high charge electron beams for the new 75 MeV drive beamline. The high-gradient RF gun (peak field on the cathode > 80MV/m) is a key piece of the facility upgrade. The large Cs2Te photocathode (diameter > 30 mm) was fabricated in-house. The photo-injector will be used to generate high-charge, short pulse, single bunches (Q > 100 nC) and bunch-trains (Q>1000 nC) for wakefield experiments, typically involving dielectric-loaded accelerating structures. Details of the photocathode fabrication process and the results of associated diagnostic measurements are presented, including QE measurements and work function measurements performed with a Kelvin probe. Field-emitted dark current from the Cs2Te cathode was measured during RF conditioning and characterized. Fowler-Nordheim plots of the data are presented and compared to similar measurements made using a copper cathode in the initial phase of conditioning. The results for cesium telluride exhibited non-linear regions within the Fowler-Nordheim plots similar to previous experimental results for other p-type semiconductors. Results of quantum efficiency (QE) studies are presented with the cathode operating in both single and bunch-train modes. QE uniformity and lifetime studies are presented. During commissioning, the cesium telluride photocathode produced bunch-charge of 100 nC, breaking the previous record. No evidence of bunch-train position-dependence of QE was found when generating four-bunch trains with total charge up to 200 nC.
PH.D in Physics, May 2014
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Title: SAFETY TRAINING IN CONSTRUCTION
Creator: Demirkesen, Sevilay
Date: 2011-11-29, 2011-12
Description: The construction industry is one of the most hazardous industries. Therefore, the ways to reduce the number of risks have been a concern for...
Show moreThe construction industry is one of the most hazardous industries. Therefore, the ways to reduce the number of risks have been a concern for construction companies. Safety training is considered as one of the most efficient ways of improving safety record. Therefore, this study aimed to describe the best safety training methods and the most efficient organization in safety training. The study also aimed to show how to take best safety measures to protect construction workers. In this thesis study, a questionnaire was conducted in order to investigate the achievement of safety learning, challenges in a safety training program and the methods of improving safety record. Thus, the questionnaire was e-mailed to 400 contractors in US. The data collected also indicated the importance of safety training in companies’ success in the industry. Additionally, this study presents recommendations on how companies could improve their safety record.
M.S. in Civil Engineering, December 2011
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Title: IN VITRO ASSAYS FOR ASSESSING THE IMPACT OF PROCESSING ON THE ALLERGENIC POTENTIAL OF MILK ALLERGENS
Creator: Zhao, Yuhui
Date: 2014, 2014-07
Description: A better understanding of how processing may affect the allergenicity of proteins will allow a more accurate allergen risk assessment and...
Show moreA better understanding of how processing may affect the allergenicity of proteins will allow a more accurate allergen risk assessment and achieve a greater success in food allergy management. To determine whether food processing can affect the allergenic potential of proteins, immunochemical methods are commonly used, however, these methods are not able to determine changes in the proteins’ ability to elicit allergic reactions. Although food challenge studies are the gold standard for determining the allergenicity of proteins, such studies are seldom performed due to ethical reasons and the difficulty in recruiting a sufficient number of study participants. In recent years, a number of mediator release assays have been developed to measure the allergenic potential of food proteins in vitro. How these cell-based assays can be used to assess the impact of thermal or other processing conditions on the allergenic potential of proteins remains to be determined. This study evaluated the use of sensitized rat basophilic leukemia (RBL) cells for assessing the impact of boiling on the allergenic potential of milk allergens by measuring changes in the proteins’ ability to induce cell degranulation and the release of β-hexosaminidase. RBL-2H3 cells sensitized with rat IgEs that were specifically raised against NIST non-fat milk standard reference material, β-lactoglobulin (BLG), α-lactalbumin (ALA) and α-casein (αCAS) were challenged with various concentrations of each allergen in its native and processed (boiled in PBS for 10 min) forms. Cell degranulation caused by the allergen was monitored by measuring the release of β-hexosaminidase. All allergens (NIST milk, BLG, ALA, and αCAS) were able to induce the release of β-hexosaminidase, up to 43.1%. A decrease in % release of β-hexosaminidase was observed when cells were challenged with a decreasing concenration xi of the ALA and milk allergens. Boiling of nonfat milk, ALA, BLG and αCAS caused an increase in the % release of β-hexosaminidase from sensitized cells across antigen concentrations from 10-6 mg/mL to 1 mg/mL. In conclusion, this study demonstrated that the in vitro mediator release assay method may serve as a useful model to evaluate the impact of thermal processing on the allergenic potential of milk allergens.
M.S. in Food Safety and Technology, July 2014
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Title: BIOLOGICAL STRATEGIES FOR ENHANCEMENT OF LIQUID FUELS: SULFUR REMOVAL FROM PETROLEUM AND BIOETHANOL PRODUCTION
Creator: Wang, Jia
Date: 2013, 2013-12
Description: Rhodococcus baikonurensis CW25 was transformed with the Rhodococcus erythropolis strain IGTS8 desulfurization operon (dszABC, which encodes...
Show moreRhodococcus baikonurensis CW25 was transformed with the Rhodococcus erythropolis strain IGTS8 desulfurization operon (dszABC, which encodes the enzymes of the “4S” desulfurization pathway) or this operon modified to contain a synthetic cysteine-methionine rich “sulpeptide” gene (S1) (dszAS1BC). The two CW25 derivatives were subjected to directed evolution to select faster growing cells using the key 4S pathway substrate dibenzothiophene (DBT) as the sole source of sulfur. Data of cell doubling times verified the success of selection of cultures with increasingly rapid growth. The desulfurization activities of resting cells of early passages demonstrated improvements, and the highest activity of the dszAS1BC-bearing CW25 derivative was 115% higher than that of the CW25 derivative without S1. In addition, a trend of initial high activity was followed by a decrease in subsequent passages. Rates of DBT metabolism of growing cells demonstrated a different trend, probably because the activity of growing cells concurrently reflects the activity of DszABC enzymes and the growth rates of the recombinants. Dry cell weights fluctuated during the evolution process, probably because of variations in the efficiency of the conversion of the sulfur in DBT into sulfite, then into sulfate or biomass, or, for the S1-bearing cells, because the secretion of the S1 peptide from cells might have variable efficiency. A mixed culture of two Paenibacillus species (“W” and “Y”) was isolated that can metabolize DBT at temperatures up to 54 ºC. Strain Y is the only one of the two with desulfurization activity, while strain W enhances the desulfurization ability of Y. The W-Y culture may be a useful starting point for selection of desulfurization cultures with even greater thermal stability. xiii Ethanologenic Escherichia coli strain FBR5 was compared with Vitreoscilla hemoglobin (VHb)-expressing FBR5 (TS3) regarding the concentrations of ATP, NAD+, NADH, NAD+/NADH ratio; and growth and ethanol production at various points during growth. The significant finding was that the NAD+/NADH ratio for TS3 was lower in early growth, but higher in later growth compared to that for FBR5. This is probably because more NADH was required by TS3 for its enhanced ethanol production and VHb-related increased respiration under microaeration conditions.
PH.D in Biology, December 2013
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