creator Zhou, Zijin advisor Lee, Alvin Norovirus is one of the leading causes for foodborne illness. Transmission of virus from surface to food has been known to cause a number of outbreaks. Studies of norovirus have been conducted using Murine Norovirus to simulate the behaviors. Pulsed light (PL) is a promising surface decontamination technology, which has the potential to be applied in a food service setting. PL uses intense pulses of short duration and a broad spectrum to accomplish microbial inactivation. This study evaluates the effect of PL on MNV-1, artificially inoculated onto various food contact surfaces including 304 stainless steel, glazed tile, polypropylene, and ultra-high molecular weight (UHMW) polyethylene. The virus was allowed to inoculate on the coupons for 20mins and treated with PL in a Xenon Steripulse XL-3000TM pulsed light treatment system for up to 60 s, at a distance of 8.3 cm 10.8 cm or 13.3cm from the central axis of the lamp. An infrared (IR) camera was used to record surface temperatures, in 1-s increments. After PL treatments, remaining viruses were recovered from surfaces and quantified by plaque assay. At a distance of 10.8cm, MNV-1 was reduced by 2.22-, 2.27- 2.75- and 3.12-log, after 20s treatment on inoculated stainless steel, glazed tile, UHMW polyethylene and polypropylene, respectively. After 50s treatment, MNV-1 was reduced by 4.86- and 5.93- log on glazed tile and stainless steel surface respectively. The surface temperature on tile and stainless steel increased at the rate of 1.08±0.20 and 1.28±0.32°C /s respectively. A relatively short treatment using pulsed light is sufficient to inactivate MNV-1 on the surface of materials commonly used in food preparation. The results suggest that the technology has the potential to reduce surface viral contamination in a food preparation setting. Submitted by Erma Thomas (thomase@iit.edu) on 2016-03-04T17:16:16Z No. of bitstreams: 1 etdadmin_upload_374807.zip: 1311078 bytes, checksum: ac7330286b94f67fb325b2c815e948ee (MD5) Made available in DSpace on 2016-03-04T17:16:16Z (GMT). No. of bitstreams: 1 etdadmin_upload_374807.zip: 1311078 bytes, checksum: ac7330286b94f67fb325b2c815e948ee (MD5) Previous issue date: 2015-07 M.S. in Food Safety and Technology, July 2015 2015 2015-07 http://hdl.handle.net/10560/3744 en Food Contact Surfaces Inactivation Murine Norovirus Norovirus Pulsed light Temperature Thesis born digital application/pdf In Copyright http://rightsstatements.org/page/InC/1.0/ Restricted Access NCFST / National Center for Food Safety and Technology Illinois Institute of Technology Affiliated department