creator Kissinger, Samuel advisor Xiang, Jialing Previous studies have revealed that BAXΔ2, an alternatively spliced form of BAX with a microsatellite mutation (G8 to G7) on BAX exon 3, is a more potent inducer of cell death and a potential means of treatment for cancers with microsatellite instability (MSI). To define the critical splicing points for the splicing of the BAXΔ2 isoform, BAX mini-gene constructs with GFP reporter genes were used for site directed mutagenesis based disruption of predicted critical splicing points. The mutant constructs were transfected into mammalian cells, and expression of BAX-GFP fusion proteins were examined by both fluorescence microscopy and Western blot analysis. Our results indicate that the BAX exon 2 A667T mutation could completely abolish generation of the BAXΔ2 isoform. These results suggest that the acceptor site AG in BAX exon 2 position 667 is critical for alternative splicing to the BAXΔ2 isoform in microsatellite unstable BAX G7 tumor cells. Submitted by Liana Khananashvili (khananashvili@iit.edu) on 2013-10-03T21:16:36Z No. of bitstreams: 2 Samuel T. Kissinger - IIT Masters Thesis 2013.pdf: 1942489 bytes, checksum: 737090c6a3f42b243a97d11da524b3a6 (MD5) Sam Kissinger Signed Title Page.pdf: 197091 bytes, checksum: cf83cf822e7947aac50c9b5c3098ce83 (MD5) Made available in DSpace on 2013-10-03T21:16:36Z (GMT). No. of bitstreams: 2 Samuel T. Kissinger - IIT Masters Thesis 2013.pdf: 1942489 bytes, checksum: 737090c6a3f42b243a97d11da524b3a6 (MD5) Sam Kissinger Signed Title Page.pdf: 197091 bytes, checksum: cf83cf822e7947aac50c9b5c3098ce83 (MD5) Previous issue date: 2013-05 M.S. in Biology, May 2013 2013-04-17 2013-05 http://hdl.handle.net/10560/3069 en Thesis born digital application/pdf In Copyright http://rightsstatements.org/page/InC/1.0/ Restricted Access BIOL / Biology Illinois Institute of Technology Affiliated department