creator Xiong, Rui advisor Dr. Zhang Dr3OR2.1 olfactory receptor is located in the zebrafish chromosome 2. It is initially reported as the only member presented in zebrafish olfactory receptor genes θ group. The functional study of Dr3OR2.1 is lacking. In order to find out the ligands that might activate Dr3OR2.1, a preliminary chemical screening was conducted in this study. Dr3OR2.1 was cloned from zebrafish cDNA library and inserted into pCI mammalian expression vectors with Rho-tag and without tag. The Dr3OR2.1 was transfected into Hana3A cells and tested for its cell-surface expression by immunocytochemistry. Ten amino acids and four bile acids were tested on Rho tagged and no tag Dr3OR2.1 transfected Hana3A cells. The result indicated that the presence of N terminal Rho-tag may cause the differences between the odorant response profile of these two Dr3OR2.1 constructs. In addition, Dr3OR2.1 is probably an amino acid olfactory receptor rather than a bile acid olfactory receptor. Submitted by Liana Khananashvili (khananashvili@iit.edu) on 2014-05-13T15:47:22Z No. of bitstreams: 1 Rui Xiong_A PRELIMINARY STUDY OF THE ODORANTS RECOGNITION BY AN OLFACTORY RECEPTOR DR3OR2.1_Dec, 2013_MS in Biology.pdf: 6529673 bytes, checksum: 88d78041a2fc017dd7da8fd17523da5f (MD5) Made available in DSpace on 2014-05-13T15:47:22Z (GMT). No. of bitstreams: 1 Rui Xiong_A PRELIMINARY STUDY OF THE ODORANTS RECOGNITION BY AN OLFACTORY RECEPTOR DR3OR2.1_Dec, 2013_MS in Biology.pdf: 6529673 bytes, checksum: 88d78041a2fc017dd7da8fd17523da5f (MD5) Previous issue date: 2013-12 M.S. in Biology, December 2013 2013 2013-12 http://hdl.handle.net/10560/3236 en Thesis born digital application/pdf In Copyright http://rightsstatements.org/page/InC/1.0/ Restricted Access BIOL / Biology Illinois Institute of Technology Affiliated department