creator Li, Ying advisor Wang, Rong This thesis demonstrates the formation of micelles from a block copolymer and the application in establishing the micelle based immunosensing system. The utilization of polymeric micelle as probe container and antibody carrier increases the probe to antibody ratio, which is expected to leads to better detection efficiency. The substrate used in the immunosensing is a porous 0.45 μm PVDF membrane which allows the stable immobilization of proteins. Tetraphenylporphyrin, a hydrophobic fluorescent dye, was chosen as the probe encapsulated in micelles. The block copolymer is a self synthesized α-carboxyl-ω-hydroxyl terminated Poly(ethylene oxide)-Polycaprolactone with carboxyl group on the hydrophilic end, permitting the conjugation of antibody to the micelle surface. We choose mouse IgG and its antibody anti-IgG as the pair of protein conjugates to demonstrate the immunoassay. So far, the detection limit of mouse IgG is 1.5 μg/ml (10-8 M) in this immunosensing system, which is expected to improve by modifying the system. Submitted by Liana Khananashvili (khananashvili@iit.edu) on 2013-08-08T20:17:36Z No. of bitstreams: 2 BLOCK COPOLYMER BASED MICELLE FOR EFFICIENT IMMUNOSENSING.pdf: 1833222 bytes, checksum: 6e4b46f764f4ed0c7d43286d829e4eae (MD5) COVER PAGE OF THESIS.PDF: 15090 bytes, checksum: f344c5c3f15a6a91f30224856805f707 (MD5) Made available in DSpace on 2013-08-08T20:17:36Z (GMT). No. of bitstreams: 2 BLOCK COPOLYMER BASED MICELLE FOR EFFICIENT IMMUNOSENSING.pdf: 1833222 bytes, checksum: 6e4b46f764f4ed0c7d43286d829e4eae (MD5) COVER PAGE OF THESIS.PDF: 15090 bytes, checksum: f344c5c3f15a6a91f30224856805f707 (MD5) Previous issue date: 2012-12 M.S. in Chemistry, December 2012 2012-11-26 2012-12 http://hdl.handle.net/10560/3016 en Thesis born digital application/pdf In Copyright http://rightsstatements.org/page/InC/1.0/ Restricted Access CHEM / Chemistry Illinois Institute of Technology Affiliated department