creator Zhou, Jiacheng 2023 Spring 2023 Dissertation Illinois Institute of Technology ChBE / Chemical and Biological Engineering advisor Hong, Seok Hoon advisor Murad, Sohail Chemical engineering Microbiology Biofilm Bisphenol A Carbon electrode Degradation Microbial fuel cell Shewanella oneidensis en Bisphenol A (BPA) has been widely used as a plasticizer in the production of synthetic polymers, such as those used in food storage containers and bottles. However, BPA interferes with endocrine systems, causing carcinogenicity, immunotoxicity, and embryotoxicity. Biological water treatment processes scarcely remove BPA, owing to the poor BPA degradability and efficiency of the applied microorganisms. Shewanella oneidensis has been studied and used for the biodegradation process in wastewater treatment because of its excellent extracellular electron transfer properties. In this work, we engineered S. oneidensis MR1 to enable BPA degradation by producing ferredoxin (Fdbisd) and cytochrome P450 (P450bisd) originating from Sphingomonas bisphenolicum AO1. The engineered S. oneidensis exhibited a higher BPA degradation efficiency than that of Escherichia coli producing the same enzymes. The endogenous ferredoxin and ferredoxin reductase of S. oneidensis participated in BPA degradation, and overexpression of mtrC, omcA, and So0521, which encode S. oneidensis cytochromes, decreased BPA. We developed BPA-degrading S. oneidensis biofilms. We measured these optimized BPA-degrading S. oneidensis biofilm in a single chamber microbial fuel cell formed on different carbon electrodes by morphology. Cyclic voltammetry and electrochemical impedance spectroscopy were measured to analyze the biofilm-electrode performance. The biofilm colonization was also measured by confocal laser scanning microscope and scanning electron microscope. And the developed microbial fuel cell was used to degrade BPA and the biofilm developed on different type of carbon anodes was identified. This study provides insights into biocatalyst utilization for the biological degradation of toxic organic compounds. born digital application/pdf In Copyright http://rightsstatements.org/page/InC/1.0/ Restricted Access http://hdl.handle.net/10560/islandora:1024356