creator HE, QIFAN 2019 Spring 2019 Thesis Illinois Institute of Technology BME / Biomedical Engineering advisor Bhushan, Abhinav Biomedical engineering human hepatocytes Lentivirus PGC1α PPAR-γ rosiglitazone TNFα en In this study, we examined that Proliferator-Activated Receptor γ (PPAR-γ) and Peroxisome Proliferator-Activated Receptor γ Coactivator 1-α (PGC1α) protein expression in hepatocytes have different degrees of expression to Tumor Necrosis Factor α (TNFα) and rosiglitazone. To verify this objective, we employed lentivirus, instead of traditional plasmids, to transfect human hepatocytes (HepG2). Fluorescence-related protein of PPAR-γ and PGC-1α were delivered to hepatocytes, and inflammation was induced by adding TNFα and rosiglitazone to the medium. We successfully designed and created the lentivirus with high delivery efficiency, and determined that the test was true, indicating that PPAR-γ and PGC-1α proteins have different expression to inflammation in human hepatocytes. born digital application/pdf In Copyright http://rightsstatements.org/page/InC/1.0/ Restricted Access http://hdl.handle.net/10560/islandora:1000830